Protocols used in Molecular Biology is a compilation of several examples of molecular biology protocols. Each example is presented with a concise introduction, materials, and chemicals required, a step-by-step procedure and troubleshooting tips. Information about the applications of the protocols is also provided. The techniques included in this book are essential to research in the fields of proteomics, genomics, cell culture, epigenetic modification, and structural biology. The protocols can also be used by clinical researchers (neuroscientists and oncologists, for example) for medical applications (diagnostics, therapeutics, and multidisciplinary projects).
Techniques explained in this reference include:
- Nucleic Acid (DNA/RNA) isolation
- Next-generation sequencing through real time PCR
- Western Blotting
- 2-D gel electrophoresis
- Immunohistochemistry
- Chromatin immunoprecipitation (ChIP)
- Live cell-culture techniques
- Golgi Staining
Key Features:
- Recent laboratory protocols
- Diverse examples of molecular biology experiments
- Simple step-by-step presentation of information
- Special focus on scientific and clinical applications
Protocols used in Molecular Biology is essential reading for academicians, molecular biologists, as well as graduate and undergraduate students studying basic and applied research. Pharmacologists and medical researchers can also benefit from the wide array of techniques presented in the book.
Table of Contents
Chapter 1
- Isolation of Genomic DNA from Plant Tissues
Chapter 2
- RNA Isolation Protocol from Cells and Tissues
Chapter 3
- Analyzing Gene Expression Through Real-Time PCR while Neo-Tissue Regeneration Using Developed Tissue Constructs
Chapter 4
- A Modified Western Blot Protocol for Enhanced Sensitivity in the Detection of a Tissue Protein
Chapter 5
- Immunohistochemistry as an Important Technique in Experimental and Clinical Practices
Chapter 6
- Protocols for the Detection and Proteome Analysis of the Yellow Mosaic Virus-Infected Soyabean Leaves
Chapter 7
- 2D-Dige a Powerful Tool for Proteome Analysis
Chapter 8
- Molecular Techniques for Genotyping
Chapter 9
- Sodium Bisulfite Conversion of Human Genome for DNA Methylation Studies
Chapter 10
- Chromatin Immunoprecipitation (Chip)
Chapter 11
- Osteosarcoma Cell Culture and Maintenance to Detect the Apoptotic Effect of Some Promising Compounds by Potent Markers Viz. DNA Fragmentation and Caspase-3 Activation
Chapter 12
- Culture and Maintenance of Human Ovarian Carcinoma Cells for Scrutinizing Anti-Cancerous Activities of Various Compounds Via Some Potent Molecular Markers
Chapter 13
- Dictyostelium Discoideum: Live Cell Imaging in Changing Perspective
Chapter 14
- The Recent Advancement in Rapid Golgi Method and Result Interpretation
Subject Index
Author
- Sandeep Singh
- Dhiraj Kumar
