Radiation Oncology and Radiotherapy, Part A, Volume 172 in the Methods in Cell Biology series, highlights advances in the field, with this new volume presenting interesting chapters on timely topics, including DNA damage quantification by the COMET assay, Immunofluorescence microscopy-assisted quantification of ATM and ATR activation in irradiated cells, Immunoblotting-based characterization of the DNA damage response, Assessment of lipid peroxidation in irradiated cells, A simple method to assess clonogenic survival of irradiated cancer cells, Quantification of beta-galactosidase activity as a marker of radiation-driven cellular senescence, Cytofluorometric assessment of cell cycle progression in irradiated cells, and more.
Other sections cover Assessment of transcription inhibition as a characteristic of immunogenic cell death, Assessment of eIF2? phosphorylation during immunogenic cell death, Quantification of cytosolic DNA species by immunofluorescence and automated image analysis, Flow cytometry-assisted quantification of CALR exposure during immunogenic cell death, Interference of immunogenic anticancer therapy by artificially controlled calreticulin secretion from tumor cells, along with many additional topics of interest.
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Table of Contents
1. DNA damage quantification by the COMET assay
Mathias Weyland
2. Immunofluorescence microscopy-assisted quantification of ATM and ATR activation in irradiated cells
Ilio Vitale
3. Immunoblotting-based characterization of the DNA damage response
Ilio Vitale
4. Assessment of lipid peroxidation in irradiated cells
Boyi Gan, Chao Mao, Amber Horbath and Guang Lei
5. A simple method to assess clonogenic survival of irradiated cancer cells
Maria Esperanza Rodriguez Ruiz
6. Quantification of beta-galactosidase activity as a marker of radiation-driven cellular senescence
Giulia Petroni
7. Cytofluorometric assessment of cell cycle progression in irradiated cells
Giulia Petroni, Ai Sato, Aitziber Buqu� Mart�nez and Lorenzo Galluzzi
8. Assessment of transcription inhibition as a characteristic of immunogenic cell death
Guido Kroemer, Oliver Kepp, Giulia Cerrato and Allan Sauvat
9. Assessment of eIF2? phosphorylation during immunogenic cell death
Guido Kroemer, Oliver Kepp and Lucillia Bezu
10. Quantification of cytosolic DNA species by immunofluorescence and automated image analysis
Lorenzo Galluzzi
11. Flow cytometry-assisted quantification of CALR exposure during immunogenic cell death
Jitka Fucikova
12. Interference of immunogenic anticancer therapy by artificially controlled calreticulin secretion from tumor cells
Guido Kroemer, Oliver Kepp, Peng LIU and Liwei Zhao
13. Assessment of type I interferon responses� as a feature of immunogenic cell death.
Guido Kroemer, Oliver Kepp, Sabrina Forveille, Allan Sauvat and Liwei Zhao
14. Assessment of immunological memory formation in vivo
Guido Kroemer, Oliver Kepp, Liwei Zhao and Peng LIU
15. Characterization of the SASP of cancer cells experiencing DNA damage
Francis Rodier
16. ELISA-based quantification of type I IFN secretion by irradiated cancer cells
Claire I. Vanpouille-Box
17. RT-PCR-assisted quantification of type I IFN responses by irradiated cancer cells
Lorenzo Galluzzi
18. Methods to characterize the exosomal output of irradiated cancer cells
Sheila Spada
19. Cytofluorometric assessment of acute cell death responses driven by radiation therapy
Lorenzo Galluzzi
