The cell interior is another world that we are only beginning to explore. Although there are a number of approaches for examining the inner workings of the cell, the reductionist approach of building up complexity appeals to many with physical science and engineering backgrounds. This volume of Methods in Cell Biology spans a range of spatial scales from single protein molecules to vesicle and cell sized structures capable of complex behaviors. Contributions include; methods for combining different motors and cytoskeletal components in defined ways to produce more complex behaviors; methods to combine cytoskeletal assemblies with fabricated devices such as chambers or pillar arrays; reconstituting membrane fission and fusion; reconstituting important biological processes that normally take place on membrane surfaces; and methods for encapsulating protein machines within vesicles or droplets.
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Table of Contents
1. In Vitro Systems for the Study of Microtubule-Based Cell Polarity in Fission YeastNúria Taberner, Andries Lof, Sophie Roth, Dimitry Lamers, Hans Zeijlemaker and Marileen Dogterom
2. Microtubules, MAPs, and Motor Patterns
Kasimira T. Stanhope and Jennifer L. Ross
3. Self-Organization of Motors and Microtubules in Lipid-Monolayered Droplets
Hella Baumann and Thomas Surrey
4. Reconstitution of Microtubule Based Motility Using Cell Extracts
Swathi Ayloo and Erika L. F. Holzbaur
5. Building Cells for Quantitative, Live-cell Analyses of Collective Motor Protein Functions
Eric A. Kumar, David Tsao, Anand Radhakrishnan and Michael Diehl
6. Reconstituting Cytoskeletal Contraction Events with Biomimetic Actin-Myosin Active gels
José Alvarado and Gijsje H. Koenderink
7. Building an Artificial Actin Cortex on Microscopic Pillar Arrays
R. Ayadi and W. H. Roos
8. Triggering Actin Polymerization in Xenopus Egg Extracts from Phosphoinositide-Containing Lipid Bilayers
Astrid Walrant, Daniel S. Saxton, Guilherme Pereira Correia and Jennifer L. Gallop
9. Reconstituting Geometry Modulated Protein Patterns in Membrane Compartments
Katja Zieske and Petra Schwille
10. Structural and Functional Studies of Membrane Remodeling Machines
Raghav Kalia, Nathaniel Talledge and Adam Frost
11. Building Interconnected Membrane Networks
Matthew A. Holden
12. Using Supported Bilayers to Study the Spatiotemporal Organization of Membrane Bound Proteins
Phuong A. Nguyen, Christine M. Field, Aaron C. Groen, Timothy J. Mitchison and Martin Loose
13. Reconstituting ParA/ParB-Mediated Transport of DNA Cargo
Anthony G. Vecchiarelli, James A. Taylor and Kiyoshi Mizuuchi
14. Cell-Sized Liposomes that Mimic Cell Motility and the Cell Cortex
Joël Lemière, Kevin Carvalho and Cécile Sykes
15. Reconstitution of Cortical Actin Networks Within Water-In-Oil Emulsions
Enas Abu Shah, Maya Malik-Garbi and Kinneret Keren
16. Engineering Artificial Cells by Combining HeLa-Based Cell-Free Expression and Ultra-Thin Double Emulsion Template
Kenneth K.Y. Ho, Victoria L. Murray, Allen P. Liu
17. Reconstitution of Proteins on Electroformed Giant Unilamellar Vesicles
Eva M. Schmid, David L. Richmond, and Daniel A. Fletcher
18. Reconstituting SNARE-Mediated Membrane Fusion at the Single Liposome Level
Volker Kiessling, Binyong Liang and Lukas K. Tamm
