Cilia: From Mechanisms to Disease, Part A, Volume 175 in the Methods in Cell Biology series, offers a range of techniques and protocols that can be used to study aspects of this interesting cellular organelle both in vitro and in vivo. Sections in this new release include Protocols to induce and study ciliogenesis, Flow Cytometry-Based Approach for the study of primary Cilia, Microscopic observation of human airway ciliary movement using wheat germ agglutinin, Time-lapse imaging of primary cilia behavior with physiological expression of fluorescent ciliary proteins, Evaluation of ciliary-GPCR dynamics using a validated organotypic brain slice culture method, and much more.
Other sections cover Studying the morphology, composition and function of the photoreceptor primary cilium in zebrafish, Visualizing Multiciliated Cells in the Zebrafish, Isolation of Ciliary Ectosomes and Analysis of Peptide-mediated Chemotaxis in Chlamydomonas, Using Paramecium as a Model for Ciliopathies, Using organoids to study cilia, Using in vivo cerebellar electroporation to study neuronal cell proliferation and differentiation in a Joubert syndrome mouse model, and more.
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Table of Contents
1. Protocols to induce and study ciliogenesisJose Manuel M. Bravo San Pedro
2. A Flow Cytometry-Based Approach for the study of primary Cilia
Francesca Ciccolini
3. Microscopic observation of human airway ciliary movement using wheat germ agglutinin
Yo Kishimoto
4. Time-lapse imaging of primary cilia behavior with physiological expression of fluorescent ciliary proteins
Koji Ikegami
5. Evaluation of ciliary-GPCR dynamics using a validated organotypic brain slice culture method
Yumiko Saito and Yuki Kobayashi
6. High-efficient CRISPR/Cas9-mediated gene targeting to establish cell models of ciliopathies
Tatsuo Miyamoto, Kosuke Hosoba, Tomoka Morita, Ying Zhang, Hiroko Kishi and Takashi Yamamoto
7. Studying the morphology, composition and function of the photoreceptor primary cilium in zebrafish
Ruxandra Bachman-Gagescu
8. Visualizing Multiciliated Cells in the Zebrafish
Rebecca Ann Wingert
9. Isolation of Ciliary Ectosomes and Analysis of Peptide-mediated Chemotaxis in Chlamydomonas
Stephen M. King
10. Using Paramecium as a Model for Ciliopathies
Judith Van Houten
11. Using organoids to study cilia
Vincent GUEN
12. Using in vivo cerebellar electroporation to study neuronal cell proliferation and differentiation in a Joubert syndrome mouse model
Tang K. Tang, Ting-Yu Chen and Chia-Hsiang Chang
