Biochemistry, An Integrative Approach with Expanded Topics, offers a unique approach to the subject, emphasizing problem-solving and critical thinking over rote memorization. It integrates subjects of interest to undergraduates majoring in, biochemistry, life science, and beyond while preserving a chemical perspective. The book covers DNA, RNA, gene regulation, synthetic proteins, omics, plant biochemistry, and more. Key concepts such as metabolism, are introduced and then revisited and cross-referenced throughout the text to establish pattern recognition and help students commit their new knowledge to long-term memory. Emphasis is also laid on problem-solving and reasoning skills essential to life sciences, including data analysis and research assessment. This edition of the book aims to empower students studying a range of disciplines to develop a lasting foundation in biochemistry and metabolism.
Table of Contents
1 The Chemical Foundations of Biochemistry 1
2 Nucleic Acids 27
3 Proteins I: An Introduction to Protein Structure, Function and Purification 69
4 Proteins II: Enzymes, Allosterism and Receptor-Ligand Interactions 133
5 Membranes and an Introduction to Signal Transduction 193
6 Carbohydrates I: Mono- and Disaccharides, Glycolysis, Gluconeogenesis, and the Fates of Pyruvate 217
7 The Common Catabolic Pathway 269
8 Carbohydrates II 315
9 Lipids I: Fatty Acids, Steroids, and Eicosanoids; Beta-Oxidation and Fatty Acid Biosynthesis 363
10 Lipids II: Metabolism and Transport of Complex Lipids 415
11 Amino Acid and Amine Metabolism 461
12 Regulation and Integration of Metabolism 505
13 Nucleotide and Deoxynucleotide Metabolism 541
14 DNA Replication, Damage, and Repair 571
15 RNA Synthesis and Processing 611
16 Protein Biosynthesis 643
17 Control and Regulation of Gene Expression 673
18 Determination of Macromolecular Structure 715
19 Protein Production, Folding, and Engineering 745
20 Bioinformatics and Omics 775
21 Signal Transduction 801
22 Protein Trafficking 833
23 Photosynthesis and Nitrogen Fixation 853
TECHNIQUES T-1
SOLUTIONS A-1
GLOSSARY G-1
INDEX I-1
