Carotenoids: Biological Functions of Carotenoids and Apocarotenoids in Natural and Artificial Systems, Volume 674 in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on topics such as Ultrafast laser spectroscopic studies on carotenoids in solution and on those bound to photosynthetic pigment-protein complexes, Assessing photoprotective functions of carotenoids in photosynthetic systems of plants and green algae, Fluorescence of carotenoids: probing binding site interactions and conformational motion in carotenoproteins, Resonance Raman: A powerful tool to interrogate carotenoids in biological matrices, and much more.
Other chapters in the book cover Engineering the carotenoid biosynthetic pathway to study the function of carotenoids in light-harvesting complexes, Carotenoids as proxies for variations in photosynthesis and phenology in response to environmental and climatic change, Apocarotenoid pigment biosynthesis in non-model plants, Apocarotenoid transport in plants, Screening for apocarotenoid plant growth regulators in Arabidopsis, Effects of herbivory on carotenoid biosynthesis and breakdown, Biosynthesis and action of apocarotenoid plant hormones, and much more.
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Table of Contents
1. Ultrafast laser spectroscopic studies on carotenoids in solution and on those bound to photosynthetic pigment-protein complexes
Hideki Hashimoto and Richard Cogdell
2. Assessing photoprotective functions of carotenoids in photosynthetic systems of plants and green algae
Roberto Bassi
3. Fluorescence of carotenoids: probing binding site interactions and conformational motion in carotenoproteins
Warren Beck and Justin Rose
4. Resonance Raman: A powerful tool to interrogate carotenoids in biological matrices
Bruno Robert, Bruno Robert, Manuel Llansola Portoles and Andrew A. Pascal
5. Engineering the carotenoid biosynthetic pathway to study the function of carotenoids in light-harvesting complexes
Andrew Hitchcock, Neil Hunter, David John Kenneth Swainsbury, Pu Qian and George Sutherland
6. Carotenoids as proxies for variations in photosynthesis and phenology in response to environmental and climatic change
Ingo Ensminger
7. Apocarotenoid pigment biosynthesis in non-model plants
Giovanni Giuliano
8. Apocarotenoid transport in plants
Giovanni Giuliano
9. Screening for apocarotenoid plant growth regulators in Arabidopsis
Salim Al Babili, Kunpeng Jia, Alexandra Jazz Dickinson, Yagiz Alagoz and Jianing Mi
10. Effects of herbivory on carotenoid biosynthesis and breakdown
Jonathan Gershenzon and Sirsha Mitra
11. Biosynthesis and action of apocarotenoid plant hormones
12. Strigolactone Signaling Complex Formation in Yeast: A Paradigm for Studying Hormone-induced Receptor Interaction with Multiple Downstream Proteins
Ruifeng Yao, Li Chen, Meng Zhang, Haiyang Yu, Liu Yang, Hongfan Long and Xin Su
13. Assessment of dietary carotenoid intake and metabolism in human clinical studies
Steven Clinton and Elizabeth Grainger
14. Microsomal Triglyceride Transfer Protein-mediated transfer of beta-carotene from Donor to Acceptor vesicles in vitro
Loredana Quadro, Jahangir Iqbal, Mahmood Hussain and Youn-Kyung Kim
15. Development and validation of a method to deliver vitamin A into macrophages
Jaume Amengual, Pooja Acharya, Molly Black and Glenn Bressner
16. Methods for assessing the interaction of apocarotenoids with vertebrate nuclear receptors
Earl Howard Harrison
17. A guide for the evaluation of in vitro bioaccessibility of carotenoids
Adriana Z. Mercadante and Ana Augusta O. Xavier
18. A fast and simplified method to estimate bioaccessibility of carotenoids from plant tissues
Manuel Rodriguez-Concepcion and Luca Morelli
19. From organic synthesis of novel carotenoids and apo-carotenoids via metabolism to bio-active carotenoid metabolites and their activity relevance in mice and humans
Ralph R�hl, Angel R. de Lera, Torsten Bohn, Jean-Francois Landrier, Harald Carlsen, Daniel Merk, Jenny Renaut and Tilman Todt
20. Extraction, detection, and imaging of the macular carotenoids
Paul S. Bernstein, Binxing Li, Aruna Gorususpudi and Ranganathan Arunkumar
21. Analysis of macular carotenoids in the developing Macaque retina: The timeline of macular pigment development
John Landrum, Vanes Mendez, Yisi Cao, Ramon Gomez and Nartha Neuringer
22. Carotenoid Modifying Enzymes in Metazoans
Johannes F. von Lintig, Alexander R. Moise and Sepalika Bandara
23. Expression and Biochemical Analyses of Proteins Involved in the Transport of Carotenoids and Retinoids
Alexander R. Moise and Johannes F. von Lintig
