Table of Contents
- Characteristics of Sample Preparation for Electron Microscopy
- Cryo-FIB Developments to Enable Nanoscale Biopsies
- Preparing Bacterial Cells for Cryo-electron Tomography
- Revealing the Hidden World of Microbes in Situ: Sample Preparation Workflows for Cryo-electron Tomography
- Mastering the Preparation of Eukaryotic Cell Samples for Cryo-ET: Tips and Techniques
- Interpretation of Cellular Tomograms
- Denoising
- Feature Detection in cryo-Electron Tomography Image Analysis
- Validation
Authors
Dorit Hanein Department of Chemistry and Biochemistry and Department of Biological Engineering, University of California, Santa Barbara, CA, 93106, USA.Prof. Dorit Hanein received her doctoral degree from the Weizmann Institute in Israel. She completed her training as a Fulbright postdoctoral fellow at Brandeis University under the mentorship of Professor David DeRosier, a pioneer in three-dimensional image reconstruction techniques via electron microscopy. Prof. Hanein is affiliated with the University of California, Santa Barbara, and serves as a PEW Innovation Fund Investigator. She holds a "Habilitation � Diriger des Recherches� (HDR, Accreditation to Direct Research) from Sorbonne University, France.
Prof. Hanein's research lies at the intersection of structural biology, cell biology, systems biology, and engineering science. Her work focuses on the quantitative integration of high-resolution imaging technologies, designed to visualize the molecular architecture and dynamic conformational landscape of biological nanomachines in three dimensions within their native environments and under mechanical perturbations. She has made seminal contributions to our understanding of the cytoskeleton and macromolecular assemblies, advanced the field of quantitative electron microscopy, pioneered the use of correlative light and cryo-electron microscopic tomography and functionalized substrates. The strategic employment of these techniques has revolutionized our ability to define the building blocks of large dynamic macromolecular complexes in three dimensions with high fidelity and high resolution, while contextualizing their function within whole cells.
Niels Volkmann Department of Biological Engineering, Department of Electrical and Computer Engineering, Biomolecular Science and Engineering Program, University of California, Santa Barbara, CA, USA. Niels Volkmann earned his Ph.D. in Biophysics from the University of Hamburg, Germany in 1993, where he also trained as a postdoctoral fellow at the Max Planck Research Unit for Ribosome Structure. He received additional postdoctoral training at Brandeis University at the Rosenstiel Basic Medical Science Research Center and The Keck Center for Cellular Visualization prior to joining Sanford Burnham Prebys in 1999. Dr. Volkmann was promoted to the faculty as Assistant Professor in 2001.Dr. Volkmann's lab seeks to understand biological processes as integrated, hierarchical systems rather than as isolated parts, by developing and applying quantitative analysis tools to link and integrate the scales of atomic-resolution information and imaging of dynamic events in living cells. A key feature of Dr. Volkmann's research is the highly synergistic collaborations between his laboratory and other research groups (particularly Dr. Hanein's) that are addressing fundamental questions in cell and cancer biology.
