All-in-one guide to successful lipidomic analysis, combining the latest advances and best practices from academia, industry, and clinical research
Mass Spectrometry for Lipidomics presents a systematic overview of lipidomic analysis, covering established standards of lipid analysis, available technology, and key lipid classes, as well as applications in basic research, medicine, pharma, and the food industry.
Through connecting recent technological advances with key application areas, this unique guide bridges the gap between academia and industry by translating the vast body of knowledge that has been gained in the past decade into much-needed practical advice for novices as well as routine users.
Edited by the president and vice-president of the International Lipidomics Society with contributions from the top experts in lipid analysis, Mass Spectrometry for Lipidomics covers a wide range of key topics, including: - Aspects of sample preparation, separation methods, different mass spectrometry modes, as well as identification and quantitation, including the use of bioinformatics tools for data analysis - Identification, quantitation and profiling of lipids in different types of biological samples - Analytical approaches for all major classes of biological lipids, from fatty acids to phospholipids to sterols - Novel applications in biological research, clinical diagnostics, as well as food and crop science
For analytical chemists, biochemists, clinical chemists, and analytical laboratories and hospitals, Mass Spectrometry for Lipidomics presents a comprehensive and authoritative overview of the subject, with unmatched expertise from practicing professionals actively involved in the latest research.
Table of Contents
Preface xiii
1 Introduction to Lipidomics 1
Harald C. Köfeler, Kim Ekroos, and Michal Holcapek
Part I Analytical Methodologies in Lipidomics 13
2 Preanalytics for Lipidomics Analysis 15
Gonçalo Vale and Jeffrey G. McDonald
3 Direct Infusion (Shotgun) Electrospray Mass Spectrometry 41
Marcus Höring and Gerhard Liebisch
4 Liquid Chromatography - and Supercritical Fluid Chromatography - Mass Spectrometry 91
Michal Holcapek, Ondrej Peterka, Michaela Chocholousková, and Denise Wolrab
5 Mass Spectrometry Imaging of Lipids 117
Shane R. Ellis and Jens Soltwisch
6 Ion Mobility Spectrometry 151
Kaylie I. Kirkwood, Melanie T. Odenkirk, and Erin S. Baker
7 Structural Characterization of Lipids Using Advanced Mass Spectrometry Approaches 183
Josef Cvacka, Vladimír Vrkoslav, and stepán Strnad
8 Lipidomic Identification 227
Harald Köfeler
9 Lipidomics Quantitation 255
Michaela Chocholousková, Denise Wolrab, Ondrej Peterka, Robert Jirásko, and Michal Holcapek
10 The Past and Future of Lipidomics Bioinformatics 271
Dominik Kopczynski, Daniel Krause, Fadi Al Machot, Dominik Schwudke, Nils Hoffmann, and Robert Ahrends
Part II Lipidomic Analysis According to Lipid Categories and Classes 291
11 Fatty Acids: Structural and Quantitative Analysis 293
Dong Hao Wang and J. Thomas Brenna
12 Quantitation of Oxylipins in Biological Samples, Focusing on Plasma, and Urine 317
Valerie B. O'Donnell, Ginger L. Milne, Marina S. Nogueira, Martin Giera, and Nils Helge Schebb
13 Mass Spectrometry for Analysis of Glycerolipids 351
Wm. Craig Byrdwell
14 Lipidomic Analysis of Glycerophospholipid Molecular Species in Biological Samples 395
Xianlin Han
15 Sphingolipids 425
Lukas Opalka, Lisa Schlicker, and Roger Sandhoff
16 Sterol Lipids 481
William J. Griffiths, Eylan Yutuc, and Yuqin Wang
17 Bile Acids 509
Sebastian Simstich and Günter Fauler
Part III Lipidomic Applications 531
18 Lipidomic Profiling in a Large-Scale Cohort 533
Daisuke Saigusa
19 Cancer Lipidomics - From the Perspective of Analytical Chemists 545
Denise Wolrab, Ondrej Peterka, Michaela Chocholousková, and Michal Holcapek
20 Lipidomics in Clinical Diagnostics 557
Jayashree Selvalatchmanan, Markus R. Wenk, and Anne K. Bendt
21 Lipidomics in Food Industry and Nutrition 585
Danilo Donnarumma, Giuseppe Micalizzi, Luigi Mondello, and Paola Dugo
22 Lipidomics in Plant Science 601
Zoong Lwe Zolian, Yu Song, P. A. D. B. Vinusha Wickramasinghe, and Ruth Welti
23 Lipidomics in Multi-Omics Studies 625
Bjoern Titz, Oksana Lavrynenko, and Nikolai V. Ivanov
24 Tracer Lipidomics 641
Jonas Dehairs, Ine Koeken, Lake-Ee Quek, Andrew Hoy, Bart Ghesquière, and Johannes V. Swinnen
25 Mass Spectrometry for Lipidomics: Methods and Applications - Aging and Alzheimer's Disease 657
Kevin Huynh, Habtamu B. Beyene, Tingting Wang, Corey Giles, and Peter J. Meikle
26 Lipidomics in Cell Biology 669
Noemi Jiménez-Rojo, Fabrizio Vacca, and Howard Riezman
27 Microbial Lipidomics 689
Masahiro Ueda, Nobuyuki Okahashi, and Makoto Arita
Index 705
