Celiac Disease, Volume 179 in the Methods in Cell Biology series is composed of extensive protocols about novel and widely used techniques for celiac disease research. Topics covered in this volume include Pepsin trypsin digested gliadin treatment of intestinal cells in vitro, Ex vivo Gliadin Stimulation of Intestinal Cells, Measuring intestinal permeability in celiac disease ex vivo using Ussing chambers, Intestinal permeability assessment using lactulose and mannitol, In vivo sensitization to gliadin by oral administration, Separation of epithelial and immune cells from intestinal biopsy samples, Isolation and study of dendritic cells and macrophages from human intestinal samples, Gnobiotic mouse models to study gluten sensitivity, and more.
Other sections in this new release include In vitro differentiation of macrophages from peripheral blood cells of celiac patients, Assessment of activated gut-homing CD8+ T cells in blood by flow cytometry during a three-day gluten challenge, Isolation, cryopreservation and thawing of peripheral blood mononuclear cells for downstream applications, and more.
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Table of Contents
1. Pepsin-trypsin digested gliadin treatment in intestinal cellsAne Olazagoitia-Garmendia
2. Ex vivo gliadin stimulation of intestinal cells
Linda Zhang and Chuan He
3. Measuring intestinal permeability in celiac disease ex vivo, using Ussing chambers
Olga Biskou and Amaia Jauregi-Miguel
4. Intestinal permeability assessment using lactulose and mannitol in celiac disease
Silvia Martinez Velasco, Azucena Gonzalez Garcia, Inaki Xarles Irastorza Terradillos, and
Jose Ramon Bilbao Catala
5. In vivo sensitization to gliadin by oral administration
M. Mar Romero, Dolors Serra, Ainara Castellanos-Rubio, and Laura Herrero
6. Separation of epithelial and immune cells from biopsy samples
Irati Romero-Garmendia
7. Study and isolation of human intestinal dendritic cell and macrophage subsets
Elisa Arribas-Rodriguez, Luis Fernandez-Salazar, Beatriz de Andres, Eduardo Arranz, Jose A. Garrote, and David Bernardo
8. Elucidating the role of microbes in celiac disease through gnotobiotic modeling
Alberto Caminero, Elena F. Verdu, and Heather J. Galipeau
9. In vitro differentiation of macrophages from peripheral blood cells of celiac patients
Federica Farina, Mariavittoria Laezza, Alessio Fasano, and Giovanna Del Pozzo
10. Assessment of activated gut-homing CD8+ T cells in blood by flow cytometry during a 3-day gluten challenge
Concepcion Nu�ez, Sara Gomez-Aguililla, Maria Corzo, F. Fernandez-Ba�ares, A. Bodas, S. Farrais,
N. Lopez-Palacios, and Mercedes Rubio
11. Isolation and cryopreservation of peripheral blood mononuclear cells
Sara Gomez-Aguililla, Concepcion Nu�ez, Mercedes Rubio, and Maria Corzo
12. Flow cytometric analysis of duodenal intraepithelial lymphocytes (celiac lymphogram): A diagnostic test for celiac disease
Concepcion Nu�ez, A. Carrasco, Maria Corzo, R. Pariente, M. Esteve, and G. Roy
13. The HLA-DQ8 transgenic mouse: A model to study the immune and cytotoxic responses to wheat gliadin
Francesco Maurano, Vera Rotondi Aufiero, Lucia Treppiccione, Stefano Rossi, Diomira Luongo, Giuseppe Mazzarella, and Mauro Rossi
14. Human intestinal organoid models for celiac disease research
Valeriia Dotsenko, Amir-Babak Sioofy-Khojine, Heikki Hyoty, and Keijo Viiri
15. Preparation of pepsin trypsin digested gliadin for stimulation experiments
Luis Manuel Mendoza-Gomez, Maialen Sebastian-delaCruz, Ane Olazagoitia-Garmendia, Itziar Gonzalez-Moro, Henar Rojas-Marquez, Jon Mentxaka, Izortze Santin, and Ainara Castellanos-Rubio
16. A fast, cheap, and easy protocol for celiac disease HLA haplotype typing using buccal swabs
Maialen Sebastian-delaCruz and Ainara Castellanos-Rubio
