Volume Electron Microscopy (vEM), Volume 177 is a collective term for a set of three-dimensional high-resolution ultrastructural imaging techniques that have delivered new insights into biological systems in recent years, garnering substantial interest in the life and clinical sciences. In this book, users will find a variety of vEM workflows and technologies, highlighting application areas with biologically relevant examples. Topics covered include Automated large volume sample preparation for vEM, Resin comparison for Serial Block Face Scanning Volume Electron Microscopy, Immunolabelling for SBF-SEM, Electron Microscopy in Plants, Serial section electron tomography, Automated Tape Collecting Ultramicrotomy (ATUM) for targeting neuropathology, Array tomography, and much more.
Other sections focus on Mitochondria morphometry in 3d datasets of mouse brain obtained with serial block-face Scanning Electron Microscopy, Serial Block-face Scanning Electron Microscopy of Schmidtea mediterranea, Correlative multiscale microCT-SBF-SEM imaging of resin-embedded tissue, Methods of enhanced FIB-SEM sample preparation and image acquisition, Functional characterization of endo-lysosomal compartments by correlative live-cell and volume electron microscopy, and much more.
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Table of Contents
1. Automated large volume sample preparation for vEMClaudia S. Lopez
2. Resin comparison for Serial Block Face Scanning Volume Electron Microscopy
Anneke Kremer, Saskia Lippens and Chris Geurin
3. Immunolabelling for SBF-SEM
Alice Liang
4. Volume Electron Microscopy in Plants
Kirk James Czymmek
5. Serial section electron tomography
Andrea Raimondi
6. Automated Tape Collecting Ultramicrotomy (ATUM) for targeting neuropathology
Martina Schifferer
7. Array tomography
Jemima J. Burden and Ian White
8. Mitochondria morphometry in 3d datasets of mouse brain obtained with serial block-face Scanning Electron Microscopy
Christel Genoud, Jean-Yves Chatton and Wei Jiao
9. Serial Block-face Scanning Electron Microscopy of Schmidtea mediterranea
Stephanie Nowotarski
10. Correlative multiscale microCT-SBF-SEM imaging of resin-embedded tissue
Rohan M. Lewis, Orestis L. Katsamenis and Pascale Chavatte-Palmer
11. Methods of enhanced FIB-SEM sample preparation and image acquisition
Song Pang
12. Functional characterization of endo-lysosomal compartments by correlative live-cell and volume electron microscopy
Nalan Liv
13. Cryo FIB-SEM imaging
Michele C. Darrow and James Naismith
14. Toward scalable reuse of vEM data: OME-Zarr to the rescue
Norman Rzepka, Joshua Allen Moore and John Bogovic
15. Public archiving of vEM data
Gerard J. Kleywegt and Ardan Patwardhan
