Surface enhanced Raman spectroscopy (SERS) is a technique applied in multidisciplinary research. Its use has tremendously grown in the last 40 years owing to improved nanofabrication, biomolecules extraction and sensitive signal acquisition techniques.
This book focuses on the underlying principles of SERS by emphasizing on basic concepts and background information about the subject. Chapters explain the physics of Raman spectroscopy while also indicating its relevance to designing protocols and methodologies for biosensing and imaging. The book gives updated and recent details on colloids and nanostructures, their fabrication, surface engineering and immobilization methods, all in context to SERS based biosensing.
This book serves as a reference book for researchers and academicians and will also provide a reasonable understanding on the topic of SERS to newcomers irrespective of their background in a simple manner. The book is of interest to all readers within the scientific community involved with Raman spectroscopy, including chemists, physicists, biologists, material scientists as well as biomedical engineers.
This book focuses on the underlying principles of SERS by emphasizing on basic concepts and background information about the subject. Chapters explain the physics of Raman spectroscopy while also indicating its relevance to designing protocols and methodologies for biosensing and imaging. The book gives updated and recent details on colloids and nanostructures, their fabrication, surface engineering and immobilization methods, all in context to SERS based biosensing.
Key Features:
- Covers basic knowledge and new research about surface enhanced Raman spectroscopy (SERS)
- Provides a complete framework on SERS based biosensing with concise chapters
- Focuses on different active molecules critical to SERS and associated developed nanoassemblies
- Presents information about ongoing research on SERS imaging applications
- Highlights bottlenecks of SERS technique in biosensing
- Includes references for further reading
This book serves as a reference book for researchers and academicians and will also provide a reasonable understanding on the topic of SERS to newcomers irrespective of their background in a simple manner. The book is of interest to all readers within the scientific community involved with Raman spectroscopy, including chemists, physicists, biologists, material scientists as well as biomedical engineers.
Table of Contents
Chapter 1 State Of The Art: Raman Vibrational Spectroscopy And Surface Enhanced Raman Spectroscopy- Jagjiwan Mittal And Robin Kumar 1.1. Introduction Raman Vibrational Spectroscopy
1.1.2. Basic Theory
1.1.3. Raman Active Vibrations
1.1.4. Rule Of Mutual Exclusion
1.1.5. Raman Spectrometer Instrumentation
1.1.6. Raman Spectrum
1.1.7. Applications Of Raman Spectroscopy
1.1.7.1. Chemical Analysis
1.1.7.2. Solid State Physics
1.1.7.3. Nanotechnology
1.1.7.4. Bio-Pharmaceutical Industry
1.1.7.5. Study Of Historical Painting And Documents
1.1.7.6. Explosives
1.1.7.7. Sensing Based On Raman Spectroscopy
1.2. Surface-Enhanced Raman Scattering (Sers)
1.2.1. Short History
1.2.2. Sers Mechanism
1.2.3. Sers Technique
1.2.4. Sers Substrates
1.2.5. Applications Of Sers
1.2.5.1. Biomarkers
1.2.5.2. Toxic Chemical And Chemical Warfare Agents
1.2.5.3. Narcotic And Doping Drugs
1.2.5.4. Food Monitoring
1.2.5.5. Verification And Authentication Of Luxury Goods And Currency
1.3. Surface Enhanced Resonance Raman Spectroscopy (Serrs)
1.3.1. Advantages Of Serrs Over Sers
- Conclusion
- Consent For Publication
- Conflict Of Interest
- Acknowledgements
- References
Chapter 2 Enhancement Mechanisms And Theory Of Sers
- Pragya Agar Palod And Manika Khanuja 2.1. Introduction
2.2. Enhancement Mechanisms
2.2.1. Electromagnetic Mechanism (Em)
2.2.1.1. Localized Surface Plasmon Resonance (Lspr)
2.2.2. Chemical Mechanism (Chem)
2.2.3. Electronic Mechanism
2.3. Enhancement Factors
2.4. E4 Enhancement
2.5. Factors Affecting Enhancement Factor
2.5.1. Sers Substrate
2.5.2. Wavelength Dependence
2.5.3. Distance Dependence
2.5.4. Dependence On The Size And Shape Of The Nanoparticles
2.6. Two-Photon Excited Sers
2.7. Single Molecule Detection Using Sers
2.8. Hot Spots
2.8.1. Generations Of Hot Spots
- Conclusion
- Consent For Publication
- Conflict Of Interest
- Acknowledgements
- References
Chapter 3 Plasmonic And Non-Plasmonic Resonance Materials In Sers 64 Harsimran Singh Bindra And Ranu Nayak 3.1. Introduction
3.2. Raman Reporter Molecules (Rrm)
3.3. Materials Used In Sers
3.3.1. Plasmonic Sers Nanomaterials
3.3.2. Non-Plasmonic Sers Materials
3.4. Solid Sers Substrates
- Concluding Remarks
- Consent For Publication
- Conflict Of Interest
- Acknowledgements
- References
Chapter 4 Application Of Raman Spectroscopy In Amyloid Research
- Sandip Dolui, Kaushik Bera, Animesh Mondal, Krishnendu Khamaru And Nakul C Maiti 4.1. Introduction
4.1.2. Raman Spectra Of Proteins And Peptides
4.1.3. Raman Techniques In Bio-Analysis
4.2. Amyloid Protein Aggregates And Their Raman Signature
4.2.1. Amyloid Protein Aggregates
4.2.2. Raman Signature Of Amyloid Aggregates Of Human Proteins
4.2.3. Raman Features Of Protein Aggregates Produced By Lysozyme
4.2.4. An Estimation Of Protein Secondary Structure By Amide I Band Analysis
4.2.5. Amyloid Fibril Formation Mechanism By Raman Analysis
- Conclusion And Perspectives
- Consent For Publication
- Conflict Of Interest
- Acknowledgements
- References
Chapter 5 Surface-Enhanced Raman Scattering Based Rapid Pathogen Detection
- Ujjal Kumar Sur And Amar Ghosh 5.1. Introduction
5.3. Sers Based Detection Of Various Biomolecules
5.3.1. Nucleotides And Nucleic Acids
5.3.2. Amino Acids And Peptides/Proteins
5.4. Applications Of Sers In Pathogen Detection
5.5. Raman Spectroscopy Based Detection For Covid-19 Testing
5.6. Limitation Of Sers Technique In Pathogen Detection And Future Trend
- Conclusions
- Consent For Publication
- Conflict Of Nterest
- Acknowledgements
- References
Chapter 6 From Cells To Clinic - Direct Biomolecule Quantification Of Clinically Relevant Biomolecules
- Swati Jain, Harsimran Singh Bindra And Sruti Chattopadhyay 6.1. Introduction
6.3. Neurological Diseases
6.3.1. General Features
6.3.2. Detection Of Neurotransmitters By Sers
6.3.3. New Technique: Surface Enhanced Spatially Offset Raman Spectroscopy (Sesors) 167 6.4. Diabetes-Glucose Monitoring
6.4.1. The Rising Phenomenon Called Diabetes
6.4.2. Continuous Glucose Monitoring
6.4.3. Development Of Sers Sensors For Cgm
6.5. Discreet Cellular Molecules And Cellular Microenvironment 177 6.6. Medical Imaging Through Sers
- Concluding Remarks
- Consent For Publication
- Conflict Of Interest
- Acknowledgements
- References
Chapter 7 Smartphone Based Biosensors On Lateral Flow Assay Coupled To Sers: Point-Of-Care Applications
- Rajasekhar Chokkareddy, Suvardhan Kanchi, Surendra Thakur And Venkatasubba Naidu Nuthalapati 7.1. Introduction
7.1.2. Recent Poc Technologies
7.1.3. Biosensors
7.1.4. Smartphone-Based Biosensors
7.1.5. Challenges Of Accepting Poc In Emergency Department
7.1.6. Influence Of Poc On Clinical Results In The Emergency Department
7.1.7. Essential Features Of Poc Procedures
7.1.8. Surface Enhanced Raman Spectroscopy (Sers) And Its Possible Advantages
7.1.9. Type Of Substrates
7.1.10. What Is It Used For
7.1.11. Usual Sers Investigational System
7.1.12. The Role Of Surface Plasmons And Nanostructures
7.2. Sers Based Dna And Protein Determinations
7.3. Determination Of Other Biologically Related Nanoparticles 205 Conclusions
- Consent For Publication
- Conflict Of Interest
- Acknowledgements
- References
Chapter 8 Surface Enhanced Raman Spectroscopy- Bottlenecks And Improvement Strategies
- Richa Jackeray, Kriti Arya, Zainul Abid Ckv And Harpal Singh 8.1. Introduction
8.1.2. Commercialization
8.2. Improvement Strategies
8.2.1. Tip Enhanced Raman Spectroscopy
8.2.2. Shell Isolated Nanoparticle-Enhanced Raman Spectroscopy
8.2.3. Interference Enhanced Raman Spectroscopy (Iers)
8.2.4. Approach Based On Combinations And Spatio-Temporal Resolution By Nanostructures 199 8.2.5. Improved Nanofabrication
8.2.5.1. Label Method
8.2.5.2. Label-Free Method
8.2.6. Molecular Generality Or Selectivity/Specificity Based Improvement Strategies
8.2.6.1. Reaction-Sers Method
8.2.6.2. Antibody-Sers Method
8.2.6.3. Aptamer-Sers Method
8.2.6.4. Mips-Sers
8.2.6.5. Microfluidics-Sers
8.2.7. Collaborative Experimental And Theoretical Approaches
8.3. Development In Instrumentation
8.4. Handheld Portable Devices
- Conclusion
- Consent For Publication
- Conflict Of Interest
- Acknowledgements
- References
- Subject Index
Author
- Swati Jain
- Sruti Chattopadhyay
