Monitoring Vesicular Trafficking in Cellular Responses to Stress - Part B, Volume 165 in the Methods in Cell Biology series, highlights new advances in the field, with this new release presenting coverage of different topics, including A novel tool for detecting lysosomal membrane permeabilization by high-throughput fluorescence microscopy, Exploring selective autophagy in Drosophila, Assessment of EGFP-Q74 degradation for the measurement of autophagic flux, Multimodal assessment of autophagy in mammalian cells with a novel, LC3-based tandem reporter, Multiplex quantification of autophagic flux by imaging flow cytometry, Monitoring autophagic flux in Caenorhabditis elegans using p62/SQST-1 reporters, High throughput screening for autophagy, and much more.
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Table of Contents
1. A novel tool for detecting lysosomal membrane permeabilization by high-throughput fluorescence microscopy
Karla Alvarez-Valadez, Allan Sauvat, Helene Fohrer-Ting, Christophe Klein, Oliver Kepp, Guido Kroemer, Mojgan Djavaheri-Mergny
2. Exploring selective autophagy in Drosophila
Stavroula Petridi, Anne-Claire Jacomin, Zambarlal Bhujabal, Terje Johansen and Ioannis P. Nezis
3. Assessment of EGFP-Q74 degradation for the measurement of autophagic fluxYan Wang, Jiao Meng, Qi Wu, Guo Chen, Wei Xie, Zhen Zhang, Guido Kroemer and Oliver Kepp
4. Multimodal assessment of autophagy in mammalian cells with a novel, LC3-based tandem reporter
Dan F. Lazar, Amani A. Gillette, Samantha R. Lewis, and Braeden L. Butler
5. Multiplex quantification of autophagic flux by imaging flow cytometry
L�a Montegut, Hui Chen, Gerasimos Anagnostopoulos, Sabrina Spaggiari, Oliver Kepp, Maria Chiara Maiuri, Guido Kroemer, and Isabelle Martins
6. Monitoring autophagic flux in Caenorhabditis elegans using p62/SQST-1 reporters
Christina Ploumi, Aggeliki Sotiriou and Nektarios Tavernarakis
7. High throughput screening for autophagy
Sabrina Forveille, Marion Leduc, Allan Sauvat, Giulia Cerrato, Guido Kroemer and Oliver Kepp
8. Determination of mitophagy by electron microscope
Zhiyu Li, Qi Wu, Le Liu, Si Sun, Shengrong Sun,Zhong Wang, Juanjuan Li
9. Quantification of intracellular ACBP/DBI levels
Valentina Sica, Isabelle Martins, Federico Pietrocola and Jos� Manuel Bravo-San Pedro
10. Immunoblot-based assays for assessing autophagy in the turquoise killifish Nothobranchius furzeri
Gerasimos Anagnostopoulos, Le�a Montegut, Hui Chen, Vincent Carbonnier, Isabelle Martins, Khady Mangane, Abdelkrim Mannioui, Alex Bois, Elisa Elena Baracco, Maria Chiara Maiuri, and Guido Kroemer
11. Monitoring autophagy using super-resolution structured illumination and direct stochastic optical reconstruction microscopy
Dumisile Lumkwana, Lize Engelbrecht and Ben Loos
12. Monitoring selective autophagy of mitochondria using super-resolution microscopy
Ziyue Li and Nicholas T. Ktistakis
13. Monitoring autophagy-dependent ferroptosis
Jingbo Li, Rui Kang, and Daolin Tang
14. Quantitative analysis of autophagy-related protein LC3B by quantum-dot-based molecular imaging
Xin Yu, Juan Wu, Qi Wu, Shengrong Sun
15. Isolation of microRNA from exosomes
Sheila Spada
16. Simultaneous determination of intraluminal lysosomal calcium and pH by dextran-conjugated fluorescence dyes
Philippe Pih�n, Paula Nunes-Hasler, Nicolas Demaurex and Claudio Hetz
