Steroid Biochemistry, Volume 688 in the Methods of Enzymology series, highlights new advances in the field, containing chapters on a variety of timely topics, including Cytochrome P450 Enzyme, Steroidogenic P450s (CYP11A1, 17A1, 21A2, 11B1, and 11B5), Steroid 17 alpha-hydroxylase/17,20-lyase (Cytochrome P450 17A1), Enzymes of Estrogen Biosynthesis, Aromatase and Steroid Sulfatase, Estrogenic 17b-Hydroxysteroid Dehydrogenase, Hydroxysteroid Dehydrogenases (HSD), 3a-Hydroxyssteroid Dehydrogenase, Approaches to Measuring 3b-Hydroxysteroid Dehydrogenase Type 1, 3b-Hydroxysteroid Dehydrogenase Type 2, and much more.
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Table of Contents
Section I. Cytochrome P450 Enzymes 1. Steroidogenic P450s (CYP11A1, 17A1, 21A2, 11B1, and 11B5) Emily Scott 2. Steroid 17alpha-hydroxylase/17,20-lyase (Cytochrome P450 17A1) F. Peter Guengerich, Yasuhiro Tateishi, Kevin D. McCarty and Lu Liu
Section 2. Enzymes of Estrogen Biosynthesis 3. Aromatase and Steroid Sulfatase Debashi Ghosh 4. Estrogenic 17b-Hydroxysteroid Dehydrogenase
Section 3. Hydroxysteroid Dehydrogenases (HSD) 5. 3a-Hydroxyssteroid Dehydrogenase(s) 6. Approaches to Measuring 3b-Hydroxysteroid Dehydrogenase Type 1 Nima Sharifi 7. 3b-Hydroxysteroid Dehydrogenase Type 2 8. 11b-Hydroxysteroids Dehydrogenase Type 1 Alex Odermatt 9. 11b-Hydroxysteroids Dehydrogenase Type 2 Alex Odermatt 10. 17b-Hydroxysteroid Dehydrogenases Tea Lanisnik Rizner
Section 4. Steroid Reductases 11. Approaches for evolutionary, biochemical, and structural analysis of bacterial steroid 5a-reductases Ren Ruobing 12. Mammalian Steroid 5a-Reductase Richard J. Auchus 13. Human Steroid 5b-Reductase (AKR1D1) Trevor Martin Penning 14. Human Aldo-Keto Reductases (AKR1C1-AKR1C4)-Cloning & Expression of Recombinant Enzymes Trevor Martin Penning and Andrea Detlefsen
Section 5. Steroid Conjugating Enzymes 15. Steroid sulfotransferases-X SULT2A1, 2B1b and 1E1 Charles Napoleon Falany 16. Steroid UGT-Glucuronsyl Transfereases
Section 6. Steroid Analytical Chemistry 17. Mass spectrometry of Glucocorticiids 18. Mass spectrometry of Progestins 19. LC-MS Quantitation of Hydroxy- and Keto-Androgens Clementina Mesaros, Andrea Detlefsen and Ryan Paulukinas 20. Quantification of 11-oxygenated androgens by RP-UV-HPLC post 2,4-dinitrophenylhydrazine derivatization Ryan Paulukinas 21. 11-Oxygenated Androgens Amanda Swart 22. LC-MS Quantitation of Estrogens Clementina Mesaros
