Radiation Oncology and Radiotherapy, Part B, Volume 174 in the Methods in Cell Biology series, highlights advances in the field, with this new volume presenting interesting chapters on timely topics including CT-assisted focal irradiation of tumors in mice, Methods to preserve correct dosimetry in small animal irradiators, Monitoring TGFbeta signaling in irradiated tumors, Cytofluorometric characterization of the lymphoid compartment of irradiated tumors, Cytofluorometric characterization of the myeloid compartment of irradiated tumors, Mass cytometry to characterize the immune infiltrate of irradiated tumors, Characterization of the immune infiltrate in irradiated mouse tumor by multiplex immunofluorescence, and much more.
Other chapters cover Methods to study FOXP3+CD8+ cells in irradiated bone metastases, Luminex-based characterization of cytokine signaling by irradiated tumors, Explant-based assessment of anticancer immunity in irradiated tumors, Pipeline to characterize the TCR repertoire of irradiated tumors, Pipeline to identify tumor neoantigens exposed by radiation, and more.
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Table of Contents
- Flow cytometry-assisted quantification of immune cells infiltrating irradiated tumors in mice
Sara Labiano, Irantzu Serrano-Mendioroz, and Mar�a Esperanza Rodriguez-Ruiz - Cytofluorometric characterization of the myeloid compartment of irradiated mouse tumors
Marine Gerb� De Thor�, Lydia Meziani, Eric Deutsch, and Michele Mondini - Mass cytometry to characterize the immune lung cancer microenvironment
Ti Badri, I�aki Eguren-Santamaria, Eva Fernandez-Pierola, and Miguel F. Sanmamed - Characterization of the immune infiltrate in mouse tissue by multiplex immunofluorescence
Teresa Marafioti, Maria D. Lozano, and Carlos E. de Andrea - Ex vivo analysis of radiation effects on tumor infiltrating immune cells using tumor explants
Aanchal Preet Kaur, Gwen Kramer, Marka R. Crittenden, and Michael J. Gough - Assessment of immunological memory formation in vivo
Shuai Zhang, Liwei Zhao, Hui Chen, Guido Kroemer, Peng Liu, and Oliver Kepp - MorphoMacro for in vivo and ex vivo quantitative morphometric analysis of microglia
Pedro Melo, Mariana S. Silveira, In�s Mendes-Pinto, and Jo�o B. Relvas - Quantitative assessment of mitophagy in irradiated cancer cells
Emma Guilbaud, Sheila Spada, Norma Bloy, Claudia Galassi, Ai Sato, Carlos Jim�nez-Cortegana, Artur Aretz, Aitziber Buqu�, Takahiro Yamazaki, Sandra Demaria, and Lorenzo Galluzzi - Quantification of beta-galactosidase activity as a marker of radiation-driven cellular senescence
Vanessa Klapp, Norma Bloy, Giulia Petroni, and Mara De Martino - A simple method to assess clonogenic survival of irradiated cancer cells
Irantzu Serrano-Mendioroz, Eneko Garate-Soraluze, and Mar�a Esperanza Rodriguez-Ruiz - Clonogenic assay to measure bystander cytotoxicity of targeted alpha-particle therapy
Julie Constanzo, Clara Diaz Garcia-Prada, and Jean-Pierre Pouget
