An update to the popular guide to proteomics technology applications in biomedical research
Building on the strength of the original edition, this book presents the state of the art in the field of proteomics and offers students and scientists new tools and techniques to advance their own research. Written by leading experts in the field, it provides readers with an understanding of new and emerging directions for proteomics research and applications.
Proteomics for Biological Discovery begins by discussing the emergence of proteomics technologies and summarizing the potential insights to be gained from proteome-level research. The tools of proteomics, from conventional to novel techniques, are thoroughly covered, from underlying concepts to limitations and future directions. Later chapters provide an overview of the current developments in post-translational modification studies, structural proteomics, biochemical proteomics, applied proteomics, and bioinformatics relevant to proteomics. Chapters cover: Quantitative Proteomics for Differential Protein Expression Profiling; Protein Microarrays; Protein Biomarker Discovery; Biomarker Discovery using Mass Spectrometry Imaging; Protein-Protein Interactions; Mass Spectrometry Of Intact Protein Complexes; Crosslinking Applications in Structural Proteomics; Functional Proteomics; High Resolution Interrogation of Biological Systems via Mass Cytometry; Characterization of Drug-Protein Interactions by Chemoproteomics; Phosphorylation; Large-Scale Phosphoproteomics; and Probing Glycoforms of Individual Proteins Using Antibody-Lectin Sandwich Arrays.
- Presents a comprehensive and coherent review of the major issues in proteomic technology development, bioinformatics, strategic approaches, and applications
- Chapters offer a rigorous overview with summary of limitations, emerging approaches, questions, and realistic future industry and basic science applications
- Features new coverage of mass spectrometry for high throughput proteomic measurements, and novel quantitation strategies such as spectral counting and stable isotope labeling
- Discusses higher level integrative aspects, including technical challenges and applications for drug discovery
- Offers new chapters on biomarker discovery, global phosphorylation analysis, proteomic profiling using antibodies, and single cell mass spectrometry
Proteomics for Biological Discovery is an excellent advanced resource for graduate students, postdoctoral fellows, and scientists across all the major fields of biomedical science.
Table of Contents
Foreword vii
List of Contributors ix
1. Quantitative Proteomics for Differential Protein Expression Profiling 1
Christian K. Frese, Henk van den Toorn, Albert J.R. Heck, and Shabaz Mohammed
2. Protein Microarrays 29
Fernanda Festa and Joshua LaBaer
3. Protein Biomarker Discovery: An Integrated Concept 63
Andrei P. Drabovich, Eduardo Martinez‐Morillo, and Eleftherios P. Diamandis
4. Biomarker Discovery with Mass Spectrometry Imaging and Profiling 89
Sage J.B. Dunham, Elizabeth K. Neumann, Eric J. Lanni, Ta-Hsuan Ong, and Jonathan V. Sweedler
5. Protein-Protein Interactions 125
Claire M. Delahunty and John R. Yates III
6. Mass Spectrometry of Intact Protein Complexes 145
Jonathan T.S. Hopper and Carol V. Robinson
7. Cross‐linking Applications in Structural Proteomics 175
Evgeniy V. Petrotchenko, Jason J. Serpa, and Christoph H. Borchers
8. Functional Proteomics: Systematic Characterization of the Physical and Functional Organization of Cell Systems 197
Pierre C. Havugimana, Pingzhao Hu, and Andrew Emili
9. High‐Resolution Interrogation of Biological Systems via Mass Cytometry 215
Heather M. Grundhofer, Michelle M. Kuhns, and Edgar A. Arriaga
10. Characterization of Drug-Protein Interactions by Chemoproteomics 247
Markus Schirle, Marcus Bantscheff, and Bernhard Kuster
11. Phosphorylation 265
Timothy D. Veenstra
12. Large‐Scale Phosphoproteomics 291
John R. Yates III
13. Probing Glycoforms of Individual Proteins Using Antibody‐Lectin Sandwich Arrays: Methods and Findings from Studies of Pancreatic Cancer 311
Brian B. Haab
Index 329
