A powerful and essential resource for researchers with an interest in CE-MS
In Capillary Electrophoresis - Mass Spectrometry for Proteomics and Metabolomics: Principles and Applications, a team of distinguished researchers delivers a comprehensive overview of bioanalytical capillary electrophoresis coupled to mass spectrometry (CE-MS). The book explains foundational principles, technology as well the strategies and techniques used in data analysis for metabolic and proteomic studies. It also provides a global overview of recent developments and advances for improving CE-MS sensitivity and reproducibility.
An essential handbook for everyone performing metabolomic and proteomic analysis, the information provided here will assist researchers in tapping into the full potential of this technique to answer biological and clinical questions.
Readers will also find: - A thorough introduction to the principles of capillary electrophoresis, including its fundamentals, CE separation modes, capillary coatings, and the fundamentals of mass spectrometry - In-depth examinations of technological developments in capillary electrophoresis, including sample preparation, online preconcentration, detection sensitivity, and metabolic coverage - Comprehensive discussions of metabolomic studies, including their biomedical and clinical applications - Recent advances in proteomics, including top-down and bottom-up approaches
Perfect for analytical and clinical chemists, Capillary Electrophoresis - Mass Spectrometry for Proteomics and Metabolomics: Principles and Applications will also earn a place in the libraries of biochemists, molecular biologists, and other molecular life scientists.
Table of Contents
1. Capillary Electrophoresis-Mass Spectrometry Interfacing: Principles and Recent Developments
2. Data Analysis Strategies in CE-MS for Metabolomics
3. Data Processing Workflow for Relative Quantification from Label-Free and Isobaric Labeling-Based Untargeted Shotgun Proteomics: From Database Search to Differential Expression Analysis
4. Data Processing in Metabolomics Capillary Electrophoresis-Mass Spectrometry
5. Utility and Advances of Capillary Electrophoresis-Mass Spectrometry for Metabolomics
6. Comprehensive Lipid Profiling by Multisegment Injection-Nonaqueous Capillary Electrophoresis-Mass Spectrometry: Expanding Coverage Beyond Hydrophilic Metabolites
7. Strategies for Identification of Modified Amino Acids with CE-MS in Metabolomics
8. CE-MS Approaches for Single-Cell Metabolomics
9. CE-MS Approaches for Peptidomics
10. Capillary Zone Electrophoresis-Mass Spectrometry for Top-Down Proteomics: Technological Development and Biological Applications
11. CE-MS Methods for the Characterization of Monoclonal Antibodies
12. CE and CE-MS Approaches for Glycan Analysis
13. CE-MS Approaches for Glyco(proteo)mic Analysis
