Laboratory Practices in Microbiology provides updated insights on methods of isolation and cultivation, morphology of microorganisms, the determination of biochemical activities of microorganisms, and physical and chemical effects on microorganisms. Sections cover methods of preparation of media and their sterilization, microorganisms in environment, aseptic techniques, pure culture techniques, preservation of cultures, morphological characteristics of microorganisms, wet-mount and hanging-drop techniques, different staining techniques, cultural and biochemical characteristics of bacteria, antimicrobial effects of agents on microorganisms, hand scrubbing in the removal of microorganisms, characteristics of fungi, uses of bacteriophages in different applications, and more.
Applications are designed to be common, complete with equipment, minimal expense and quick to the markets. Images are added to applications, helping readers better follow the expressions and make them more understandable. This is an essential book for students and researchers in microbiology, the health sciences, food engineering and technology, and medicine, as well as anyone working in a laboratory setting with microorganisms.
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Table of Contents
About the AuthorPrefaceAcknowledgementsIntroduction to the Student
Section I: Microbiological Media and Cultural Techniques
1. Preparation of Media and Sterilization Techniques2. Microorganisms in Environment3. Aseptic Techniques4. Pure Culture Techniques5. Culture Preservation Techniques
Section II: Techniques on Morphological Examination of Bacteria
6. Microscope for Study of Microorganisms7. Microbial Cell Measurement Technique8. Wet-mount and Hanging-drop Techniques9. Smear Preparation and Simple Staining Techniques10. Gram Staining Technique11. Bacterial Endospore Staining Techniques12. Bacterial Capsule Staining Techniques13. Motility Tests
Section III: Biochemical and Cultural Characteristics of Microorganisms
14. Cultural Characteristics of Bacteria15. Oxidation and Fermentation16. Hydrolytic Reactions
Section IV: Chemical and Physical Effects on Microorganisms
17. Antiseptic and Disinfectant Effects on Microorganisms18. Antibiotic Sensitivity Test Technique19. Effect of Hand Scrubbing on Removal of Microorganisms20. Temperature Effects on Microorganisms21. Osmotic Pressure and pH Effects on Microorganisms22. Ultraviolet Light Effects on Microorganisms23. Oxygen Effects on Microorganisms
Section V: Fungi and Bacteriophages
24. Morphological Characteristics of Yeasts and Molds25. Isolation, Enumeration, and Uses of Bacteriophages
AppendixBibliographyIndex