Natural products have dominated our lives since ancient times. Today, they are an inexhaustible source of new medications for disease treatment. The practice of evaluating bioactive compounds extracted from natural sources has also advanced significantly, prompting a need to understand current methods to identify and evaluate them.
This book covers basic scientific aspects of preclinical research on natural products for specific conditions and diseases. These include aging, gynecological disorders, inflammatory disorders, renal disorders and cardiovascular disorders. Each of the 10 book chapters give a structured overview on preclinical methods on the etiology of diseases, natural products as the materials for the bioassays, extract types, concentration of the extracts/compounds for in vitro and in vivo assays, preparation of the test materials, application of the test materials, step-by-step methods and related calculations.
The book is intended as a quick reference for natural product researchers, pharmacists and postgraduate students in pharmacognosy. Medical doctors working in preclinical research on natural products will also benefit from the information provided.
Table of Contents
Chapter 1 Preclinical Activity Assessment of Natural Products on Related Activity
- In Vitro Assay Procedures
- Cuprac Assay
- Aim
- Principle
- Materials and Equipments Required
- Reagents Required
- Protocol
- Calculations
- Dpph Scavenging Activity
- Aim
- Principle
- Materials and Equipments Required
- Reagents Required
- Protocol
- Calculations
- Frap Assay
- Aim
- Principle
- Materials and Equipments Required
- Reagents Required
- Protocol
- Calculations
- Tpc Assay
- Aim
- Principle
- Materials and Equipments Required
- Reagents Required
- Protocol
- Calculations
- Abts Scavenging Activity
- Aim
- Principle
- Materials and Equipments Required
- Reagents Required
- Protocol
- Calculations
- No Scavenging Activity
- Aim
- Principle
- Material and Equipments Required
- Reagents Required
- Protocol
- Calculations
- Orac Assay
- Aim
- Principle
- Materials and Equipments Required
- Reagents Required
- Protocol
- Calculations
- So Scavenging Activity
- Aim
- Principle
- Materials and Equipments Required
- Reagents Required
- Protocol
- Calculations
- Bcb Assay
- Aim
- Principle
- Materials and Equipments Required
- Reagents Required
- Protocol
- Calculations
- Lipid Peroxidation Assay in Cells
- Aim
- Principle
- Materials and Equipments Required
- Reagents Required
- Protocol
- Calculations
- Antihaemolytic Activity
- Aim
- Principle
- Materials and Equipments Required
- Reagents Required
- Protocol
- Calculations
- Dna Nicking Assay
- Aim
- Principle
- Materials and Equipments Required
- Reagents Required
- Protocol
- Intracellular Ros Measurement by 2',7' Dichlorodihydrofluorescein Diacetate (Dcfh2- Da)
- Aim
- Principle
- Materials and Equipments Required
- Reagents Required
- Protocol
- Calculations
- In Vivo Assay Procedures
- Sod Activity
- Aim
- Principle
- Materials and Equipments Required
- Reagents Required
- Protocol
- Calculations
- Alternative Methods
- Cat Activity
- Aim
- Cat Activity in Biological Tissues
- Principle
- Materials and Equipments Required
- Reagents Required
- Protocol
- Calculations
- Cat Activity in Serum
- Principle
- Materials and Equipments Required
- Reagents Required
- Protocol
- Calculations
- Gpx Activity
- Aim
- Principle
- Materials and Equipments Required
- Reagents Required
- Protocol
- Calculations
- Alternative Methods
- Gr Activity
- Aim
- Principle
- Materials and Equipments Required
- Reagents Required
- Protocol
- Calculations
- Alternative Methods
- Reduced Gsh and Gssg Levels
- Aim
- Principle
- Materials and Equipments Required
- Reagents Required
- Protocol
- Calculations
- Alternative Methods
- Glutathione S-Transferase (Gsts) Activity
- Aim
- Principle
- Materials and Equipments Required
- Reagents Required
- Protocol
- Calculations
- Alternative Methods
- Γ-Glutamyl Transpeptidase (Ggt) Activity
- Aim
- Principle
- Materials and Equipments Required
- Reagents Required
- Protocol
- Calculations
- Alternative Methods
- Estimation of Lipid Peroxidation by Measuring Serum Mda Levels
- Aim
- Principle
- Materials and Equipments Required
- Reagents Required
- Protocol
- Calculations
- Alternative Methods
- Lactate Dehydrogenase (Ldh) Activity in Serum
- Aim
- Principle
- Materials and Equipments Required
- Reagents Required
- Protocol
- Calculations
- Trx and Thioredoxin Reductase (Trxr) Activity
- Aim
- Principle
- Materials and Equipments Required
- Reagents Required
- Protocol
- Calculations
- Peroxiredoxin Enzyme (Prx) Activity
- Aim
- Principle
- Materials and Equipments Required
- Reagents Required
- Protocol
- Protocol
- Protocol
- Calculations
- Nqo-1 Activity
- Aim
- Principle
- Materials and Equipments Required
- Reagents Required
- Protocol
- Calculations
Author
- Ipek Suntar
- Luigi Mella
- Davide Barreca
