Methods in Enzymology serial highlights new advances in the field with this new volume presenting interesting chapters. Each chapter is written by an international board of authors.
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Table of Contents
1. Fluorescence loading assay for hexameric helicasesJames Berger
2. Use of substrates with multiple fluorophores to simultaneously monitor unwinding of multiple duplexes
Alicia Byrd
3. In silico reconstitution of MCM helicase loading and activation using time resolved cryo-EM
Alessandro Costa
4. Helicase uncoupling to study nacent strand decay
James Dewar
5. Investigating structure and dynamics of Twinkle helicase with Cryo-EM and high-speed AFM
Yang Gao
6. Measuring helicase contributions to BIR
Greg Ira
7. Crosslinking to define helicase/DNA interactions
James L. Keck?
8. Use of Reverse Polarity DNA to investigate Helicase Mechanisms
Tim Lohman
9. Substrate production to study helicases and translocases using molecular and optical tweezers
Fernando Moreno Herrero
10. Methods of loading CMG onto DNA
Michael O'Donnell
11. Methods to study Helicase-Polymerase coupling during DNA replication reactions
Smita Patel
12. Biochemical analyses of ATP-dependent chromatin remodeling
Craig Peterson
13. Alignment of helicases on single-stranded increases activity
Kevin D. Raney
14. CMG helicase activity on G4-containing templates
Dirk Remus
15. Accessory helicase unwinding of genomic complexities
Grant Schauer
16. Monitoring helicase decoupling in bacteria by TUNEL
Michael Trakselis
17. the preparation of DNA constructs for the use in single-molecule replisome studies
Antoine Van Oijen
18. Interaction of topoisomerases with DNA measured by RADAR
Alessandro Vindigni