This book is a must-have for anyone interested in obesity or the physiology of white or brown adipose tissues. It contains state-of-the-art methods from researchers that are world leaders in this field. Detailed lab protocols range from methods to visualize adipocytes and adipose tissues in humans and experimental models, to convert stem cells into white and brown adipocytes in vitro, to evaluate aspects of adipocyte metabolism, to inducibly knock out genes in adipose tissues, and to evaluate transcriptional control of adipogenesis on a global scale.
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Table of Contents
1. Preparation and Differentiation of Mesenchymal Stem Cells from Ears of Adult Mice2. 3-D Adipocyte Differentiation and Peri-Adipocyte Collagen Turnover
3. Differentiation of White and Brown Adipocytes from Human Pluripotent Stem Cells
4. Optimal Protocol for the Differentiation and Metabolic Analysis of Human Adipose Stromal Cells
5. Human Adipose Derived Stromal/Stem Cell Isolation, Culture and Osteogenic Differentiation
6. Analysis of Adipose Tissue Lipid Using Mass Spectrometry
7. Measurement of Long-Chain Fatty Acid Uptake into Adipocytes
8. Measurement of the Unfolded Protein Response (UPR) to Investigate its Role in Adipogenesis and Obesity
9. Application of Activity-Based Protein Profiling to Study Enzyme Function in Adipocytes
10. Measurement of Lipolysis
11. Measurement of Lipolysis Products Secreted by 3t3-L1 Adipocytes Using Microfluidics
12. Methods for Performing Lipidomics in White Adipose Tissue
13. Measuring Respiratory Activity of Adipocytes and Adipose Tissues in Real Time
14. Detecting Protein Carbonylation in Adipose Tissue and in Cultured Adipocytes
15. Use of Fluorescence Microscopy to Probe Intracellular Lipolysis
16. Evaluation of Protein Phosphorylation during Adipogenesis
