Fundamentals of Recombinant Protein Production, Purification and Characterization is organized into nine chapters in a logical fashion that cover an introduction to recombinant proteins and expression in different host expression systems, extraction, purification and analysis of proteins. This important reference features protocols, along with the advantages and disadvantage of each expression hosts and characterization technique (presented in tabular format) and offers detailed coverage of all aspects of protein production and processing (upstream and downstream processing) in one place. Finally, the book ends with different characterization techniques. Production of recombinant proteins for biotechnological and therapeutic applications at a large scale is an essential need of mankind. With the huge application potential of therapeutic and industrial proteins, there has been increasing demand for effective and efficient bioprocessing strategies. Recent progress around recombinant DNA technologies and bioprocessing strategies has paved the way for efficient production of recombinant proteins. Important factors such as insolubility and cost of production need to be considered for large scale production of these recombinant proteins.
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Table of Contents
1. Introduction to recombinant protein expression and purification2. Recombinant protein expression in Escherichia coli
3. Recombinant protein expression in Pichia pastoris
4. Mammalian cells for recombinant protein production via transient gene transfer
5. Expression of recombinant proteins in Chlamydomonas reinhardtii
6. Extraction and concentration of proteins (Laboratory scale)
7. Chromatographic and non-chromatographic techniques for purifying recombinant proteins
8. Analysis for purity of recombinant proteins
9. Characterization of recombinant proteins
Authors
Deepti Yadav Department of Biotechnology and Food Science, Faculty of Applied Sciences, Durban University of Technology, Durban, South Africa.Dr. Deepti Yadav grew up in Lucknow, Uttar Pradesh, India. After getting a master's degree in microbiology from Delhi University, she joined the Durban University of Technology in South Africa to pursue her PhD in biotechnology with Prof. Tukayi Kudanga's research group within the Department of Biotechnology and Food Science.
Dr. Yadav has worked in a number of research labs, where she gained hands-on experience in techniques such as molecular biology, tissue culture, cloning, protein expression in recombinant hosts and protein purification. She is particularly interested in the fields of enzyme biotechnology, enzyme kinetics, and enzyme applications.
After obtaining her PhD degree, Dr. Yadav was hired as a Research Associate by the School of Energy Materials at Mahatma Gandhi University, Kottayam, where she is working on synthesis of polyhydroxybutyrate from a locally available water hyacinth plant. She has written several scholarly papers and has been invited to speak at conferences. She has also coached and encouraged numerous young students to seek professions in biotechnology.
Abhishek Guldhe Associate Professor, Amity Institute of Biotechnology, Amity University, Maharashtra, India.Dr. Abhishek Guldhe is an Associate Professor at Amity Institute of Biotechnology, Amity University, Maharashtra, India. He was awarded with the prestigious Ramalingaswami Re-entry Fellowship of the Department of Biotechnology, Government of India. His research focus areas are microalgal biotechnology, biofuels, bioactive compounds, environmental biotechnology, and waste beneficiation. He teaches various subjects of biotechnology and environmental sciences. He has over 45 publications which include papers and book chapters in reputed international journals and books.
Tukayi Kudanga Professor, Department of Biotechnology and Food Science, Faculty of Applied Sciences, Durban University of Technology, Durban, South Africa.Prof. Tukayi Kudanga holds a PhD in biotechnology and is a professor in the Department of Biotechnology and Food Science at Durban University of Technology, South Africa. He is a recognized authority in laccase applications, particularly in biocatalysis and surface functionalization of lignocellulose materials. His areas of expertise include enzyme biotechnology, biocatalysis, and biomaterials. His current research focuses on enzymatic synthesis of bioactive compounds, enzymatic modification of food biopolymers, and development of green technologies for functionalization of lignocellulose materials. He is also interested in the search for novel biocatalysts, developing new applications and modifying enzymes for relevant biotechnological applications. Prof. Kudanga has extensive experience in teaching and research and has published his works widely. He has been invited to be plenary speaker at international conferences and reviews for several scientific journals.
