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Odorant Binding and Chemosensory Proteins. Methods in Enzymology Volume 642

  • Book

  • 514 Pages
  • August 2020
  • Elsevier Science and Technology
  • ID: 5007942

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field.

Please Note: This is an On Demand product, delivery may take up to 11 working days after payment has been received.

Table of Contents

- Genome mining and sequence analysis of chemosensory soluble proteins in arthropods

Joel Vizueta, Paula Escuer, Alejandro S?nchez-Gracia and Julio Rozas

- Reconstructing protein coding sequences from ancient DNA

Michael Hofreiter and Stefanie Hartmann

- Bioinformatic, genomic and evolutionary analysis of genes: A case study in Dipteran CSPs

Jean-Fran?ois Picimbon

- Proteomics of arthropod soluble olfactory proteins

Francesca R. Dani and Giuseppe Pieraccini

- Analysis of post-translational modifications in soluble proteins involved in chemical communication from mammals and insects

Andrea Scaloni

- Bacterial expression and purification of vertebrate odorant-binding proteins

Marine Brul?, Margot Glaz, Christine Belloir, Nicolas Poirier, Lucie Moitrier, Fabrice Neiers and Lo?c Briand

- Structure of odorant binding proteins and chemosensory proteins determined by X-ray crystallography

Ana?s Gaubert, B?atrice Amigues, Silvia Spinelli and Christian Cambillau

- Solution structure of insect CSP and OBPs by NMR

Serena Leone, Alessandro Emendato, Roberta Spadaccini and Delia Picone

- Stability of OBPs

Nadja Hellmann

- Ligand-binding assays with OBPs and CSPs

Chiara D'Onofrio, Valeriia Zaremska, Jiao Zhu, Wolfgang Knoll and Paolo Pelosi

- Fine structure immunocytochemistry-An important tool for research on odorant-binding proteins

Rudolf Alexander Steinbrecht

- Effect of OBPs on the response of olfactory receptors

Bing Wang, Song Cao, Wei Liu and Guirong Wang

- Site-directed mutagenesis of odorant-binding proteins

Jiao Zhu, Valeriia Zaremska, Chiara D'Onofrio, Wolfgang Knoll and Paolo Pelosi

- Silencing of OBP genes: Generation of loss-of-function mutants of PBP by genome editing

Yusuke Shiota and Takeshi Sakurai

- Electrochemical biosensing with odorant binding proteins

Sabine Szunerits, Rabah Boukherroub and Alina Vasilescu

- Electronic biosensors based on graphene FETs

Patrik Aspermair, Vladyslav Mishyn, Sabine Szunerits and Wolfgang Knoll

- Electronic biosensors based on EGOFETs

Mohammad Y. Mulla, Luisa Torsi and Kyriaki Manoli

- Gravimetric biosensors

Khasim Cali, Elena Tuccori and Krishna C. Persaud

- Plasmonic biosensors relying on biomolecular conformational changes: Case of odorant binding proteins

Anil Bozdogan, Simone Hageneder and Jakub Dostalek