Chemical and Synthetic Biology Approaches to Understand Cellular Functions - Part C, Volume 633, the latest release in the Methods in Enzymology series,continues the legacy of this premier serial. This release includes sections on Next generation probes for molecular imaging in cells, Competitive binding assay for biotin and biotin derivatives, based on avidin and biotin-4-fluorescein, Converting avidin to bind ligands other than biotin, especially steroids, Chemoenzymatic Labeling Strategy, Engineered Siderophores, Small molecules to inhibit bacterial population behavior, NMR tube bioreactor, Small molecule controlled RAS activation system, Small molecule regulated Cas9, the Design and application of synthetic receptors, and much more.
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Table of Contents
1. Competitive binding assay for biotin and biotin derivatives, based on avidin and biotin-4-fluorescein Elke Oberbichler, Maria Wiesauer, Eva Schl?gl, Jessica Stangl, Felix Faschinger, G?nther Kn?r, Hermann J. Gruber and Vesa P. Hyt?nen 2. (Strept)avidin as a template for ligands other than biotin: An overview Vesa P. Hyt?nen 3. Engineering siderophores Sina R?tschlin and Thomas B?ttcher 4. Competitive profiling for enzyme inhibitors using chemical probes Michaela Prothiwa and Thomas B?ttcher 5. The NMR tube bioreactor Alexandra V. Chatzikonstantinou, Antonis Tsiailanis, Ioannis P. Gerothanassis, Haralambos Stamatis, Enrico Ravera, Marco Fragai, Claudio Luchinat, Giacomo Parigi and Andreas G. Tzakos 6. A chemically-controlled system for activating RAS GTPases Emily M. Dieter and Dustin J. Maly 7. Temporal and rheostatic control of genome editing with a chemically-inducible Cas9 Cindy T. Wei, Dustin J. Maly and Douglas M. Fowler 8. Synthetic receptors to understand and control cellular functions Hung-Ju Chang and Jerome Bonnet 9. A suite of bioassays to evaluate CREB inhibitors Bingbing X. Li and Xiangshu Xiao 10. Identification of lamins as the molecular targets of LBL1 using a clickable photoaffinity probe Xiangshu Xiao and Bingbing X. Li 11. REX technologies for profiling and decoding the electrophile signaling axes mediated by Rosetta Stone proteins Marcus J. C. Long, Daniel A. Urul and Yimon Aye 12. Building artificial genetic circuits to understand protein function Louis H. Scott, James C. Mathews, Aleksandra Filipovska and Oliver Rackham 13. Methods for studying human sirtuins with activity-based chemical probes Song Zheng, Jessica Wohlfahrt, Ian Cohen and Yana Cen 14. Site-directed labeling of ?-arrestin with monobromobimane for measuring their interaction with G protein-coupled receptors Ashish Srivastava, Mithu Baidya, Hemlata Dwivedi-Agnihotri and Arun K. Shukla 15. Reversible biotinylation of purified proteins for measuring protein-protein interactions Hemlata Dwivedi-Agnihotri, Ashish Srivastava and Arun K. Shukla
