Methods in Cancer Stem Cell Biology: Part A, Volume 170 in the Methods in Cell Biology series highlights advances in the field, with this new volume presenting interesting chapters on timely topics, including Orthotopic brain tumor models derived from glioblastoma stem-like cells, RNA sequencing in hematopoietic stem cells, Generation of inducible pluripotent stem cells from human dermal fibroblasts, In vitro preparation of dental pulp stem cell grafts combined with biocompatible scaffolds for tissue engineering, Gene expression knockdown in chronic myeloid leukemia stem cells, Identification and isolation of slow-cycling GSCs, Assessment of CD133, EpCAM, and much more.
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Table of Contents
1. Orthotopic brain tumor models derived from glioblastoma stem-like cellsKatharina M. Eyme, Alessandro Sammarco, and Christian E. Badr
2. Identification and isolation of slow-cycling glioma stem cells
Liam Furst, Ryan J. Atkins, Marija Dinevska, Stanley S. Stylli, Niall M. Corcoran, Christopher M. Hovens, and Theo Mantamadiotis
3. Immunohistochemical analysis of the developing mouse cortex
Mohamad-Reza Aghanoori, Kaylan M.L. Burns, Maneesha Subha, Laura Williams, Michelle Hua, Farzaneh Nobakht, Taylor Krawec, and Guang Yang
4. Subcellular fractionation of brain tumor stem cells
Ahmad Sharanek, Laura Raco, Vahab D. Soleimani, and Arezu Jahani-Asl
5. microRNAs transcriptional profiling of mammary stem cells isolated by PKH26 staining and FACS sorting
Chiara Tordonato, Matteo Jacopo Marzi, Pier Paolo Di Fiore, and Francesco Nicassio
6 Genome-wide RNA interference screen in cancer stem cells
Guillaume Pinna, Marie Vandamme, Celia Rouault, Emmanuelle Charafe-Jauffret, and Christophe Ginestier
7. Clonal analysis of human clonogenic keratinocytes
Elena Enzo, Camilla Cattaneo, Federica Consiglio, Maria Pia Polito, Sergio Bondanza, and Michele De Luca
8. Immunofluorescence analysis of myogenic differentiation
Atsushi Asakura and Nobuaki Kikyo
9. Generating pancreatic beta-like cells from human pluripotent stem cells
Lillian Yuxian Lim, Carmen Ching, Dewei Kong, Shiao-Yng Chan, and Adrian Kee Keong Teo
10. In vitro preparation of human Dental Pulp Stem Cell grafts with biodegradable polymer scaffolds for nerve tissue engineering
Jose Ramon Pineda, Yurena Polo, Beatriz Pardo-Rodr�guez, Jon Luzuriaga, Ver�nica Uribe-Etxebarria, Patricia Garc�a-Gallastegui, Jos� Ram�n Sarasua, Aitor Larra�aga and Gaskon Ibarretxe
11. Identification of progenitor cells and their progenies in adult Drosophila midgut
Zhen Jin, Minhua Che, and Rongwen Xi
12. Generation and characterization of human mesenchymal stem/stromal cells for cell therapy applications
Luciana M. Dom�nguez, Esteban J. Fiore, and Guillermo D. Mazzolini
13. Generation of sex-reversed female clonal mice via CRISPR/Cas9-mediated Y chromosome deletion in male embryonic stem cells
Yiren Qin, Fuqiang Geng, and Duancheng Wen
