Enzymes in RNA Science and Biotechnology, Part B, Volume 692 in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on topics such as Quantitative base-resolution sequencing technology for mapping pseudouridines in mammalian mRNA, Quantitative base-resolution DAMM-seq for mapping RNA methylations in tRNA and mitochondrial dsRNA, Discovering RNA modification enzymes using a comparative genomics approach, Functional analysis of tRNA modification enzymes using mutational profiling, and Fluorescent labeling of tRNA for rapid kinetic interaction studies with tRNA-binding proteins.
Other chapters cover Enzymatic Synthesis of RNA Standards for Mapping and Quantifying RNA Modifications in Sequencing Analysis, Characterizing RNase L-mediated mRNA decay in single cells, Characterization of RNase J, Pri-miRNA cleavage assays for the Microprocessor complex, The pre-miRNA cleavage assays for DICE, Methods for study of ribonuclease targeting chimeras, and Profiling the in vivo RNA interactome associated with the endoribonuclease RNase III in Staphylococcus aureus.
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Table of Contents
Post-transcriptional modification enzymes1. Quantitative base-resolution sequencing technology for mapping pseudouridines in mammalian mRNA
Li-Sheng Zhang
2. Quantitative base-resolution DAMM-seq for mapping RNA methylations in tRNA and mitochondrial dsRNA
Li-Sheng Zhang
3. Discovering RNA modification enzymes using a comparative genomics approach
Satoshi Kimura
4. Functional analysis of tRNA modification enzymes using mutational profiling
Ryota Yamagami and Hiroyuki Hori
5. Fluorescent labeling of tRNA for rapid kinetic interaction studies with tRNA-binding proteins
Ute Kothe and Sarah Schultz
6. Enzymatic Synthesis of RNA Standards for Mapping and Quantifying RNA Modifications in Sequencing Analysis
Howard Gamper, Caroline McCormick, Amr Makhamreh, Meni Wanunu, Sara Rouhanifard and Ya-Ming Hou
Nucleases
7. Characterizing RNase L-mediated mRNA decay in single cells
James Burke, Renee Cusic and Monty Watkins
8. Characterization of RNase J
Balasubramanian Gopal, Muralidharan Vandanashree and Ankur Singh
9. Pri-miRNA cleavage assays for the Microprocessor complex
Tuan Anh Nguyen, Cong Truc Le and Thi Nhu Y. Le
10. The pre-miRNA cleavage assays for DICER
Tuan Anh Nguyen, Cong Truc Le and Thi Nhu Y. Le
11. Methods for study of ribonuclease targeting chimeras
Matt Disney, Noah Springer, Samatha Meyer, Yuquan Tong, Jessica Childs-Disney and Raphael Isaac Benhamou
12. Profiling the in vivo RNA interactome associated with the endoribonuclease RNase III in Staphylococcus aureus
Winton Wu, Chi Nam Ignatius Pang, Jai Tree and Daniel Giuseppe Mediati
