Fluorophores in Fluorescence Spectroscopy discusses together, for the first time, the basics of fluorescence spectroscopy and the use of fluorophores. Fluorophores are crucial in fluorescence phenomena, especially in the application of fluorescence techniques in various areas such as imaging, sensors and probes. Among the many resources on fluorescence spectroscopy currently available, this is the first to go into detail on fluorophores, serving as a useful tool for the practical application of fluorophores in fluorescence spectroscopy. Written by experts in their various fields of research, the chapters cover everything from fluorophore properties, to synthesis, to applications in biology, physics, and materials science.
Table of Contents
1. Introduction to fluorescence 2. Fluorophores and their design 3. Small fluorophores and their importance 4. Two Photon Absorbing fluorophores 5. Excited state intramolecular proton transfer (ESIPT) 6. BODIPY and azaBODIPY dyes 7. NIR absorbing/Emitting fluorophores 8. Energy transfer (FRET/BRET/CRET) based techniques 9. Fluorescence Lifetime based techniques