A comprehensive introduction to advanced fluorescence microscopy methods and their applications.
This is the first title on the topic designed specifically to allow students and researchers with little background in physics to understand both microscopy basics and novel light microscopy techniques. The book is written by renowned experts and pioneers in the field with a rather intuitive than formal approach. It always keeps the nonexpert reader in mind, making even unavoidable complex theoretical concepts readily accessible. All commonly used methods are covered.
A companion website with additional references, examples and video material makes this a valuable teaching resource:
Table of Contents
PrefaceIntroduction to Optics and Photophysics (Rainer Heintzmann)
Principles of Light Microscopy (Ulrich Kubitscheck)
Fluorescence Microscopy (Jurek W. Dobrucki)
Fluorescence Labeling (G. Ulrich Nienhaus, Karin Nienhaus)
Confocal Microscopy (Nikolaus Naredi–Rainer, Jens Prescher, Achim Hartschuh, Don C. Lamb)
Fluorescence Photobleaching and Photoactivation Techniques (Reiner Peters)
Förster Resonance Energy Transfer and Fluorescence Lifetime Imaging (Fred S. Wouters)
Single molecule microscopy in the live sciences (Markus Axmann, Josef Madl, Gerhard J. Schütz)
Super–Resolution Microscopy Interference and Pattern Techniques (Gerrit Best, Roman Amberger, Christoph Cremer)
STED Microscopy (Trevis J. Gould, Patrina A. Pellet, Joerg Bewersdorf)
Appendix: Practical guide to optical alignment (Rainer Heintzmann)
