Essentials of Stem Cell Biology. Edition No. 2

Table of Contents

FOREWORD

Why Stem Cell Research?
A New Path ?{ Induced Pluripotent Stem Cells
Embryonic Stem Cells Versus Adult Stem Cells
'Stemness': Definitions, Criteria and Standards

PART ONE
INTRODUCTION TO STEM CELLS
1. Present Perspective and Future Challenges
2. Embryonic Stem Cells in Perspective
3. The Development of Epithelial Stem Cell Concepts
4. The Advent of Direct Reprogramming
5. Clinical Translation of Stem Cells

PART TWO
BASIC BIOLOGY/MECHANISMS
6. Molecular Basis of Pluripotency
7. Stem Cell Niches
8. Mechanisms of Stem Cell Self-Renewal
9. Cell Cycle Regulators in Stem Cells
10. Epigenetic Mechanisms of Cellular Memory
11. Cell Fusion and the Differentiated State
12. How Cells Change Their Phenotype

PART THREE
TISSUE AND ORGAN DEVELOPMENT
13. Differentiation in Early Development
14. Primordial Germ Cells in Mouse and Human
15. Stem Cells in Extraembryonic Lineages
16. Amniotic Fluid Derived Pluripotent Cells
17. Cord Blood Stem Cells
18. Neurogenesis in the Vertebrate Embryo
19. The Nervous System
20. Neuronal Progenitors in the Adult Brain
21. Sensory Epithelium of the Eye and Ear
22. Epithelial Skin Stem Cells
23. The Ontogeny of the Hematopoietic System
24. Hematopoietic Stem Cells
25. Red Blood Cells
26. Cell Differentiation in the Skeleton
27. Vascular Progenitor Cells
28. Multipotent Adult Progenitor Cells
29. Mesenchymal Stem Cells
30. Skeletal Muscle Stem Cells
31. Stem Cells and the Regenerating Heart
32. Potential of ES Cell Differentiation Culture for Vascular Biology
33. Cell Lineages and Stem Cells in the Kidney
34. Adult Liver Stem Cells
35. Pancreatic Stem Cells
36. Stem Cells in the Gastrointestinal Tract
37. Stem Cells in the Lung

PART FOUR
METHODS
38. Generation of Induced Pluripotent Stem Cells
39. Characteristics and Characterization of Pluripotent Stem Cells
40. Isolation and Maintenance of Murine Embryonic Stem Cells
41. Isolation, Characterization, and Maintenance of Primate ES Cells
42. Approaches for Derivation and Maintenance of Human ES cells:
Detailed Procedures and Alternatives
43. Derivation and Differentiation of Human EG Cells
44. Growth Factors & the Serum-Free Culture of Human Pluripotent Stem Cells
45. Feeder-Free Culture
46. Genetic Manipulation of Human Embryonic Stem Cells
47. Homologous Recombination in Human Embryonic Stem Cells
48. Surface Antigen Markers
49. Lineage Marking
50. Genomic Reprogramming
51. Isolation and Characterization of Hematopoietic Stem Cells
52. Microarray Analysis of Stem Cells and Differentiation
53. Zebrafish and Stem Cell Research

PART FIVE
APPLICATIONS
54. Cancer Stem Cells
55. Neural Stem Cells: Therapeutic Applications in Neurodegenerative Diseases
56. Spinal Cord Injury
57. Use of Embryonic Stem Cells to Treat Heart Disease
58. Insulin-Producing Cells Derived from Stem Cells: A Potential Treatment for Diabetes
59. Burns and Skin Ulcers 978-0-12-370465-8
60. Stem Cells and Heart Disease
61. Stem Cells for the Treatment of Muscular Dystropy
62. Skin Regeneration
63. Orthopaedic Applications of Stem Cells
64. Embryonic Stem Cells in Tissue Engineering
65. Adult Stem Cells in Tissue Engineering
66. Stem Cell Gene Therapy

PART SIX
REGULATION AND ETHICS
67. Ethical Considerations
68. Religious Considerations
69. Regulatory Considerations
70. It¡ - s Not About Curiosity, It¡ - s About Cures
Epilogue: Concluding Statement

Authors

Robert Lanza Astellas Institute for Regenerative Medicine, Westborough, MA, USA. Robert Lanza, M.D. is currently Head of Astellas Global Regenerative Medicine, Chief Scientific Officer of AIRM and an adjunct professor at the Wake Forest Institute for Regenerative Medicine. Time magazine recognized him as one of the "100 Most Influential People in the World,” and Prospect magazine named him one of the Top 50 "World Thinkers.” His research focuses on stem cells and their potential to provide therapies for some of the world's most deadly and debilitating conditions. He has hundreds of scientific publications and over 30 books, including definitive references in the fields of tissue engineering and regenerative medicine. He is a former Fulbright Scholar, and studied with polio-pioneer Jonas Salk and Nobel laureates Gerald Edelman and Rodney Porter. He also worked closely (and co-authored a series of papers) with psychologist BF Skinner and heart transplant-pioneer Christiaan Barnard. Dr. Lanza received his undergraduate and medical degrees from the University of Pennsylvania, where he was both a University Scholar and Benjamin Franklin Scholar. Lanza was part of the team that cloned the world's first human embryo, the first endangered species, and published the first-ever reports of pluripotent stem cell use in humans. John Gearhart Johns Hopkins University School of Medicine, Baltimore, MD, USA. Brigid Hogan Duke University Medical Center, Durham, NC, USA. Douglas Melton Howard Hughes Medical Institute, Harvard University, Cambridge, MA, USA. Roger Pedersen Department of Surgery, University of Cambridge, Addenbrooke's Hospital, UK. E. Donnall Thomas Director Emeritus, Fred Hutchinson Cancer Research Center, Seattle, WA, USA. James Thomson Wisconsin Regional Primate Research Center, University of Wisconsin, Madison, USA. Ian Sir Wilmut Director, MRC Centre for Regenerative Medicine, Edinburgh, UK.