The Immunological Synapse, Part A, Volume 173 in the Methods in Cell Biology series provides state-of-the-art methods for the study of the immunological synapse. Sections cover Imaging polarized granule release at the cytotoxic T cell immunological synapse using TIRF microscopy: control by polarity regulators, Analysis of centrosomal area actin reorganization and centrosome polarization upon lymphocyte activation at the immunological synapse, P815-based redirected degranulation assay to study human NK cell effector functions, Cytotoxic and Chemotactic Dynamics of Natural Killer Cells Quantified by Live-cell Imaging, Quantification of interaction frequency between antigen-presenting cells and T cells by conjugation assay, and more.
Other chapters focus on the Study of the Effects of NK-Tumor Cell Interaction by Proteomic Analysis and Imaging, Quantification of lymphocytic choriomeningitis virus specific T cells and LCMV viral titers, Quantification of lymphocytic choriomeningitis virus specific T cells and LCMV viral titers, An in vitro model to monitor natural killer cell effector functions against primary breast cancer, and Standardized Protocol for the Evaluation of Chimeric Antigen Receptor (CAR)-modified Cell Immunological Synapse Quality using the Glass-supported Planar L.
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Table of Contents
- Imaging polarized granule release at the cytotoxic T cell immunological synapse using TIRF microscopy: Control by polarity regulators
Marie Juzans, Celine Cuche, Vincenzo Di Bartolo, and Andres Alcover - Analysis of centrosomal area actin reorganization and centrosome polarization upon lymphocyte activation at the immunological synapse
Sara Fernandez-Hermira, Irene Sanz-Fernandez, Marta Botas, Victor Calvo, and Manuel Izquierdo - P815-based redirected degranulation assay to study human NK cell effector functions
I�igo Terren, Gabirel Astarloa-Pando, Ainhoa Amarilla-Irusta, and Francisco Borrego - Cytotoxic and chemotactic dynamics of NK cells quantified by live-cell imaging
Yanting Zhu and Jue Shi - Quantification of interaction frequency between antigen-presenting cells and T cells by conjugation assay
Ondrej Cerny - Assessment of membrane lipid state at the natural killer cell immunological synapse
Yu Li and Jordan S. Orange - Study of the effects of NK-tumor cell interaction by proteomic analysis and imaging
Chiara Lavarello, Paola Orecchia, Andrea Petretto, Massimo Vitale, Claudia Cantoni, and Monica Parodi - Protocol for the murine antibody-dependent cellular phagocytosis assay
Eliana Stanganello, Magdalena Brkic, Steven Zenner, Ines Beulshausen, Ute Schmitt, and Fulvia Vascotto - Quantification of lymphocytic choriomeningitis virus specific T cells and LCMV viral titers
Melanie Grusdat, Catherine Dostert, and Dirk Brenner - An in vitro model to monitor natural killer cell effector functions against breast cancer cells derived from human tumor tissue
Nicky A. Beelen, Femke A.I. Ehlers, Loes F.S. Kooreman, Gerard M.J. Bos, and Lotte Wieten - Standardized protocol for the evaluation of chimeric antigen receptor (CAR)-modified cell immunological synapse quality using the glass-supported planar lipid bilayer
Jong Hyun Cho, Wei-chung Tsao, Alireza Naghizadeh, and Dongfang Liu - Potency monitoring of CAR T cells
Dongrui Wang, Xin Yang, Agata Xella, Lawrence A. Stern, and Christine E. Brown
