Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Methods for Optical Imaging and Conjugation. Methods in Enzymology Volume 639

Table of Contents

- Fluorogenic detection of protein aggregates in live cells using the AggTag method

Kwan Ho Jung and Xin Zhang

- Synthesis and application of a ratiometric probe for hydrogen peroxide

Ruwen Yin, Yuan Fang, Xinqi Zhou and Cliff I. Stains

- Protein labeling for FRET with methoxycoumarin and acridonylalanine

Chloe M. Jones, Yarra Venkatesh and E. James Petersson

- Ascertaining the activity and inhibition of adenosine deaminase via fluorescence-based assays

Paul T. Ludford III and Yitzhak Tor

- Method to discriminate amyloids using fluorescent probes

Kristine L. Teppang, Rachel S. Ehrlich and Jerry Yang

- Chemical modulation of circadian rhythms and assessment of cellular behavior via indirubin and derivatives

Hui-Hsien Lin, Kelly L. Robertson, Sujeewa S. Lellupitiyage Don, Stephanie R. Taylor and Michelle E. Farkas

- Methods for analysis of near-infrared (NIR) quenched-fluorescent contrast agents in mouse models of cancer

John C. Widen, Martina Tholen, Joshua J. Yim and Matthew Bogyo

- Acid-brightening fluorescent protein (abFP) for imaging acidic vesicles and organelles

Nanxi Wang and Lei Wang

- 4-Cyanoindole-based fluorophores for biological spectroscopy and microscopy

Arusha Acharyya, Ismail A. Ahmed and Feng Gai

- Dissecting contributions of catalytic and reader domains in regulation of histone demethylation

Nektaria Petronikolou, James E. Longbotham and Danica Galonic Fujimori

- Ortho-Phthalaldehyde (OPA)-based chemoselective protein bioconjugation and peptide cyclization

Qing Zhang, Yue Zhang and Xuechen Li

- Chemical synthesis of di-ubiquitin modified histones for further biochemical studies

Jia-Bin Li, Jun Liang and Changlin Tian

- Chemical probes and methods for single-cell detection and quantification of epichaperomes in hematologic malignancies

Swathi Merugu, Sahil Sharma, Justin Kaner, Chander Digwal, Mayumi Sugita, Suhasini Joshi, Tony Taldone, Monica L. Guzman and Gabriela Chiosis

- FlICk (fluorescent isoindole crosslinking) for peptide stapling

Mihajlo Todorovic and David M. Perrin

- Selective protein N-terminal labeling with N-hydroxysuccinimide esters

Hanjie Jiang, Gabriel D. D'Agostino, Philip A. Cole and Daniel R. Dempsey

- Covalent live-cell labeling of proteins using a photoreactive fluorogen

Tewoderos M. Ayele, Steve D. Knutson and Jennifer M. Heemstra

- Photoconvertible diazaxanthilidene dyes for live cell imaging

Rachel M. Lackner, Christopher L. Johnny and David M. Chenoweth

- No-wash live cell imaging with a photoswitchable near-infrared hCRBPII protein-fluorophore tag

Wei Sheng, James H. Geiger and Babak Borhan