This book offers a state-of-the-art, evidence-based reference to all aspects of veterinary cytology. Truly multidisciplinary in its approach, chapters are written by experts in fields ranging from clinical pathology to internal medicine, surgery, ophthalmology, and dermatology, drawing the various specialties together to create a comprehensive picture of cytology's role in diagnosis and treatment of animal disease. Firmly grounded in the primary literature, the book focuses on companion animals, with special chapters for species with fewer publications. Chapters are logically organized by body system, with additional chapters on tumors of particular import and diagnostic decision making.
The first two sections of Veterinary Cytology focus on cytology techniques, quality control, and special laboratory techniques. Subsequent sections are organ/tissue-based and reflect what is known about the canine, feline, and equine species. This is followed by chapters on non-traditional species, including exotic companion mammals, rabbits, cattle, camelids, non-human primates, reptiles and birds, amphibians, fish, invertebrates, and sheep and goats. The last section highlights some unique features of the applications of cytology in industry settings.
- Provides a gold-standard reference to data-driven information about cytologic analysis in companion animal species
- Brings together authors from a wide range of specialties to present a thorough survey of cytology's use in veterinary medicine
- Offers broader species coverage and greater depth than any cytology reference currently available
Veterinary Cytology is an essential resource for clinical and anatomic pathologists and any specialist in areas using cytology, including veterinary oncologists, criticalists, surgeons, ophthalmologists, dermatologists, and internists.
Table of Contents
Preface xi
Acknowledgments xiii
List of Contributors xv
List of Abbreviations xxiii
Part I Basic Cytology Techniques 1
1 Sample Collection 3
Kari L. Anderson, Angela Gwynn, Carrie A. Wood, and Leslie C. Sharkey
2 Routine Stains and Automated Stainers 12
Harold Tvedten
3 Microbiologic Review of Cytology Samples 18
Erin N. Burton, Sharon M. Dial, and Leslie C. Sharkey
4 Evidence-Based Cytology 30
Laura Adhikari
5 General Approach to Diagnostic Cytology 35
Jed Overmann
Part II Quality Control and Special Laboratory Techniques 41
6 Quality Assurance in Cytology 43
Liron Pantanowitz
7 Special Staining Techniques: Application and Quality Assurance 47
Kelly S. Santangelo, Deanna M.W. Schaefer, Sarah E. Leavell, and Heather L. Priest
8 Cell Block Preparation Techniques and Applications in Veterinary Medicine 73
Koranda A. Walsh and Reema T. Patel
9 Molecular Clonality Testing 79
Davis Seelig and Anne C. Avery
10 Cytogenetics 85
Matthew Breen
Part III Skin and Subcutis 95
11 Inflammatory Diseases of the Skin 97
Sandra Diaz and M. Judith Radin
12 Dermal and Subcutaneous Masses 115
Jennifer L. Brazzell, Daniel Heinrich, and Jillian Zientek Walz
13 Cutaneous Mast Cell Tumors 138
Maxey L. Wellman and Cheryl London
14 Plasma Cell Tumors 151
Maxey L. Wellman and William C. Kisseberth
15 Melanoma 158
Helen Michael
16 Soft Tissue Sarcomas 166
Jennifer D. Steinberg and John Keating
Part IV Ear and Eye 177
17 Ear Cytology 179
Susan Lowum, Sandra Nogueira Koch, and Leslie C. Sharkey
18 Collection of Ophthalmic Cytology Specimens 184
Michala de Linde Henriksen and Christine C. Lim
19 Ocular Cytology of the Dog 188
Cathy Trumel, Jean-Yves Douet, and Fanny Granat
20 Ocular Cytology of the Cat 205
Christine C. Lim and Jennifer L. Brazzell
21 Ocular Cytology of the Horse 222
Michala de Linde Henriksen and Leslie C. Sharkey
Part V Musculoskeletal System 241
22 Muscle 243
Catherine J. Benson
23 Bone and Periarticular Structures 249
Jessica Lawrence and Elspeth Milne
Part VI Respiratory System 259
24 Upper Respiratory Tract of the Dog and Cat 261
Janet Beeler-Marfisi, Alice Defarges Bichot, and Dorothee Bienzle
25 Lower Respiratory Tract of the Dog and Cat 281
Alice Defarges Bichot and Dorothee Bienzle
26 Respiratory Cytology of the Horse 302
Melissa Dawn Meachem and Julia Bettina Montgomery
Part VII Hemolymphatic System 317
27 Lymph Nodes 319
Stefano Comazzi, Luca Aresu, Jenna H. Burton, and Paul R. Avery
28 Spleen 342
Davis Seelig
29 Thymus 352
Cleverson D. Souza and Meredeth Crandall McEntire
Part VIII Gastrointestinal Tract 359
30 Oral Cavity 361
Jill Schappa Faustich, Kevin S. Stepaniuk, Nicholas A. Robinson, and Cesar Piedra-Mora
31 Esophagus and Stomach 380
Marian Taulescu, Irina Amorim, and Robert Washabau
32 Intestines and Rectum 394
Ugo Bonfanti
33 Fecal Cytology 407
Cathy Trumel and Olivier Dossin
Part IX Liver and Pancreas 411
34 Nonneoplastic Disorders of the Liver 413
Carlo Masserdotti
35 Hepatobiliary Neoplasia and Cancer Staging 432
Charles E. Wiedmeyer and Jeff Bryan
36 Pancreas 445
Leslie C. Sharkey and Sarah Crain
Part X Urinary Tract 455
37 Kidney 457
Camille A. McAloney and Leslie C. Sharkey
38 Urinary Bladder 466
Joyce S. Knoll and Mary Anna Labato
39 Urine Cytology 480
Nicole M. Weinstein
Part XI Reproductive Tract 499
40 Testes, Ovaries, and Prostate 501
Francisco de Oliveira Conrado and Sally E. Henderson
41 Evaluation of Semen 531
Scott Madill and Margaret V. Root Kustritz
42 Vaginal Cytology in the Bitch and Queen 552
Margaret V. Root Kustritz
43 Uterine Cytology 559
Paula M. Krimer and Doris M. Miller
44 Mammary Gland 582
Natalie Hoepp
Part XII Endocrine 595
45 Thyroid and Parathyroid Glands 597
M. Judith Radin and Maxey L. Wellman
46 Adrenal Gland 608
Walter Bertazzolo
Part XIII Central Nervous System 617
47 Central Nervous System Neoplasia in the Dog and Cat 619
Diana Schwartz, William Vernau, and Karen M. Vernau
48 Cerebrospinal Fluid Analysis in the Dog and Cat 638
Marlyn S. Whitney and Joan Ripley Coates
49 Cerebrospinal Fluid Analysis in Horses and Large Animals 655
Eric J. Fish and Joseph J. Bertone
Part XIV Fluid Analysis 665
50 Laboratory Techniques for Fluid Analysis 667
Linda M. Vap and Wendy S. Sprague
51 Pericardial Fluid 687
Shelley Burton and Etienne Côté
52 Abdominal and Thoracic Fluid Analysis in Dogs and Cats 695
Mary Anna Thrall
53 Abdominal and Thoracic Fluid Analysis in Horses 713
Emma Hooijberg and Catriona Lyle
54 Synovial Fluid Analysis of the Dog and Cat 727
R. Darren Wood and Thomas Gibson
55 Synovial Fluid Analysis of the Horse 736
R. Darren Wood and Judith Koenig
Part XV Species Specific Cytology 745
56 Exotic Companion Mammals 747
Courtney Johnson, Kyle Lauren Webb, Jennifer Graham, and Leslie C. Sharkey
57 Rabbit 766
Laurie Millward
58 Cattle 782
Amy L. Weeden, K. Lisa Hulme-Moir, and Julie Tomlinson
59 Camelids 800
Susan J. Tornquist, Christopher K. Cebra, and Michala de Linde Henriksen
60 Nonhuman Primates 809
Mark A. Suckow, Jodi A. Scholz, and Kirstin F. Barnhart
61 Reptiles and Birds 828
Nicole I. Stacy, Helene Pendl, and Peter M. Wencel
62 Amphibians 869
Allan P. Pessier
63 Fish 876
Charlotte Hollinger and Alisa L. Newton
64 Invertebrates 921
Charlotte Hollinger and Nicole I. Stacy
65 Sheep and Goats 929
Emma Hooijberg, Thomas Jenei, and Leslie C. Sharkey
Part XVI Applications of Cytology in Industry 937
66 Cytological Evaluation in Biomedical Research and Toxicity Studies 939
Florence Poitout-Belissent, Michelle Cora, and Angela Wilcox
Index 964
