Polysaccharide-Degrading Biocatalysts provides a thorough grounding in these biocatalytic processes and their growing role in the depolymerization of polysaccharides, empowering researchers to discover and develop new enzyme-based approaches across pharmaceuticals, fuels, and food engineering. Here, over a dozen leading experts offer a close examination of structural polysaccharides, genetic modification of polysaccharides, polysaccharide degradation routes, pretreatments for enzymatic hydrolysis, hemicellulose-degrading enzymes, biomass valorization processes, oligosaccharide production, and enzyme immobilization for the hydrolysis of polysaccharides, among other topics and related research protocols. A final chapter considers perspectives and challenges in an evolving, carbohydrate-based economy.
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Table of Contents
1. Plant cell wall polysaccharides: Methodologies for compositional, structural, and physicochemical characterization 2. Genetic modification of plants to increase the saccharification of lignocellulose 3. The diversity of plant carbohydrate hydrolysis in nature and technology 4. State-of-the-art experimental and computational approaches to investigate structure, substrate recognition, and catalytic mechanism of enzymes 5. Pretreatments as a key for enzymatic hydrolysis of lignocellulosic biomass 6. Importance of accessory enzymes in hemicellulose degradation 7. How ligninolytic enzymes can help in the degradation of biomass polysaccharides, cleavage, and catalytic mechanisms? 8. Biochemical and biotechnological aspects of microbial amylases 9. Hydrolysis of complex pectin structures: Biocatalysis and bioproducts 10. Macroalgal polysaccharides: Biocatalysts in biofuel/bioenergy production 11. Mathematical modeling of the enzymatic hydrolysis of polysaccharides: A primer 12. Polysaccharide deconstruction products: Production of bio-based building blocks 13. Polysaccharide degradation for oligosaccharide production with nutraceutical potential for the food industry 14. Carbohydrate-active enzymes in the production of lactose-derived tagatose 15. Immobilized biocatalysts for hydrolysis of polysaccharides 16. Carbohydrate-based economy: Perspectives and challenges
