Analytical Techniques in Biosciences: From Basics to Applications presents comprehensive and up-to-date information on the various analytical techniques obtainable in bioscience research laboratories across the world. This book contains chapters that discuss the basic bioanalytical protocols and sample preparation guidelines. Commonly encountered analytical techniques, their working principles, and applications were presented. Techniques, considered in this book, include centrifugation techniques, electrophoretic techniques, chromatography, titrimetry, spectrometry, and hyphenated techniques. Subsequent chapters emphasize molecular weight determination and electroanalytical techniques, biosensors, and enzyme assay protocols. Other chapters detail microbial techniques, statistical methods, computational modeling, and immunology and immunochemistry.
The book draws from experts from key institutions around the globe, who have simplified the chapters in a way that will be useful to early-stage researchers as well as advanced scientists. It is also carefully structured and integrated sequentially to aid flow, consistency, and continuity. This is a must-have reference for graduate students and researchers in the field of biosciences.
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Table of Contents
1. Bioanalysis: methods, techniques and applications2. Sample preparation techniques in biological and pharmaceutical sciences
3. Centrifugation techniques
4. Electrophoretic techniques
5. Chromatographic techniques: types, principles, and applications
6. Titrimetry
7. Mass Spectrometry
8. Hyphenated techniques
9. Molecular weight determination of nucleic acids and proteins
10. Electroanalytical techniques in biosciences: conductometry, coulometry, voltammetry, and electrochemical sensors
11. Biosensors: components, mechanisms and applications
12. Enzyme assay techniques and protocols
13. Microbial techniques and methods: basic techniques and microscopy
14. Computational modeling and tools in biosciences: bioinformatics approach
15. Statistical methods and tools in biosciences
16. Immunology and immunochemistry
Authors
Chukwuebuka Egbuna Chukwuemeka Odumegwu Ojukwu University, Anambra State, Nigeria.Chukwuebuka Egbuna (PhD) is a chartered chemist and academic researcher. He is a member of the Institute of Chartered Chemists of Nigeria (ICCON), the Nigerian Society of Biochemistry and Molecular Biology (NSBMB), and the Royal Society of Chemistry (RSC) (United Kingdom). Dr. Egbuna is the founder and editor of the Elsevier book series on Drug Discovery Update. The series includes books, monographs, and edited collections from all areas of drug discovery including emerging therapeutic claims for the treatment of diseases. He has published research articles in many international journals of repute and is ranked among the top 500 Nigerian scientists in SciVal/SCOPUS. He has edited more than 25 books with Elsevier, Springer, Wiley, and Taylor & Francis. His most recent book is the three volume Coronavirus Drug Discovery, published by Elsevier. Dr. Egbuna is the founder and the publishing director of IPS Intelligentsia Publishing Services.
Kingsley C. Patrick-Iwuanyanwu Africa Centre of Excellence, Centre for Public Health and Toxicological Research (ACE-PUTOR), University of Port-Harcourt, Rivers State, Nigeria; Department of Biochemistry, University of Port Harcourt, Nigeria. Dr. Kingsley Chukwuemeka Patrick-Iwuanyanwu is a Senior Lecturer in the Department of Biochemistry. Dr. Patrick-Iwuanyanwu is a recipient of The World Academy of Science- International Centre for Chemical and Biological Sciences (TWAS-ICCBS) Postgraduate fellowship award in 2009 to University of Karachi, Pakistan. He was among the 100 young scientists from around the world selected to attend The World Life Sciences Forum by BioVision (Lyon, France); He is a recipient of Society of Toxicology (SOT) /AstraZenecca IUTOX fellowship award in 2009. He served as a pioneer African representative and one of the founding Executive Members of Student Advisory Council (SAC) of Society of Environmental Toxicology and Chemistry (SETAC, EUROPE) from 2009-2011. Dr. Patrick-Iwuanyanwu is a member of several professional bodies including Nigerian Society of Biochemistry and Molecular Biology (NSBMB); Africa Education Initiative (NEF, CT. USA); West African Society of Toxicology (WASOT); Society of Environmental Toxicology and Chemistry (SETAC, Europe); Society of Toxicology (SOT, Reston, USA); Society for Experimental Biology (SEB, UK). Muhammad Ajmal Shah Assistant Professor, Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, Government College University Faisalabad, Pakistan. Dr. Muhammad Ajmal Shah is a registered pharmacist and an academic researcher. He obtained his PharmD and MPhil in Pharmacognosy degrees from Federal Urdu University of Arts, Science & Technology, Pakistan. He obtained his PhD in Pharmaceutical Sciences from Prince of Songkla University Thailand by securing Thailand Education Hub for ASEAN Countries PhD Scholarship Award (TEH-AC 2014-17). He has published over 60 articles in well reputed international journals and has authored over 20 chapters in different books published by Elsevier and Springer. He is a reviewer and editorial board member of various reputed international journals. Dr. Shah is an active member of the American Society of Pharmacognosy, Phytochemical Society of Europe, American Society of Microbiology and GA Society for Medicinal Plant and Natural Product Research Germany. Currently, he is working as an Assistant Professor and Incharge at Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, Government College University Faisalabad, Pakistan. His primary research interests include green extraction and enrichment of bioactive compounds, their isolation and bioevaluation in metabolic and aging associated diseases. Jonathan C. Ifemeje Associate Professor, Department of Biochemistry, Faculty of Natural Sciences, Chukwuemeka Odumegwu Ojukwu University, Nigeria. Dr. Jonathan Chinenye Ifemeje, PhD, is an Associate Professor (Reader) in the Department of Biochemistry, Faculty of Natural Sciences, Chukwuemeka Odumegwu Ojukwu University, Nigeria. He has published over 40 articles with international publishers and has attended series of workshops and conferences. He has also served as an external examiner for various institutions. He is the managing editor of the Tropical Journal of Applied Natural Sciences and a reviewer and an editorial board member for various reputable journals. He has worked extensively in the areas of phytochemistry, environment, and nutritional toxicology. He is a member of various institutes, including the Institute of Chartered Chemists of Nigeria, the Nigerian Society of Biochemistry and Molecular Biology, and Society of Quality Assurance. Dr. Ifemeje is presently the Director of Student Industrial Work Experience Scheme (SIWES), COOU and the Department of Biochemistry Postgraduate Coordinator, COOU. He has also served as the department and faculty staff adviser. He obtained his PhD in applied biochemistry from Nnamdi Azikiwe University, Awka, Nigeria, and his MSc degree in nutrition and toxicology from the University of Port-Harcourt, Nigeria. Azhar Rasul Assistant Professor, Faculty of Life Sciences, Government College University, Faisalabad, Pakistan. Dr. Azhar Rasul is an Assistant Professor at Faculty of Life Sciences, Government College University. He has published over 79 peer-reviewed articles and 4 book chapters. Dr. Rasul has presented several invited talks at National and International level and has attended more than 20 International conferences in Japan, China, South Korea, Dubai, Sri Lanka, Qatar, Turkey, Thailand, Malaysia and France. He has obtained several national and international research grants (HEC-Pak Turk Mobility Grant, ISSESCO RG, HEC-NRPU RG(s), The Nagai Foundation Tokyo RG(s) and COMSTECH-TWAS RG).
