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DNA Nanotechnology for Cell Research. From Bioanalysis to Biomedicine. Edition No. 1

  • Book

  • 544 Pages
  • March 2024
  • John Wiley and Sons Ltd
  • ID: 5879330
DNA Nanotechnology for Cell Research

Comprehensive coverage of DNA nanotechnology with a focus on its biomedical applications in disease diagnosis, gene therapy, and drug delivery

Bringing together multidisciplinary aspects of chemical, material, and biological engineering, DNA Nanotechnology for Cell Research: From Bioanalysis to Biomedicine presents an overview of DNA nanotechnology with emphasis on a variety of different applications in cell research and engineering, covering a unique collection of DNA nanotechnology for fundamental research and engineering of living cells, mostly in cellulo and in vivo, for the first time. Broad coverage of this book ranges from pioneering concepts of DNA nanotechnology to cutting-edge reports regarding the use of DNA nanotechnology for fundamental cell science and related biomedical engineering applications in sensing, bioimaging, cell manipulation, gene therapy, and drug delivery.

The text is divided into four parts. Part I surveys the progress of functional DNA nanotechnology tools for cellular recognition. Part II illustrates the use of DNA-based biochemical sensors to monitor and image intracellular molecules and processes. Part III examines the use of DNA to regulate biological functions of individual cells. Part IV elucidates the use of DNA nanotechnology for cell-targeted medical applications.

Sample topics covered in DNA Nanotechnology for Cell Research include: - Selections and applications of functional nucleic acid toolkits, including DNA/RNA aptamers, DNAzymes, and riboswitches, for cellular recognition, metabolite detection, and liquid biopsy. - Developing intelligent DNA nanodevices implemented in living cells for amplified cell imaging, smart intracellular sensing, and in cellulo programmable biocomputing. - Harnessing dynamic DNA nanotechnology for non-genetic cell membrane engineering, receptor signaling reprogramming, and cellular behavior regulation. - Construction of biocompatible nucleic acid nanostructures as precisely controlled vehicles for drug delivery, immunotherapy, and tissue engineering.

Providing an up-to-date tutorial style overview along with a highly valuable in-depth perspective, DNA Nanotechnology for Cell Research is an essential resource for the entire DNA-based nanotechnology community, including analytical chemists, biochemists, materials scientists, and bioengineers.

Table of Contents

Preface xv

Part I DNA Nanotechnology for Cellular Recognition (Cell SELEX, Cell Surface Engineering) 1

1 Developing DNA Aptamer Toolbox for Cell Research 3
Liang Yue, Shan Wang, and Weihong Tan

1.1 Cells and Their Complexity 3

1.2 Features and Advantages of DNA Aptamers 4

1.3 On-demand Synthesis and Screening of DNA Aptamers 5

1.4 Toward a Toolbox of DNA Aptamers for Cellular Applications 9

1.4.1 Chemical Modifications via Solid-Supported Synthesis Strategy 10

1.4.2 Chemical Modifications Through Covalent Conjugation 13

1.4.3 Self-assembly Systems Based on Chemically Modified DNA Aptamers 15

1.4.4 DNA Aptamers Engineered with Nanotechnology 19

1.5 Summary and Outlook 21

Acknowledgments 22

References 22

2 Bacterial Detection with Functional Nucleic Acids: Escherichia coli as a Case Study 31
Yash Patel and Yingfu li

2.1 General Introduction to Bacteria 31

2.2 E. coli 32

2.3 Conventional Methods for General E. coli Detection 33

2.3.1 Sorbitol MacConkey Agar 34

2.3.2 Pcr 34

2.4 Biosensors for E. coli Detection 35

2.4.1 Protein Biosensors for E. coli Detection 35

2.4.2 Functional Nucleic Acid-Based Sensors for E. coli Detection 36

2.5 Conclusion 41

References 42

3 From Ligand-Binding Aptamers to Molecular Switches 47
Sanshu Li, Xiaojun Zhang, Tao Luo, Xuejiao Liu, Tingting Zhai, and Hongzhou Gu

3.1 Aptamers Can Be Generated by SELEX 47

3.2 Various Subtypes of SELEX Have Been Invented 48

3.3 Riboswitches Are Natural RNA Aptamers Carrying Expression Platforms 49

3.4 Riboswitches Use Various Mechanisms to Regulate Gene Expression 52

3.5 Riboswitches Are Potential Drug Targets 55

3.6 Fusing Aptamer with Expression Platform to Construct Artificial RNA Switches 55

3.7 Conclusions 57

Acknowledgments 57

References 57

4 DNA Nanotechnology-Based Microfluidics for Liquid Biopsy 63
Qi Niu, Shanqing Huang, Chaoyong Yang, and Lingling Wu

4.1 Introduction 63

4.2 DNA Nanotechnology-Based Microfluidics for Isolation of Circulating Targets 65

4.2.1 Aptamer-Modified Micro/Nano-Substrate Microfluidic Chip 65

4.2.2 DNA Framework-Supported Affinity Substrate Microfluidic Chip 73

4.3 DNA Nanotechnology-Based Microfluidics for Release and Detection of Circulating Targets 77

4.3.1 Efficient Release 77

4.3.2 Analysis and Destruction of CTCs 81

4.3.3 Sensitive Detection of EVs 85

4.4 DNA-Assisted Microfluidics for Single-Cell/Vesicle Analysis 88

4.4.1 Single-Cell Analysis by Sequencing 89

4.4.2 Single-Cell Analysis by Spectroscopy 93

4.4.3 Single-Vesicle Analysis 95

4.5 Summary and Outlook 97

References 98

5 Spatiotemporal-Controlled Cell Membrane Engineering Using DNA Nanotechnology 105
Wenxue Xie, Cong Ren, Minjie Lin, and Hang Xing

5.1 Background 105

5.2 DNA Modifications on the External Cell Membrane Surface 107

5.2.1 Strategies to Incorporate DNA onto External Cell Membrane Surface 107

5.2.2 Applications of External Membrane-modified DNA 114

5.3 DNA Modifications on the Internal Cell Membrane Surface 120

5.3.1 Transmembrane Modification of DNA 121

5.3.2 Inner Leaflet Modification Approaches 124

5.3.3 Liposome Fusion-Based Transport (LiFT) Approach 129

5.4 Perspectives 130

Acknowledgments 132

References 132

Part II Dna Nanotechnology for Cell Imaging and Intracellular Sensing 141

6 Metal-Dependent DNAzymes for Cell Surface Engineering and Intracellular Bioimaging 143
Ruo-Can Qian, Yuting Wu, Zhenglin Yang, Weijie Guo, Ze-Rui Zhou, andYiLu

6.1 Cellular Surface Engineering and Intracellular Bioimaging Show Great Potential in Biological and Medical Research 143

6.2 Metal-Specific DNAzymes: A Suitable Choice for Artificial Manipulation of Living Cells 144

6.3 Cell Surface Engineering by Programmable DNAzymes 145

6.3.1 Cell Surface Engineering Using DNAzyme-Based Control Switches 145

6.3.2 Dynamic Inter- and Intra-Cellular Regulation Using Engineered DNAzyme Molecular Machines 147

6.3.3 Cell Surface Imaging of Extracellular Signaling Molecule Using DNAzyme Sensors 149

6.4 Intracellular Imaging of Metal Ions with DNAzyme-Based Biosensors 150

6.4.1 DNAzyme-Based Catalytic Beacon for Intracellular Imaging 150

6.4.2 Caged DNAzymes for Temporally Controlled Imaging 154

6.4.3 DNAzyme-Based Sensing with Signal Amplification 159

6.4.4 Genetically Encoded Sensors for Metal Sensing in Living Cells 159

6.5 Conclusion 161

Acknowledgments 161

References 161

7 DNA Nanomotors for Bioimaging in Living Cells 169
Hanyong Peng, Aijiao Yuan, Hang Xiao, Zi Ye, Lejun Liao, Shulin Zhao, and X. Chris Le

References 184

8 Illuminating RNA in Live Cells with Inorganic Nanoparticles-Based DNA Sensor Technology 189
Fangzhi Yu, Xiangfei li, Xiulin Yi, and Lele li

8.1 RNA Detection and Imaging 189

8.2 RNA Imaging Based on Direct Hybridization 190

8.3 RNA Imaging Based on Strand Displacement Reactions 192

8.4 Signal-amplified RNA Imaging 194

8.4.1 HCR-Based DNA Nanosensors for Amplified RNA Imaging 194

8.4.2 CHA-Based DNA Nanosensors for Amplified RNA Imaging 196

8.4.3 Amplified RNA Imaging Based on DNA Nanomachines 198

8.5 Spatiotemporally Controlled RNA Imaging in Live Cells 203

8.6 Conclusion 206

Acknowledgment 207

References 207

9 Building DNA Computing System for Smart Biosensing and Clinical Diagnosis 211
Jiao Yang and Da Han

9.1 DNA Computing 211

9.1.1 DNA Logic Gates Based on Functional DNA Motifs 212

9.1.2 DNA Logic Gates Based on DNA Cascading Reactions 215

9.2 DNA-Based Computing Devices for Biosensing 217

9.2.1 In Vitro Biosensing 217

9.2.2 Cellular Biosensing 220

9.3 DNA Computing for Clinical Diagnosis 223

9.4 Conclusion 226

References 226

10 Intelligent Sense-on-Demand DNA Circuits for Amplified Bioimaging in Living Cells 233
Yuqiu He, Zeyue Wang, Yuqian Jiang, and Fuan Wang

10.1 DNA Circuit: The Promising Technique for Bioimaging 233

10.2 Nonenzymatic DNA Circuits 234

10.2.1 Hybridization Chain Reaction (HCR) 234

10.2.2 Catalytic Hairpin Assembly (CHA) 235

10.2.3 Entropy-Driven DNA Catalytic Reaction (EDR) 236

10.2.4 DNAzyme-Powered Catalytic Reaction (DZR) 236

10.3 Intelligent Integrated DNA Circuits for Amplified Bioimaging 237

10.3.1 Hybridization-Dependent Cascade DNA Circuits 237

10.3.2 DNAzyme-Assisted Tandem DNA Circuits 239

10.3.3 Autocatalysis-Driven Feedback DNA Circuits 241

10.4 Stimuli-Responsive DNA Circuits for Reliable Bioimaging 243

10.4.1 Photo-Responsive DNA Circuits for Amplified Bioimaging 243

10.4.2 Enzyme-Activated DNA Circuits for Amplified Bioimaging 245

10.4.3 RNA-Stimulated DNA Circuits for Amplified Bioimaging 246

10.4.4 Other Strategies for Regulating DNA Circuits 249

10.5 Conclusion and Perspectives 251

Acknowledgments 252

References 252

11 DNA Nanoscaffolds for Biomacromolecules Organization and Bioimaging Applications 259
Yuanfang Chen, Jiayi Li, and Yuhe R. Yang

11.1 Introduction 259

11.2 Assembly of DNA-Scaffolded Biomacromolecules 259

11.3 Application of DNA Nanoscaffold for Regulation of Enzyme Cascade Reaction 261

11.3.1 Distance Control of Enzyme Cascade 261

11.3.2 Enzyme Compartmentalization 263

11.3.3 Directed Substrate Channeling with Swinging Arms 264

11.3.4 Scaffolded Enzyme Cascade in Living Cells 265

11.4 DNA Nanostructures Empowered Bioimaging Technologies 267

11.4.1 DNA Nanostructures Scaffolded Fluorophore Expansion 267

11.4.2 DNA-PAINT-Based Super-Resolution Fluorescence Imaging 269

11.4.3 DNA Nanostructures-Assisted Cryogenic Electron Microscopy Characterization 269

11.5 Summary and Outlook 270

References 271

Part III Dna Nanotechnology for Regulation of Cellular Functions 279

12 Adopting Nucleic Acid Nanotechnology for Genetic Regulation In Vivo 281
Friedrich C. Simmel

12.1 Introduction 281

12.2 Toehold-Mediated Strand Displacement: Switching Nucleic Acids with Nucleic Acids 282

12.3 Toehold Riboregulators and Related Systems 283

12.3.1 Riboswitches 283

12.3.2 Translational Switching with Toehold Switches 284

12.3.3 Toehold Switching in Eukaryotes 285

12.3.4 Transcriptional Switching 286

12.3.5 Applications as Sensors 286

12.3.6 Applications in Biocomputing 287

12.4 Applying Nucleic Acid Nanotechnology to CRISPR and RNA Interference 288

12.4.1 CRISPR Techniques 288

12.4.2 Switchable Guide RNAs 289

12.4.3 Implementing More Complex Programs in Mammalian Cells 291

12.4.4 Combining CRISPR with Origami 292

12.4.5 MicroRNAs and RNA Interference 292

12.5 Delivery of Nucleic Acid Devices, In Vivo Production, and Challenges for In Vivo Operation 293

12.5.1 Delivery 293

12.5.2 In Vivo Production 293

12.5.3 Challenges 294

12.6 Conclusion and Outlook 294

Acknowledgments 295

References 295

13 Cell Membrane Functionalization via Nucleic Acid Tools for Visualization and Regulation of Cellular Receptors 303
Shan Chen, Jingying Li, and Huanghao Yang

13.1 Nucleic Acid-Based Functionalization Strategies: From Receptor Information to DNA Probes 303

13.2 Uncovering Molecular Information of Cellular Receptors 307

13.3 Governing Cellular Receptors-Mediated Signal Transduction 313

13.4 Conclusion 318

Acknowledgments 318

References 318

14 Harnessing DNA Nanotechnology for Nongenetic Manipulation and Functionalization of Cell Surface Receptor 325
Hexin Nan, Hong-Hui Wang, and Zhou Nie

14.1 Introduction 325

14.2 Principle of DNA-enabled Molecular Engineering for Receptor Regulation 329

14.2.1 Recognition Module for Receptor Manipulation 330

14.2.2 Spatial Scaffold Module for Receptor Organization 330

14.2.3 Dynamic Assembly Module for Kinetic Control of Receptor 331

14.3 DNA Nanodevices for Programming Receptor Function 332

14.3.1 Bivalent Aptamer Mimicking Natural Ligand to Induce Receptor Dimerization 332

14.3.2 DNA Nanodevices to Customize Receptor Responsiveness 333

14.3.3 Light-Responsive DNA Nanodevices for Spatiotemporal Receptor Regulation 335

14.3.4 DNA Nanodevices for Visualization of Receptor Activation 337

14.4 Elaborate and Intelligent DNA Nanodevices Reprogramming Receptor Function 339

14.4.1 Mechanical Control Over Receptor-Mediated Cellular Behavior 339

14.4.2 Precise Cell Targeting for Selective Receptor Modulation 341

14.4.3 Spatial Organization of Nanoscale Receptor Distribution 344

14.5 Conclusions and Perspectives 347

Acknowledgments 348

References 348

15 DNA-Based Cell Surface Engineering for Programming Multiple Cell-Cell Interactions 355
Mingshu Xiao, Yueyang Sun, Li Li, and Hao Pei

15.1 DNA Nanotechnology: The Tool of Choice for Programming Cell-Cell Interactions 355

15.2 Modifying Cell Surface with DNA 356

15.3 Programming Cell-Cell Interactions by DNA Nanotechnology 359

15.3.1 Ligand-Receptor Binding-Based Cell-Cell Interactions 359

15.3.2 DNA Hybridization-Based Cell-Cell Interactions 362

15.3.3 DNA Circuit-Regulated Cell-Cell Interactions 364

15.4 Conclusion 366

Acknowledgments 367

References 367

16 Designer DNA Nanostructures and Their Cellular Uptake Behaviors 375
Jing Ye, Donglei Yang, Chenzhi Shi, Fei Zhou, and Pengfei Wang

16.1 Introduction 375

16.2 DNA Nanotechnology 376

16.2.1 The Beginning of DNA Nanotechnology 376

16.2.2 DNA Origami 377

16.2.3 Single-Stranded DNA Tiles 378

16.2.4 Dynamic DNA Structures 379

16.3 Pathways of Cell Endocytosis 381

16.3.1 Clathrin-Mediated Endocytosis 381

16.3.2 Clathrin-Independent Endocytosis 382

16.3.3 Phagocytosis 384

16.3.4 Macropinocytosis 384

16.3.5 Caveolin-Mediated Endocytosis 385

16.4 Analysis of DNA Nanostructures’ Cellular Uptake Behaviors 386

16.4.1 Effect of Size and Shape on Cellular Uptake 386

16.4.2 Effect of Surface Modifications on Cellular Uptake 389

16.4.3 Effect of Other Aspects on Cellular Uptake 392

References 395

Part IV Dna Nanotechnology for Cell-targeted Medical Applications 401

17 Toward Production of Nucleic Acid Nanostructures in Life Cells and Their Biomedical Applications 403
Mengxi Zheng, Victoria E. Paluzzi, Cuizheng Zhang, and Chengde Mao

17.1 DNA Nanostructures 403

17.1.1 Strategies of DNA Nanostructures Construction 403

17.1.2 Production of ssDNA Nanostructures in Living Cells 404

17.2 RNA Nanostructures 406

17.2.1 Strategies of RNA Nanostructures Construction 406

17.2.2 Production of ssRNA Nanostructures in Living Cells 407

17.3 Applications 409

17.4 Conclusion 412

References 412

18 Engineering Nucleic Acid Structures for Programmable Intracellular Biocomputation 415
Na Wu, Pengyan Hao, Chunhai Fan, and Yongxi Zhao

References 432

19 DNA Supramolecular Hydrogels for Biomedical Applications 437
Ziwei Shi, Yuanchen Dong, and Dongsheng Liu

19.1 Introduction 437

19.2 Classification and Preparation of DNA Supramolecular Hydrogels 438

19.2.1 Pure DNA Supramolecular Hydrogels 438

19.2.2 Hybrid Supramolecular DNA Hydrogels 440

19.3 Biomedical Application of DNA Supramolecular Hydrogels 443

19.3.1 DNA Supramolecular Hydrogels for Bio-sensing 443

19.3.2 DNA Supramolecular Hydrogels for Drug Delivery 446

19.3.3 DNA Supramolecular Hydrogels for Immunotherapy 449

19.3.4 DNA Supramolecular Hydrogels for 3D Cell Culture 451

19.3.5 DNA Supramolecular Hydrogels for Tissue Engineering 454

19.4 Conclusions and Perspectives 458

References 459

20 Rolling Circle Amplification-Based DNA Nanotechnology for Cell Research 467
Nachuan Song, Yiwen Chu, Xun You, and Dayong Yang

20.1 Introduction 467

20.2 Principle and Synthetic Methods of RCA 468

20.2.1 Principle 468

20.2.2 DNA Hydrogel 469

20.2.3 DNA Nanoparticles 469

20.3 RCA-Based DNA Nanotechnology for Cell Separation 469

20.4 RCA-Based DNA Nanotechnology for Nucleic Acid Drug Delivery 475

20.5 Conclusion 484

Acknowledgment 485

References 485

21 Precise Integration of Therapeutics in DNA-Based Nanomaterials for Cancer Treatments 489
Yimeng Li, Lijuan Zhu, and Chuan Zhang

21.1 DNA-Based Nanomaterials in Biomedicine 490

21.1.1 Properties of DNA-Based Nanomaterials 491

21.1.2 Architectures of DNA-Based Nanomaterials 492

21.1.3 Interactions Between DNA-Based Drug Delivery Systems (DDSs) and Cells 495

21.2 Strategies on Constructing DNA-Based DDSs 498

21.2.1 DNA-Based DDSs Engineered Through Non-covalent Interactions 499

21.2.2 DNA-Based DDSs Engineered Through Covalent Interactions 502

21.3 Precise Integration of Therapeutics into DNA-Based DDSs to Achieve Synergistic Cancer Treatment 507

21.3.1 Chemogenes 507

21.3.2 Chemogene-Based DNA Nanomaterials 508

References 511

Index 515

Authors

Zhou Nie Hunan University, China.