Steroid Biochemistry, Volume 688 in the Methods of Enzymology series, highlights new advances in the field, containing chapters on a variety of timely topics, including Cytochrome P450 Enzyme, Steroidogenic P450s (CYP11A1, 17A1, 21A2, 11B1, and 11B5), Steroid 17 alpha-hydroxylase/17,20-lyase (Cytochrome P450 17A1), Enzymes of Estrogen Biosynthesis, Aromatase and Steroid Sulfatase, Estrogenic 17b-Hydroxysteroid Dehydrogenase, Hydroxysteroid Dehydrogenases (HSD), 3a-Hydroxyssteroid Dehydrogenase, Approaches to Measuring 3b-Hydroxysteroid Dehydrogenase Type 1, 3b-Hydroxysteroid Dehydrogenase Type 2, and much more.
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Table of Contents
Section I. Cytochrome P450 Enzymes
1. Steroidogenic P450s (CYP11A1, 17A1, 21A2, 11B1, and 11B5)
Emily Scott
2. Steroid 17alpha-hydroxylase/17,20-lyase (Cytochrome P450 17A1)
F. Peter Guengerich, Yasuhiro Tateishi, Kevin D. McCarty and Lu Liu
Section 2. Enzymes of Estrogen Biosynthesis
3. Aromatase and Steroid Sulfatase
Debashi Ghosh
4. Estrogenic 17b-Hydroxysteroid Dehydrogenase
Section 3. Hydroxysteroid Dehydrogenases (HSD)
5. 3a-Hydroxyssteroid Dehydrogenase(s)
6. Approaches to Measuring 3b-Hydroxysteroid Dehydrogenase Type 1
Nima Sharifi
7. 3b-Hydroxysteroid Dehydrogenase Type 2
8. 11b-Hydroxysteroids Dehydrogenase Type 1
Alex Odermatt
9. 11b-Hydroxysteroids Dehydrogenase Type 2
Alex Odermatt
10. 17b-Hydroxysteroid Dehydrogenases
Tea Lanisnik Rizner
Section 4. Steroid Reductases
11. Approaches for evolutionary, biochemical, and structural analysis of bacterial steroid 5a-reductases
Ren Ruobing
12. Mammalian Steroid 5a-Reductase
Richard J. Auchus
13. Human Steroid 5b-Reductase (AKR1D1)
Trevor Martin Penning
14. Human Aldo-Keto Reductases (AKR1C1-AKR1C4)-Cloning & Expression of Recombinant Enzymes
Trevor Martin Penning and Andrea Detlefsen
Section 5. Steroid Conjugating Enzymes
15. Steroid sulfotransferases-X SULT2A1, 2B1b and 1E1
Charles Napoleon Falany
16. Steroid UGT-Glucuronsyl Transfereases
Section 6. Steroid Analytical Chemistry
17. Mass spectrometry of Glucocorticiids
18. Mass spectrometry of Progestins
19. LC-MS Quantitation of Hydroxy- and Keto-Androgens
Clementina Mesaros, Andrea Detlefsen and Ryan Paulukinas
20. Quantification of 11-oxygenated androgens by RP-UV-HPLC post 2,4-dinitrophenylhydrazine derivatization
Ryan Paulukinas
21. 11-Oxygenated Androgens
Amanda Swart
22. LC-MS Quantitation of Estrogens
Clementina Mesaros
