Recombinant Protein Expression, Part B, Volume 660 in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on Multiplexed analysis protein: Protein interactions of polypeptides translated in Leishmania cell-free system, MultiBac system and its applications, performance and recent, Production of antibodies in Shuffle, Designing hybrid-promoter architectures by engineering cis-acting DNA sites to enhance transcription in yeast, Designing hybrid-promoter architectures by engineering cis-acting DNA sites to deregulate transcription in yeast, Antibody or protein-based vaccine production in plants, Cell-free protein synthesis, Plant-based expression of biologic drugs, and much more.
Additional sections cover the Use of native mass spectrometry to guide detergent-based rescue of non-native oligomerization by recombinant proteins, Advancing overexpression and purification of recombinant proteins by pilot optimization through tandem affinity-buffer exchange chromatography online with native mass spectrometry, Method for High-Efficiency Fed-batch cultures of recombinant Escherichia coli, Method to transfer Chinese hamster ovary (CHO) shake flask experiments to the ambr� 250,� and Expression of recombinant antibodies in Leishmania tarentolae.
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Table of Contents
Preface1. Expression of recombinant multi-protein complexes in Saccharomyces cerevisiae
2. Recombinant production of membrane proteins in yeast
3. Expression of proteins in Pichia pastoris
4. Hybrid-architectured promoter design to engineer expression in yeast
5. Hybrid-architectured promoter design to deregulate expression in yeast
Baculovirus-insect cell systems
6. The MultiBac BEVS: Basics, Applications, Performance and Recent Developments
7. Applications of Golden Gate Cloning to protein production using the baculovirus expression vector system
8. A fast-track protocol for protein expression using the BEV system
Plant hosts
9. Transient expression of recombinant proteins in plants
10. Transient expression of recombinant proteins in plants using potato virus X based vectors
11. Design and expression of a bispecific antibody against dengue and chikungunya virus in plants
12. Manufacturing plant-made monoclonal antibodies for research or therapeutic applications
Mammalian cell lines as biomanufacturing platforms
13. Creation of monoclonal antibody expressing CHO cell lines grown with sodium butyrate and characterization of resulting antibody glycosylation
14. Method to transfer Chinese hamster ovary (CHO) batch shake flask experiments to large-scale, computer-controlled fed-batch bioreactors
15. Inducible protein expression in piggyBac transposase mediated stable HEK293 cell pools
16. Exploring the design space of AAV transient-transfection in suspension cell lines
