In recent years, significant advancements have been made in the management of nutritional deficiency using genome engineering - enriching the nutritional properties of agricultural and horticultural crop plants such as wheat, rice, potatoes, grapes, and bananas. To meet the demands of the rapidly growing world population, researchers are developing a range of new genome engineering tools and strategies, from increasing the nutraceuticals in cereals and fruits, to decreasing the anti-nutrients in crop plants to improve the bioavailability of minerals and vitamins.
Genome Engineering for Crop Improvement provides an up-to-date view of the use of genome editing for crop bio-fortification, improved bioavailability of minerals and nutrients, and enhanced hypo-allergenicity and hypo-immunogenicity. This volume examines a diversity of important topics including mineral and nutrient localization, metabolic engineering of carotenoids and flavonoids, genome engineering of zero calorie potatoes and allergen-free grains, engineering for stress resistance in crop plants, and more. Helping readers deepen their knowledge of the application of genome engineering in crop improvement, this book:
- Presents genetic engineering methods for developing edible oil crops, mineral translocation in grains, increased flavonoids in tomatoes, and cereals with enriched iron bioavailability
- Describes current genome engineering methods and the distribution of nutritional and mineral composition in important crop plants
- Offers perspectives on emerging technologies and the future of genome engineering in agriculture
Genome Engineering for Crop Improvement is an essential resource for academics, scientists, researchers, agriculturalists, and students of plant molecular biology, system biology, plant biotechnology, and functional genomics.
Table of Contents
List of Contributors x
Preface xvii
About the Editor xix
Acknowledgments xxi
1 An Overview of Genome-Engineering Methods 1
Sushmita, Gurminder Kaur, Santosh Kumar Upadhyay, and Praveen Chandra Verma
2 Distribution of Nutritional and Mineral Components in Important Crop Plants 22
Katarina Vogel-Mikuš, Paula Pongrac, Ivan Kreft, Primo Pelicon, Primo Vavpetič, Boštjan Jenčič, Johannes Teun van Elteren, Peter Kump, Sudhir P. Singh, and Marjana Regvar
3 Application of Genome Engineering Methods for Quality Improvement in Important Crops 43
Sajid Fiaz, Sher Aslam Khan, Galal Bakr Anis, Habib Ali, Mohsin Ali, Kazim Ali, Mehmood Ali Noor, Sibtain Ahmad, and Bilal Ahmad Asad
4 Genome Engineering for Enriching Fe and Zn in Rice Grain and Increasing Micronutrient Bioavailability 69
Conrado Dueñas, Jr., Gela Myan B. Mota, Norman Oliva, and Inez H. Slamet-Loedin
5 Development of Carotenoids Rich Grains by Genome Engineering 83
Vikrant Gautam, Gurwinder Singh Rana, Pankaj Kumar, and Santosh Watpade
6 CRISPR-Cas9 System for Agriculture Crop Improvement 97
Ashish Sharma, Poorwa Kamal Badola, and Prabodh Kumar Trivedi
7 Contribution of Crop Biofortification in Mitigating Vitamin Deficiency Globally 112
Siddhant Chaturvedi, Roni Chaudhary, and Siddharth Tiwari
8 Genome Editing Approaches for Trait Improvement in the Hairy Root Cultures of the Economically Important Plants 131
Pravin Prakash, Rakesh Srivastava, Ajay Kumar, Gurminder Kaur, and Praveen Chandra Verma
9 Phytic Acid Reduction in Cereal Grains by Genome Engineering: Potential Targets to Achieve Low Phytate Wheat 146
Ajay K. Pandey, Sipla Aggarwal, Varsha Meena, and Anil Kumar
10 Genome Engineering for Nutritional Improvement in Pulses 157
Chirag Uppal, Ajinder Kaur, and Chhaya Sharma
11 The Survey of Genetic Engineering Approaches for Oil/Fatty Acid Content Improvement in Oilseed Crops 181
Kaushal Kumar Bhati, Riyazuddin Riyazuddin, Ashish Kumar Pathak, and Anuradha Singh
12 Genome-Editing Mediated Improvement of Biotic Tolerance in Crop Plants 199
Krishan Mohan Rai and Harpal Singh
13 Genome Engineering and Essential Mineral Enrichment of Crops 210
Erum Shoeb, Uzma Badar, Srividhya Venkataraman, Ghyda Murad Hashim, and Kathleen Hefferon
14 Genome Editing to Develop Disease Resistance in Crops 224
Kashaf Zafar, Azka Noureen, Muhammad Jawad Akbar Awan, Naveed Anjum, Muhammad Qasim Aslam, Muhammad Zuhaib Khan, Imran Amin, and Shahid Mansoor
15 Biotechnological Approaches for Nutritional Improvement in Potato (Solanum tuberosum L.) 253
Chandrama Prakash Upadhyaya and Deepak Singh Bagri
16 Genome Engineering Strategies for Quality Improvement in Tomato 281
Tian Wang, Hongyan Zhang, and Hongliang Zhu
17 Genome Editing for Biofortification of Rice: Current Implications and Future Aspects 297
Suchismita Roy and Praveen Soni
18 Genome Editing for Improving Abiotic Stress Tolerance in Rice 314
Shweta Roy, Nirbhay Kumar Kushwaha, Hasthi Ram, and Praveen Soni
19 Role of Genome Engineering for the Development of Resistant Starch-Rich, Allergen-Free and Processing Quality Improved Cereal Crops 333
Anuradha Singh, Amit Yadav, Joy K. Roy, and Kaushal Kumar Bhati
20 Engineering of Plant Metabolic Pathway for Nutritional Improvement: Recent Advances and Challenges 351
Sameer Dixit, Akanchha Shukla, Vinayak Singh, and Santosh Kumar Upadhyay
21 Genome Engineering for Food Security 380
Sajid Fiaz, Sher Aslam Khan, Mehmood Ali Noor, Afifa Younas, Habib Ali, Kazim Ali, Mahmoud Mohamed Gaballah, and Galal Bakr Anis
Index 391
