This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field.
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Table of Contents
- Genome mining and sequence analysis of chemosensory soluble proteins in arthropodsJoel Vizueta, Paula Escuer, Alejandro S?nchez-Gracia and Julio Rozas
- Reconstructing protein coding sequences from ancient DNA
Michael Hofreiter and Stefanie Hartmann
- Bioinformatic, genomic and evolutionary analysis of genes: A case study in Dipteran CSPs
Jean-Fran?ois Picimbon
- Proteomics of arthropod soluble olfactory proteins
Francesca R. Dani and Giuseppe Pieraccini
- Analysis of post-translational modifications in soluble proteins involved in chemical communication from mammals and insects
Andrea Scaloni
- Bacterial expression and purification of vertebrate odorant-binding proteins
Marine Brul?, Margot Glaz, Christine Belloir, Nicolas Poirier, Lucie Moitrier, Fabrice Neiers and Lo?c Briand
- Structure of odorant binding proteins and chemosensory proteins determined by X-ray crystallography
Ana?s Gaubert, B?atrice Amigues, Silvia Spinelli and Christian Cambillau
- Solution structure of insect CSP and OBPs by NMR
Serena Leone, Alessandro Emendato, Roberta Spadaccini and Delia Picone
- Stability of OBPs
Nadja Hellmann
- Ligand-binding assays with OBPs and CSPs
Chiara D'Onofrio, Valeriia Zaremska, Jiao Zhu, Wolfgang Knoll and Paolo Pelosi
- Fine structure immunocytochemistry-An important tool for research on odorant-binding proteins
Rudolf Alexander Steinbrecht
- Effect of OBPs on the response of olfactory receptors
Bing Wang, Song Cao, Wei Liu and Guirong Wang
- Site-directed mutagenesis of odorant-binding proteins
Jiao Zhu, Valeriia Zaremska, Chiara D'Onofrio, Wolfgang Knoll and Paolo Pelosi
- Silencing of OBP genes: Generation of loss-of-function mutants of PBP by genome editing
Yusuke Shiota and Takeshi Sakurai
- Electrochemical biosensing with odorant binding proteins
Sabine Szunerits, Rabah Boukherroub and Alina Vasilescu
- Electronic biosensors based on graphene FETs
Patrik Aspermair, Vladyslav Mishyn, Sabine Szunerits and Wolfgang Knoll
- Electronic biosensors based on EGOFETs
Mohammad Y. Mulla, Luisa Torsi and Kyriaki Manoli
- Gravimetric biosensors
Khasim Cali, Elena Tuccori and Krishna C. Persaud
- Plasmonic biosensors relying on biomolecular conformational changes: Case of odorant binding proteins
Anil Bozdogan, Simone Hageneder and Jakub Dostalek