Metabolons and Supramolecular Enzyme Assemblies, Volume 617 in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of topics, including Dynamic plant metabolons, TCA cycle metabolons, the Chemotactic assembly of metabolons, Repurposing peroxisomes for metabolic engineering, Repurposing yeast mitochondria for metabolic engineering, Repurposing plant compartments for metabolic engineering, Protein scaffolds for pathway co-localization on lipid droplets, Engineered enzyme assemblies for metabolic engineering, NRPS assembly lines and P450 interactions, and much more.
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Table of Contents
1. Molecular snapshots of dynamic membrane-bound metabolons Jean-Etienne Bassard and Tomas Laursen 2. Tricarboxylic acid metabolon Fei Wu and Shelley Minteer 3. Metabolon formation by chemotaxis Xi Zhao and Ayusman Sen 4. Cloning, expression, and purification of intact polyketide synthase modules Laurence Maschio, Alice E. Parnell, Nicholas R. Lees, Christine L. Willis, Christiane Schaffitzel, James E.M. Stach and Paul R. Race 5. Repurposing peroxisomes for microbial synthesis for biomolecules Jiaoqi Gao and Yongjin J. Zhou 6. Studying trans-acting enzymes that target carrier protein bound amino acids during nonribosomal peptide synthesis Anja Greule, Louise K. Charkoudian and Max J. Cryle 7. Cargo encapsulation in bacterial microcompartments: Methods and analysis Taylor Nichols, Nolan Kennedy and Danielle Tullman-Ercek 8. Bacterial bioreactors: Outer membrane vesicles for enzyme encapsulation Kendrick B. Turner, Scott N. Dean and Scott A. Walper 9. TXTL-based approach to synthetic cells Jonathan Garamella, David Garenne and Vincent Noireaux 10. Cell-surface display of designer cellulosomes by Lactobacillus plantarum Yonit Ben-David, Sarah Morais, Johanna Stern, Itzhak Mizrahi and Edward A. Bayer 11. Enzyme colocalization in protein-based hydrogels Louis Lancaster, Beyza Bulutoglu, Scott Banta and Ian Wheeldon 12. DNA binding adaptors to assemble proteins of interest on DNA scaffold Eiji Nakata, Huyen Dinh, Thang Minh Nguyen and Takashi Morii 13. Protein-based scaffolds for enzyme immobilization Guoqiang Zhang, Sarah Schmidt-Dannert, Maureen Quin and Claudia Schmidt-Dannert 14. Artificial scaffolds for enhanced biocatalysis Qing Sun, Shen-Long Tsai, and Wilfred Chen 15. On-pot and cell-free biocatalysis using coimmobilized enzymes on advanced materials Fernando López-Gallego 16. Combinatorial biosynthesis of small molecules in plants: Engineering strategies and tools Iryna Gerasymenko, Yuriy Sheludko, Sabine Fräbel, Agata Staniek and Heribert Warzecha 17. Insider information on successful covalent protein coupling with help from SpyBank Anthony H. Keeble and Mark Howarth 18. EMMA assembly explained: A step-by-step guide to assemble synthetic mammalian vectors Sally Jones, Andrea Martella and Yizhi Cai
