Integrated Methods in Protein Biochemistry: Part A, Volume 677, the latest release in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on topics such as DNA and protein engineering to create protein bioswitches with new functions, Interaction and cross-talk of prelamin A with integral membrane zinc metalloproteases, An experimental protocol to study lipid transfer proteins, Synthesis of small heat shock proteins, Druggable p-p interacting sites for Co-chaperone DNAJA1 and its partner proteins, An experimental protocol for glycoconjugate analysis, Methods for proximity-based biotinylation combined with Mass Spectrometry, and more.
Additional chapters cover Synthetic antibody fragments as conformational sensors of protein activation and trafficking, Expression, purification, functional analysis and crystallization of Rag GTPase, Purification of bacterial transcription elongation complexes by photoreversible immobilization, Inhibition of c-Myc-MAX heterodimerization, Fluorogenic RNA aptamers to probe transcription by multi-subunit RNA polymerases, and much more.
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Table of Contents
1. DNA and protein engineering to create protein bioswitches with new functionsStewart Loh
2. Interaction and cross-talk of prelamin A with integral membrane zinc metalloproteases
Susan Michaelis
3. An experimental protocol to study lipid transfer proteins
Haijia Yu
4. Synthesis of small heat shock proteins
Matthew Pratt
5. Druggable p-p interacting sites for Co-chaperone DNAJA1 and its partner proteins
Guang-Yu Yang
6. An experimental protocol for glycoconjugate analysis
Yifa Zhou
7. Methods for proximity-based biotinylation combined with Mass Spectrometry
Ralph Kehlenbach
8. Synthetic antibody fragments as conformational sensors of protein activation and trafficking
Arun K. Shukla
9. Expression, purification, functional analysis and crystallization of Rag GTPase
Kuang Shen
10. Purification of bacterial transcription elongation complexes by photoreversible immobilization
Eric Strobel
11. Inhibition of c-Myc-MAX heterodimerization
Neha Garg
12. Fluorogenic RNA aptamers to probe transcription by multi-subunit RNA polymerases
Markus C. Wahl
13. Phosphorylation and subcellular localization of human phospholipase A1
Atsushi Yamashita
14. Integrated methods in studying light-sensitive proteins
Lars-Oliver Essen
15. Structure and biochemistry of thermophilic protein
Ankan Banerjee
16. Using hydrogen/deuterium exchange mass spectrometry to probe protein structure
Gert Bange
17. Nanopore force spectroscopy to probe structural dynamics of proteins
Sonja Schmid
18. Structural analysis of knotted proteins
Shang-Te "Danny" Hsu
19. Mutation-induced structural changes in the SARC-CoV2 spike protein
Shang-Te "Danny" Hsu
20. Preparing metabolite capped RNA substrates using RNA polymerases
Jeremy G. Bird
