Cytometry is one of the most rapidly growing methodologies available for basic cell and molecular biology, cytogenetics, immunology, oncology, environmental sciences and also various fields of clinical medicine. This new edition, split into 2 Parts, is an almost completely new book, with nearly all of the chapters devoted to new topics. Like the previous volumes on cytometry published as part of the Methods in Cell Biology series, it provides a comprehensive description of particular cytometric methods and reviews their applications. Chapters present the theoretical foundations of the described methods, their applicability in experimental laboratory and clinical settings, and describes common traps and pitfalls such as problems with data interpretation, comparison with alternative assays, and choosing the optimal assay.
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Table of Contents
Section I. New Applications in Cell Biology 1. Recent Advances in Cytometry Applications: Pre-clinical, Clinical, and Cell Biology 2. Detection of Hematopoietic Stem Cells by Flow Cytometry 3. Identification of very small embryonic/epiblast like stem cells (vsels) circulating in peripheral blood during organ/tissue injuries 4. Apoptosis and beyond: cytometry in studies of programmed cell death 5. Assessment of Oxidative Stress-Induced DNA damage by Immunoflourescent Analysis of 8-oxodG 6. Analysis of Individual Molecular Events of DNA Damage Response by Flow and Image Assisted Cytometry 7. Fluorescence-based detection and quantification of features of cellular senescence 8. Measurement of Telomere Length using PNA Probe by Cytometry
Section II. Pre-Clinical and Clinical Applications 9. Cytometry of Intracellular Signaling; From Laboratory Bench to Clinical Application 10. Immunophenotypic pattern of myeloid populations by flow cytometry analysis 11. Flow cytometry-based pharmacodynamic monitoring after organ transplantation 12. Clinical Cytometry and Progress in HLA Antibody Detection 13. Clinical Utility of Flow Cytometry in the Study of Erythropoiesis and Non-Clonal Red Cell Disorders 14. Immunophenotypic characterization of bone marrow MCs in mastocytosis and other mast cell disorders
Author
Darzynkiewicz, Zbigniew.Holden, Elena.
Telford, William.
Wlodkowic, Donald.
