Small Angle Scattering, Part A: Methods for Structural Investigation, Volume 675 in the Methods in Enzymology series, highlights new advances in the field, with new chapters in this updated release including SAXS foundations and metrics, Contrast variation sample preparation protocols, experimental procedures, and rudimentary analysis, Molecular deuteration for neutron scattering, Planning, Executing and Assessing the Feasibility of SANS Contrast Variation Experiments, Technical considerations for small-angle neutron scattering from biological macromolecules, and Advanced sample environments and capabilities at our synchrotron X-ray beamline with example applications.
Additional sections in the book cover SEC-SAXS-MALS data acquisition and processing pipeline at SIBYLS, SEC-SAXS: pros and cons, experimental set-up, examples and software developments, Radiation damage and sample economy for stopped-flow methods in the time regime of millisecond and above, Stopped-flow-time-resolved SAXS, Insights on Temp-jump, time-resolved SAXS, and much more.
Please Note: This is an On Demand product, delivery may take up to 11 working days after payment has been received.
Table of Contents
1. SAXS foundations and metricsRobert Rambo
2. Contrast variation sample preparation protocols, experimental procedures, and rudimentary analysis
Lois Pollack
3. Molecular deuteration for neutron scattering
Anthony Patrick Duff
4. Planning, Executing and Assessing the Feasibility of SANS Contrast Variation Experiments
Susan Krueger
5. Technical considerations for small-angle neutron scattering from biological macromolecules
Cy Jeffries and Andrew Whitten
6. Advanced sample environments and capabilities at our synchrotron X-ray beamline with example applications
Melissa Graewert and Dmitri Svergun
7. SEC-SAXS-MALS data acquisition and processing pipeline at SIBYLS
Daniel Rosenberg and Michal Hammel
8. SEC-SAXS: pros and cons, experimental set-up, examples and software developments
Patrice Vachette, Javier Perez and Aurelien Thureau
9. Radiation damage and sample economy for stopped-flow methods in the time regime of millisecond and above
Michael Weiss
10. Stopped-flow-time-resolved SAXS
Henning Tidow and Inokentijs Josts
11. Insights on Temp-jump, time-resolved SAXS
Philip Anfinrud
12. Time-resolved Small-Angle Neutron Scattering (TR-SANS) for structural biology of dynamic systems: history, recent developments, and practical guidelines
Frank Gabel
13. SAXS data collection for Protein Fibrillation
Bente Vestergaard and Annette Langkilde
14. Actin assembly
Kristina Djinovic Carugo
15. SAS induced disulfide bond damage
Edward Snell
16. High pressure SAXS
Richard Gillilan
17. Challenges and opportunities in soft X-ray scattering of biological molecules
Esther W. Gomez
18. Collection of scattering data from lipid membranes and nanodiscs
Lin Yang
19. SANS stealth methods for membrane proteins
Henning Tidow and Inokentijs Josts
20. Predicting solution scattering patterns with explicit-solvent molecular simulations
Jochen S. Hub
21. From dilute to concentrated solutions of intrinsically disordered proteins: Sample preparation and data collection
Marie Skepo
22. Extracting physically-relevant ensemble properties of disordered proteins and other polymers from SAXS data
Joshua A. Riback
23. Determining topological structures of RNA using SAXS
Yun Xing Wang
24. SAS data analyses of polydisperse systems with special emphasis on transient complexes
Pau Bernado and Aamin Sagar
25. FEL samples and data collection
Peter Zwart
