New Methods and Sensors for Membrane and Cell Volume Research, Volume 88 provides an overview of novel experimental approaches to study both the cell membrane and the under-membrane space - the cytosol, which have lately began drawing renewed attention. The book's overall emphasis is on fluorescent and FRET-based sensors, however, other optical (such as variants of transmission microscopy) and non-optical methods (neutron scattering and mass spectrometry) also have dedicated chapters. This volume provides a rare review of experimental approaches to study intracellular phase transitions, as well as anion channels, membrane tension and dynamics, and other topics of intense current interest.
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Table of Contents
On, in, and under membrane
�M.M. Model and I. Levitan
1.�Fluorescence-based sensing of the bioenergetic and physicochemical status of the cell
Luca Mantovanelli, Bauke F. Gaastra and Bert Poolman
2.�Current methods for studying intracellular liquid-liquid phase separation
Amber R Titus and Edgar E Kooijman
3.�Investigating molecular crowding during cell division in budding yeast with FRET
Sarah Lecinski, Jack W. Shepherd, Lewis Frame, Imogen Hayton, Chris MacDonald and Mark C. Leake
4.�The expanding toolbox to study the LRRC8-formed volume-regulated anion channel VRAC
Yulia Kolobkova, Sumaira Pervaiz and Tobias Stauber
5.�Studying cell volume beyond cell volume
Michael A. Model
6.�Membrane tension
Pei-Chuan Chao and Frederick Sachs
7.�Methods for assessment of membrane protrusion dynamics
Jordan Fauser, Martin Brennan, Denis Tsygankov and Andrei V. Karginov
8.�Evaluating membrane structure by Laurdan imaging: Disruption of lipid packing by oxidized lipids
Irena Levitan
9.�Fluorescence sensors for imaging membrane lipid domains and cholesterol
Francisco J. Barrantes
10.�Mass spectrometry-based lipid analysis and imaging
Koralege C. Pathmasiri, Thu T.A. Nguyen, Nigina Khamidova and Stephanie M. Cologna
11.�Deciphering lipid transfer between and within membranes with time-resolved small-angle neutron scattering
Ursula Perez-Salas, Yuri Gerelli, Lionel Porcar and Sumit Garg
