Table of Contents
Chapter 1: RNA and the Cellular Biochemistry Revisited
Chapter 2: Transcription and the Organization of Eukaryotic Genes
Chapter 3: Messenger RNA
Chapter 4: Resilient Ribonucleases
Chapter 5: RNA Isolation Strategies
Chapter 6: The Truth About Tissues
Chapter 7: Isolation of Polyadenylated RNA
Chapter 8: Quality Control for RNA Preparations
Chapter 9: Dot Blot Analysis
Chapter 10: Electrophoresis of RNA
Chapter 11: Photodocumentation and Image Analysis
Chapter 12: Northern Analysis
Chapter 13: Nucleic Acid Probe Technology
Chapter 14: Practical Nucleic Acid Hybridization
Chapter 15: Principles of Detection
Chapter 16: Quantification of Specific mRNAs by Nuclease Protection
Chapter 17: Analysis of Nuclear RNA
Chapter 18: cDNA Synthesis
Chapter 19: RT-PCR
Chapter 20: Quantitative PCR Techniques
Chapter 21: Transcript Subtraction Methods
Chapter 22: mRNA Differential Display
Chapter 23: High-Throughput Analysis of Gene Expression
Chapter 24: RNA Interference: Targeted Gene Silencing
Chapter 25: Genomes, Transcriptomes, Proteomes, and Bioinformatics
Chapter 26: An RNA Paradigm
Epilogue: A Few Pearls of Wisdom
Appendix A: Maintaining Complete and Accurate Records
Appendix B: Useful Stock Solutions for the Molecular Biologist
Appendix C: Phenol Preparation
Appendix D: Disposal of Ethidium Bromide and SYBR Green Solutions
Appendix E: DNase I Removal of DNA from an RNA Sample
Appendix F: RNase Incubation to Remove RNA from a DNA Sample
Appendix G: Deionization of Formamide, Formaldehyde, and Glyoxal
Appendix H: Silanizing Centrifuge Tubes and Glassware
Appendix I: Centrifugation as a Mainstream Tool for the Molecular Biologist
Appendix J: Trypsinization Protocol for Anchorage-Dependent Cells
Appendix K: Isolation of High-Molecular-Weight DNA by Salting-Out
Appendix L: RNA Isolation from Plant Tissue
Appendix M: Electrophoresis: Principles, Parameters, and Safety
Appendix N: Polyacrylamide Gel Electrophoresis
Appendix O: Selected Suppliers of Equipment, Reagents, and Services
Appendix P: Useful SI Units
Appendix Q: Common Abbreviations
Appendix R: Trademark Citations
Glossary
Index
