This new volume of Methods in Cell Biology looks at methods for analyzing correlative light and electron microscopy (CLEM). With CLEM, people try to combine the advantages of both worlds, i.e. the dynamics information obtained by light microscopy and the ultrastructure as provided by electron microscopy. This volume contains the latest techniques on correlative microscopy showing that combining two imaging modalities provides more than each technique alone. Most importantly it includes the essential protocols, including tips, tricks and images for you to repeat these exciting techniques in your own lab. With cutting-edge material, this comprehensive collection is intended to guide researchers for years to come.
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Table of Contents
1. Retracing in Correlative Light Electron Microscopy Where is My Object of Interest? 2. Fluorescing the Electron: Strategies in Correlative Experimental Design 3. Metallothioneins for Correlative Light and Electron Microscopy 4. Correlative Light Microscopy and Electron Tomography to Study Von Willebrand Factor Exocytosis From Vascular Endothelial Cells 5. A Simple Procedure to Analyze Positions of Interest in Infectious Cell Cultures by Correlative Light and Electron Microscopy 6. Correlative Light and Electron Microscopy for a Free-Floating Spindle in Xenopuslaevis Egg Extracts 7. Combining Wide-field Super-resolution Microscopy and Electron Tomography: Rendering Nanoscopic Correlative Arrays on Subcellular Architecture 8. Correlative Cryo-fluorescence and Cryo-soft X-Ray Tomography of Adherent Cells at European Synchrotrons 9. Critical Step-by-step Approaches Toward Correlative Fluorescence/Soft X-Ray Cryo-Microscopy of Adherent Mammalian Cells 10. Correlative Cryo-fluorescence Light Microscopy and Cryo-electron Tomography of Streptomyces 11. Immuno Correlative Light and Electron Microscopy on Tokuyasu Cryosections 12. Step by Step Manipulation of the Cryocapsule with HPM High Pressure Freezers 13. Live-cell CLEM of Sub-cellular Targets: An Optimized Procedure for Polymer Based Imaging Substrates 14. Correlative Fluorescence- and Scanning Transmission Electron Microscopy of Quantum Dot Labeled Proteins on Whole Cells in Liquid 15. The Use of a Laser for Correlating Light and Electron Microscopic Images in Thick Tissue Specimens 16. Correlative In Vivo 2-photon Imaging and Focused Ion Beam Scanning Electron Microscopy: 3D Analysis of Neuronal Ultrastructure 17. Integrated Light and Scanning Electron Microscopy of GFP-Expressing Cells 18. Correlation of the Same Fields Imaged in the TEM, Confocal, LM and MicroCT by Image Registration: From Specimen Preparation to Displaying a Final Composite Image
