B12 Enzyme, Volume 667 in the Methods in Enzymology serial, highlights new advances in the field with this new volume presenting timely, interesting chapters, including Part I: B12 Biosynthesis and B12 Chaperones, Probing the preference and regioselectivity of lower ligand activation and its role in cobamide diversity, Biosynthesis of corrinoids, The human B12 trafficking chaperones: CblC, CblD and ATR, A method for the efficient adenosylation of corrinoids, Production, purification and liposome reconstitution of cobamide synthase, Extraction and Purification of Cobamides from Bacterial Cultures, Part II: The many faces of B12-dependent enzyme reactions, Methylcobalamin-dependent methionine synthase, and much more.
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Table of Contents
1. B12 Biosynthesis and B12 Chaperones
Martin Warren University of Kent, U.K.
2. Biosynthesis of corrinoids
Amrita Hazra IISER Pune, India
3. Guardian of cobamide diversity: probing the role of CobT in lower ligand activation in the biosynthesis of vitamin B12 and other cobamide cofactors
Michiko Taga University of California, Berkeley
4. Purification and Detection of Vitamin B12 Analogs
Jorge Escalante-Semerena University of Georgia
5. A method for the efficient adenosylation of corrinoids
Jorge Escalante-Semerena University of Georgia
6. A method for the production, purification and liposome reconstitution of cobamide synthase
Jorge Escalante-Semerena University of Georgia
7. A method for the Isolation of� -ribazole from Vitamin B12, and its Enzymatic Conversion to -ribazole 5'-phosphate
Ruma Banerjee University of Michigan
8. The human B12 trafficking chaperones: CblC, CblD and ATR
Bernhard Kr�utler University of Innsbruck, Austria
9. Synthesis and applications of "antivitamin" B12 derivatives B12-dependent enzyme reactions
Tetsuo Toraya Okayama University, Japan
10. Coenzyme B12-dependent eliminases: Diol and glycerol dehydratases and ethanolamine ammonia-lyase
Tetsuo Toraya Okayama University, Japan
11. Reactivating chaperones for coenzyme B12-dependent eliminases: Diol and glycerol dehydratases and ethanolamine ammonia-lyase
Wolfgang Buckel Marburg University
12. Glutamate mutase and 2-methyleneglutarate mutase
Ruma Banerjee University of Michigan
13. Human B12 dependent enzymes: Methionine synthase and methylmalonyl-CoA mutase
David Leys Manchester University
14. Heterologous production and biophysical characterisation of catabolic Nitratireductor pacificus pht-3B reductive dehalogenase
Nigel Scrutton Manchester University
15. An unusual light-sensing function for coenzyme B12 in bacterial transcription regulator CarH
