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Detection and Analysis of SARS Coronavirus. Advanced Biosensors for Pandemic Viruses and Related Pathogens. Edition No. 1

  • Book

  • 304 Pages
  • August 2021
  • John Wiley and Sons Ltd
  • ID: 5838591
Detection and Analysis of SARS Coronavirus

Detecting and analyzing the COVID-19 pandemic with biosensor technology

The highly contagious SARS CoV-2 pathogen has challenged health systems around the world as they struggle to detect and monitor the spread of the pathogen. In Detection and Analysis of SARS Coronavirus: Advanced Biosensors for Pandemic Viruses and Related Pathogens expert chemists Chaudhery Mustansar Hussain and Sudheesh K. Shukla deliver a practical analysis of how contactless coronavirus detectors may be developed using existing biosensor technology.

The editors outline current challenges in the field, the bioanalytical principles for coronavirus detection, and available biosensor technology. They then move on to how available technology might be adapted to detect coronaviruses and how commercialization of the technology might unfold.

The lessons learned in this book are readily applicable to the study of other current and emerging pathogens.

Readers will also enjoy: - A thorough introduction to the current diagnostic approaches for COVID-19, including common challenges, technology adaptation, and future potential - An exploration of bio-analytical strategies for SARS CoV-2/COVID-19, including COVID detection via nanotechnology, biosensing approaches, and the role of nanotechnology in coronavirus detection - Practical discussions of biosensors for the analysis of SARS CoV-2/COVID-19, including sensor development for coronavirus and chemical sensors for coronavirus diagnosis - In-depth treatments of the commercialization and standardization for analytical technologies

Perfect for virologists, pharmaceutical industry professionals, and sensor developers, Detection and Analysis of SARS Coronavirus is also an indispensable resource for those working in analytical research institutes, biotechnology industry professionals, and public health agency professionals.

Table of Contents

Preface xv

About the Editors xvii

Part I Introduction 1

1 Current Diagnostic Approach for COVID-19 3
Nitika Thakur and Rachit Sood

1.1 Introduction 3

1.2 Recommended Laboratory Diagnosis for COVID-19 3

1.2.1 SARS-CoV-2 Testing: Detection Approach by Screening Suitable Specimen Cultures 3

1.2.2 SARS-CoV-2 Detection: The Nucleic Acid Approach 4

1.2.2.1 COVID-19 Detection Approach Through Real-Time PCR 4

1.2.2.2 Detection Approach Through Nested RT-PCR 5

1.2.2.3 Detection and Analysis Approach via Droplet Digital PCR 6

1.2.2.4 Lab-on-chip Approaches Using Nucleic Acid as Chief Target Points 6

1.2.2.5 Analysis Through Nanoparticle Amplification Process 7

1.2.2.6 Portable Methodology: The Concept of Benchtop-Sized Analyzer 7

1.3 Antigenic Approach for COVID-19 Diagnosis 8

1.4 Antibody Diagnostic Strategies for Detection of COVID-19 10

1.4.1 Enzyme-Linked Immunosorbent Strategies: The Vircell and Euroimmun ELISA 11

1.4.2 Immunoassay-Based Detection Approach: Immunofluorescence and Chemiluminescence Assay 11

1.5 Point-of-care/Lab-on-chip Approaches: The LFA (Lateral Flow Assay) 12

1.6 Miniaturization Detection Approach: Combining Microarray with Microfluidic Chip Technology 12

1.7 Neutralization Detection Approaches Toward COVID-19 13

1.8 Genomic Sequencing Detection Approach: The Amplicon, Hybrid Capture, and Meta-transcriptomic Strategy 13

1.9 Conclusion 14

References 14

2 COVID-19 Diagnostics: Current Approach, Challenges, and Technology Adaptation 23
Prama Bhattacherjee, Santanu Patra, Abhishek Mishra, Trupti R. Das, Hemlata Dewangan, Rajgourab Ghosh, Sudheesh K. Shukla, and Anshuman Mishra

2.1 Introduction 23

2.2 Diagnosis of COVID-19 25

2.2.1 Clinical Diagnosis 25

2.2.2 Sample Collection and Testing 26

2.3 Understanding Genetic Consequences 27

2.3.1 SARS-CoV-2 Genome and Database 27

2.3.2 Infection and Genetic Diagnosis 27

2.3.3 Real-Time PCR 27

2.4 Understanding Immunological Consequences 28

2.4.1 Role of Immunological Test 28

2.4.2 Rapid Antigen Testing 29

2.4.3 Rapid Antibody Tests 29

2.5 Protein Testing 29

2.5.1 Computed Tomography 29

2.6 Challenges 30

2.6.1 Challenges of Developing COVID-19 Tests 30

2.6.2 Sample Collection and Tests 31

2.7 Advanced Diagnosis Technologies and Adaptation 31

2.8 Adaptation of a New Approach 31

2.8.1 Emerging Diagnostic Tests for COVID-19 33

2.8.2 Role of siRNA, Nanoparticle Toward COVID-19 33

2.8.3 RT-LAMP Nucleic Acid Testing 34

2.8.4 Point-of-care Testing 34

2.8.5 FNCAS9 Editor-Limited Uniform Detection Assay 34

2.8.6 Development of a Novel Technology for COVID-19 Rapid Test 34

2.8.7 Specific High-Sensitivity Enzymatic Reporter Unlocking 35

2.9 Digital Healthcare Technologies 35

2.9.1 Artificial Intelligence and Mass Healthcare 36

2.9.2 Standard Healthcare Management During Pandemic Crisis 36

2.10 Implications of Technology-Based Diagnosis and Testing 36

2.10.1 Benefit of Diagnosis 37

2.11 Conclusion 37

2.12 Future Prospects 38

Acknowledgment 39

References 39

3 Current Scenario of Pandemic COVID-19: Overview, Diagnosis, and Future Prospective 43
Bindu Mangla, Shinu Chauhan, Shreya Kathuria, Prashant, Mohit, Meenakshi, Santanu Patra, Sudheesh K. Shukla, and Chaudhery Mustansar Hussain

3.1 Introduction 43

3.2 Diagnosis and Treatment 47

3.3 Infection and Control 49

3.4 Current Status of COVID-19 50

3.5 Recommendation 51

3.6 Conclusion 52

References 53

Part II Bio-analytical Strategies for SARS-CoV-2/COVID-19 57

4 COVID Detection via Nanotechnology: A Promising Field in the Diagnosis and Analysis of Novel Coronavirus Infection 59
Nitika Thakur, Sudheesh K. Shukla, and Chaudhery M. Hussain

4.1 Introduction 59

4.1.1 Pandemic Outbreak of COVID-19: A Tour Around the Globe from Wuhan 59

4.1.2 Nanotech Solutions for Faster Detection Analysis of COVID-19 60

4.2 Methodologies from Lab to People: Advantages of Nanovaccines in Providing Point-of-care Diagnosis 60

4.3 An Overview: The Potential Strategies Related to Nanotechnology for Combating COVID-19 61

4.3.1 Loop-Mediated Isothermal Reverse Transcriptase Coupling with Nanobiosensors 62

4.3.2 Nanopoint-of-care/Lab-on-chip Diagnosis: A Strategy to Reach out the Resource-Poor Areas 63

4.3.3 Tagging up the Biosensor with Optics for Reducing the Long Detection Time 63

4.3.4 Sequencing Strategy Involving the Nanopore-Assisted Target Sequencing (NTS) 63

4.4 Screening of Potential Agents for Restricting the Rapid Spread of COVID-19 64

4.5 Potential New Generation Vaccines: A Journey from Nucleoside, Subunit, Peptide Analogs to Nanoformulation 65

4.5.1 Nucleoside Analog Vaccines: Searching Potential Candidates Among DNA, RNA, and mRNA 65

4.5.2 Nano-VLP Subunit Vaccines: A Stable and Ordered Vaccine Complex 67

4.5.3 Nanopeptide-Based Vaccines: “Hitchhiking Through Albumin” 68

4.6 Future Prospective: Resolving the Big Pandemics 68

4.7 Conclusion 69

References 69

5 Biosensing Approach for SARS-CoV-2 Detection 75
Varun Rawat, Sonam, Diksha Gahlot, Kritika Nagpal, and Seema R. Pathak

5.1 Introduction 75

5.2 SARS-COVID-19 Structure and Genome 76

5.3 SARS-COVID-19 Sensors 77

5.3.1 Localized Surface Plasmon Resonance (LSPR) Sensor 77

5.3.2 Field Effect Transistor (FET) 78

5.3.3 Cell-Based Potentiometric Biosensor 79

5.3.4 eCovSens 79

5.3.5 CRISPR/Cas12 80

5.3.6 DNA Nanoscaffold Hybrid Chain Reaction (DNHCR)-Based Fluorescence Biosensor 81

5.4 Biomarkers 83

5.5 Conclusion 84

References 84

6 Role of Nanotechnology in Coronavirus Detection 87
AbdulGafar O. Tiamiyu, Bashir Adelodun, Hashim O. Bakare, Fidelis O. Ajibade, Kola Y. Kareem, Rahmat G. Ibrahim, Golden Odey, Madhumita Goala, and Jamiu A. Adeniran

6.1 Introduction 87

6.2 Application of Nanomaterials 88

6.2.1 Silver Nanoparticles 88

6.2.2 Gold Nanoparticles 88

6.2.3 Carbon Nanotubes 89

6.3 Nanotechnology and Application in Medicine 90

6.3.1 Biobarriers 90

6.3.2 Molecular Imaging 90

6.3.3 Early Detection 91

6.3.4 Nanodiagnostics 91

6.4 Biosensors for Infectious Disease Detection 92

6.4.1 Biosensors 93

6.4.2 Nano-Based Biosensors 93

6.5 Coronavirus Detection 93

6.5.1 Biosensors for COVID-19 Detection 94

6.5.2 Nano-Based Biosensors for Coronavirus Detection 95

6.6 Emerging Concerns on COVID-19 96

6.6.1 Nanotechnology in COVID-19 ContaminatedWater 97

6.7 Nanotoxicity 98

6.8 Conclusion 98

References 99

Part III Biosensors for Analysis of SARS-CoV-2/COVID-19 105

7 Sensor Development for Coronavirus 107
Ranjita D. Tandel, Nagappa L. Teradal, and Sudheesh K. Shukla

7.1 Introduction 107

7.2 Conclusions 118

7.3 Future Perspectives 119

References 119

8 Chemical Sensor for the Diagnosis of Coronavirus 123
Gyandshwar K. Rao, Ashish K. Sengar, and Seema R. Pathak

8.1 Introduction 123

8.2 Multiplexed Nanomaterial-Based Sensor 124

8.3 Nanomaterial-Mediated Paper-Based Sensors 126

8.4 Molecularly Imprinted Polymer-Based Technology 127

8.5 Dual-Functional Plasmonic Photothermal Sensors for SARS-CoV-2 Detection 128

8.6 Zirconium Quantum Dot-Based Chemical Sensors 128

8.7 Calixarene-Functionalized Graphene Oxide-Based Sensors 129

8.8 AlGaN/GaN High Electron Mobility Transistor-Based Sensors 130

8.9 Conclusion 132

References 132

9 Lab on a Paper-Based Device for Coronavirus Biosensing 137
Lucas Felipe de Lima, Ariana de Souza Moraes, Paulo de Tarso Garcia, and William Reis de Araujo

9.1 Paper-Based Technology as Point-of-care Testing Devices 137

9.1.1 Fabrication Methods 140

9.1.2 Main Detection Methods Coupled to PADs 141

9.2 Current Outbreak and Coronavirus Biology 142

9.3 Main Approach Used to COVID-19 Biosensing 144

9.4 Paper-Based Analytical Devices for COVID-19 Diagnostics 145

9.5 Challenges and Perspectives 155

Acknowledgments 156

References 157

Part IV Commercialization and Standardization of Analytical Technologies 163

10 Nanobioengineering Approach for Early Detection of SARS-CoV-2 165
Sidra Rashid, Umay Amara, Khalid Mahmood, Mian H. Nawaz, and Akhtar Hayat

10.1 Introduction 165

10.2 Can Nanobioengineering Stand in the Battle Against SARS-CoV-2? 166

10.3 Sequential and Molecular Data Analysis 167

10.3.1 Role of Nanobioengineering for SARS-CoV-2 Detection 168

10.4 Nanobioengineering-Based Detection of SARS-CoV-2 169

10.4.1 Nucleic Acid-Based Molecular Detection 169

10.4.1.1 Reverse Transcription Polymerase Chain Reaction (RT-PCR) 169

10.4.1.2 Loop-Mediated Isothermal Amplification (LAMP) 172

10.4.2 Protein-Based Detection 172

10.4.3 Lymphopenia-Based Assessment 175

10.4.4 Bioengineered Surfaces for SARS-CoV-2 Detection 177

10.4.5 Nanobioengineered Prototypes 177

10.4.6 Digital Radiographical Biosensing Platforms 177

10.4.7 Other Methods for SARS-CoV-2 Detection 179

10.5 Discussion 179

10.6 Conclusions 180

10.7 Expert Opinion 180

10.8 Future Directions 181

References 181

11 Development of Electrochemical Biosensors for Coronavirus Detection 187
Fulden Ulucan-Karnak, Cansu ˙I. Kuru, and Zeynep Yilmaz-Sercinoglu

11.1 Introduction 187

11.2 Detection of Viral Infections 187

11.2.1 Detection of Virus 187

11.2.1.1 Electron Microscopy 187

11.2.1.2 Viral Culture 188

11.2.2 Detection of Viral DNA/RNA 188

11.2.2.1 Real-Time Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) 188

11.2.2.2 Microarrays 189

11.2.3 Detection of Post-infection Antibodies 189

11.2.3.1 Lateral Flow Immunoassays (LFIAs) 190

11.2.3.2 Enzyme-Linked Immunosorbent Assay (ELISA) 190

11.2.3.3 Chemiluminescent Immunoassay (CLIA) 191

11.3 Current Biosensor Candidates for COVID-19 Detection 193

11.3.1 Electrochemical Biosensors for SARS-CoV-2 Detection 193

11.3.1.1 Impedimetry 195

11.3.1.2 Potentiometry 196

11.3.1.3 Conductometry 197

11.3.1.4 Voltammetry 197

11.3.1.5 Amperometry 198

11.4 Conclusions 199

References 201

12 Electrochemical Biosensor Fabrication for Coronavirus Testing 207
Monika Vats, Parvin, Mukul Taliyan, and Seema Rani Pathak

12.1 Introduction 207

12.2 Application of Electrochemical Biosensors 209

12.3 Fabrication of Electrochemical Biosensors 210

12.4 Fabrication of Electrochemical Biosensors for COVID-19 (Immunosensors) 212

12.5 Conclusion 214

References 215

Part V Outlook 219

13 Effects of COVID-19: An Environmental Point of View 221
Kola Y. Kareem, Bashir Adelodun, AbdulGafar O. Tiamiyu, Fidelis O. Ajibade, Rahmat G. Ibrahim, Golden Odey, Madhumita Goala, Hashim O. Bakare, and Jamiu A. Adeniran

13.1 Introduction 221

13.2 Methodological Approach 224

13.3 Effects of COVID-19 on Socioeconomic Development in the Environment 225

13.4 Environmental Management as an Important Factor for COVID-19 Transmission 225

13.5 Environmental Impact Assessment of COVID-19 226

13.5.1 Environmental Variables Related to COVID-19 226

13.5.2 Effects of COVID-19 on Global Physical Environment: Air Quality and Environmental Pollution 228

13.5.3 COVID-19 Impacts onWater Resources and Aquatic Life 231

13.5.4 COVID-19 Impacts on Ecological Parameters and Soil Systems 233

13.5.5 COVID-19 Impacts on Noise Pollution, Increased SolidWastes, and Recycling 234

13.5.6 COVID-19 Impacts onWastewater Quality and Sanitary Systems 234

13.5.7 Socioeconomic Environmental Impacts of COVID-19 235

13.5.8 Indirect Effects of COVID-19 on the Environment 235

13.6 Conclusion 236

References 237

14 COVID-19 Pandemic and CO2 Emission in the United States: A Sectoral Analysis 243
Afees A. Salisu, Tirimisiyu F. Oloko, and Idris A. Adediran

14.1 Introduction 243

14.2 Stylized Facts on the Effect of COVID-19 Pandemic on Sectoral CO2 Emission 245

14.3 Data Issues and Methodology 249

14.4 Empirical Results 251

14.4.1 Preliminary Results 251

14.4.2 Main Results 251

14.5 Conclusion 255

References 257

15 Theranostic Approach for Coronavirus 261
Anushree Pandey, Asif Ali, and Yuvraj S. Negi

15.1 Introduction 261

15.2 Conventional Medicines 262

15.3 Role of Nanoparticles in COVID-19 Detection 265

15.4 Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) Coupled with a Nanoparticle-Based Biosensor (NBS) Assay 265

15.5 Point-of-care Testing 266

15.6 Optical Biosensor Nanotechnology 268

15.7 Nanopore Target Sequencing (NTS) 268

15.8 Role of Nanotechnology in the Treatment 269

15.9 Conclusion 270

References 270

Index 275

Authors

Chaudhery Mustansar Hussain New Jersey Institute of Technology (NJIT), USA. Sudheesh K. Shukla University of Johannesburg, South Africa.