Diagnostic Molecular Biology, Second Edition provides an up-to-date comprehensive overview of the latest advances in molecular diagnostics and the fundamental molecular aspects in this area. The book delves into the essential principles behind the transmission and expression of genetic information, including nucleic acids, proteins, and chromosomes, specifically their genome structure, life cycle, and evolution. The latest methods and instrumentation in the field are examined with detailed procedures and explanations provided for each technique. A range of techniques are also investigated, such as those used in sequencing analysis, genome and transcriptome analysis, and nucleic acid amplitification techniques. This book covers the collection, isolation, and purification of DNA, RNA, and protein from different sources. Additionally, discussion on the applications of next-generation sequencing (NGS) and the role of NGS in the future of precision medicine is included. The book also examines the use of molecular diagnostics for chromosomal disorders, inherited diseases, and infectious diseases. Moreover, this new edition features recent advances in the detection of respiratory virus infections in humans, including that of SARS-CoV2. Diagnostic Molecular Biology, Second Edition, is an ideal reference for researchers, lab technicians, and students in medical laboratory science, molecular biology, microbiology, virology, and related fields.
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Table of Contents
1. Nucleic Acids: DNA and RNA 2. Nucleic Acid-Based Cellular Activities DNA Replication, Damage, and Repair 3. Gene Expression: Transcription of the Genetic Code 4. Gene Expression: Translation of the Genetic Code 5. The Genome 6. Extraction and Purification of Nucleic Acids 7. Quantification and Analysis of Nucleic Acids 8. Extraction and Purification of Proteins 9. Quantification and Analysis of Proteins 10. Amplification of Nucleic Acids 11. Characterization of Nucleic Acids and Proteins 12. Techniques in Sequencing 13. Genome and Transriptome Analysis 14. Molecular Diagnosis of Chromosomal Disorders 15. Molecular Diagnosis of Gene Mutation and Inherited Diseases 16. Molecular Diagnosis of Infectious Diseases 17. The Biology of SARS-CoV-2 and the Molecular Diagnosis of COVID-19 18. Guidance for Molecular Diagnostic Laboratory
Authors
Chang-Hui Shen Professor of Biology and Chair of the Biology Department at the College of Staten Island, City University of New York, New York, USA. Chang-Hui Shen, PhD, is a Professor of Biology and Biochemistry at the College of Staten Island and the Graduate Center, City University of New York. He earned his B.S. and M.S. at the National Chung-Hsing University, Taiwan, and his Ph.D. at the University of Edinburgh, UK. He was a visiting fellow of the National Institutes of Health before joining the City University of New York.Dr. Shen's research focuses on the mechanism of gene expression, with an emphasis on epigenetic regulation in gene activation. His lab utilizes the breadth of current and advanced techniques in molecular biology and biochemistry. He has published dozens of articles throughout his career, and his research has been supported by several agencies, including NATO, NIH, and NSF.
