Modifications and targeting of protein termini Part B. Methods in Enzymology Volume 686

Table of Contents

1. In vitro production of N-degron fused proteins and its applicationHyun Kyu Song, Bong Heon Kim, Leehyeon Kim, Do Hoon Kwon and Woo Seok Yang2. Identification of N-degrons and N-recognins using peptide pull-downs combined with quantitative mass spectrometry-based proteomicsFranziska M?ller and Tanja Bange3. Monitoring ADO-dependent proteolysis in cells using fluorescent reporter proteinsThomas P. Keeley4. Monitoring the interactions between N-degrons and N-recognins of the Arg/N-degron pathwayYong Tae Kwon, Ah Jung Heo, Soon Chul Kwon and Jihoon Lee5. Characterization and chemical modulation of p62/SQSTM1/Sequestosome-1 as an autophagic N-recognin of the Arg/N-degron pathwaySu Jin Lee, Hye Yeon Kim, Min Ju Lee, Su Bin Kim, Yong Tae Kwon and Chang Hoon Ji6. Characterization of degradation signals at protein C-terminiHsueh-Chi S. Yen, Chi-Wei Yeh, Wei-Chieh Huang, Shu-Yu Hsu, Ching-Yu Chu and Lo-Tung Lee7. Analysis of higher plant N-degron pathway components and substrates via expression in S. cerevisiaeJessica B?hm, Nikola Winter, Aida Kozlic, Theresia Telser, Lilian Nehlin and Andreas Bachmair8. Building libraries to dissect terminal degrons with fluorescent timersAnton Khmelinskii, Ka-Yiu Edwin Kong, Christian Reinbold and Michael Knop9. Affinity isolation and biochemical characterisation of N-degron ligands using the N-recognin, ClpSKaye N. Truscott and David A. Dougan10. Probing the effects of N-terminal acetylation on a-synuclein structure, aggregation and toxicityJanet R. Kumita, Rosie Bell and Michele Vendruscolo11. Increasing the coverage of the N-terminome with Lys-N Amino Terminal enrichment (LATE)Oded Kleifeld, Rawad Hanna, Andrey Rozenberg and Tali Lavy12. In vitro autoubiquitination activity of E3 ubiquitin ligases of the N-degron pathwayNico Dissmeyer13. Antibodies specific for N-terminal arginylationNico Dissmeyer14. TEV protease cleavage in generation of artificial substrate proteins bearing neo-N-terminiNico Dissmeyer and Lioba Inken Winckler15. Multiplexed protein stability (MPS) profiling of terminal degrons using fluorescent timer libraries in Saccharomyces cerevisiaeMichael Knop, Christian Reinbold, Ka-Yiu Edwin Kong, Ilia Kats and Anton Khmelinskii16. Using cell lysates to assess N-terminal acetyltransferase activity and impairmentThomas Arnesen, Nina McTiernan and Malin Lundekvam