Carotenoids: Carotenoid and Apocarotenoid Biosynthesis, Metabolic Engineering and Synthetic Biology, Volume 671, the latest release in the Methods of Enzymology series highlights new advances in the field with chapters on Metabolomics-based analysis of carotenoids and related metabolites in various species via quantitative trait loci and genome wide association mapping approaches, Using bacteria for functional analysis of genes encoding carotenoid biosynthetic enzymes, Rice Callus as a High Throughput Platform for Synthetic Biology and Metabolic Engineering of Carotenoids, Transient expression in Nicotiana benthamiana: A simple platform to investigate genes encoding carotenoid biosynthesis enzymes from diverse algal lineages, and much more.
Additional chapters in this new release cover Protein-protein interaction techniques to investigate post-translational regulation of carotenogenesis, The isolation of sub-chromoplast structures from tomato and capsicum fruit, Carrot protoplasts as a suitable method for protein subcellular localization, High throughput production and characterization of carotenoid enzymes for structural and functional studies, Production and structural characterization of the cytochrome P450 enzymes in carotene ring hydroxylation, and more.
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Table of Contents
1. Metabolomics-based analysis of carotenoids and related metabolites in various species via quantitative trait loci and genome wide association mapping approaches2. Enzymatic isomerization of ?-carotene mediated by the heme-containing isomerase Z-ISO
3. Using bacteria for functional analysis of genes encoding carotenoid biosynthetic enzymes
4. Rice Callus as a High Throughput Platform for Synthetic Biology and Metabolic Engineering of Carotenoids
5. Transient expression in Nicotiana benthamiana: A simple platform to investigate genes encoding carotenoid biosynthesis enzymes from diverse algal lineages
6. Protein-protein interaction techniques to investigate post-translational regulation of carotenogenesis
7. The isolation of sub-chromoplast structures from tomato and capsicum fruit
8. Carrot protoplasts as a suitable method for protein subcellular localization
9. High throughput production and characterization of carotenoid enzymes for structural and functional studies
10. Production and structural characterization of the cytochrome P450 enzymes in carotene ring hydroxylation
11. Characterizing CYTOCHROME P450 Enzymes Involved in Plant Apocarotenoid Metabolism by Using Engineered Yeast System
12. Preparation of carotenoid cleavage dioxygenases for crystallography
13. Heme-dependent enzymes involved in carotenoids biosynthesis in plants
14. Tracking sub-plastidic localization of carotenoid metabolic enzymes with proteomics
15. Golden Rice and lessons learned for inspiring future metabolic engineering strategies and synthetic biology solutions
16. Genetic transformation of Cassava to improve carotenoids: a protocol revisited
17. Elevating fruit carotenoid content in apple (Malus x domestica Borkh)
18. Engineering microbial synthesis of carotenoids
19. The breeder's tool-box for enhancing the content of esterified carotenoids in wheat: from extraction and profiling of carotenoids to marker-assisted selection of candidate genes
20. Drawing on enzyme promiscuity to create novel carotenoids via non-phytoene precursors
21. Plant abscisic acid receptor agonists and antagonists
22. A synthetic biology approach to study carotenoid production in Corynebacterium glutamicum: read-out by a genetically encoded biosensor combined with perturbing native gene expression by CRISPRi
23. Designing biosensors from a plant apocarotenoid receptor
24. Imaging Retinaldehyde-Protein Binding in Plants using a Merocyanine Reporter 25. Use of directed enzyme evolution to create novel biosynthetic pathways for production of non-natural and rare carotenoids
