Terpene Synthases, Volume 700 in the Methods in Enzymology series, continues the legacy of this highly respected laboratory standard with its first dedicated collection on this important family of enzymes. Terpene synthases are a diverse set of enzymes that use exquisite mechanisms to form complex (poly)cyclic hydrocarbon skeletons. Chapters in this new volume include Structural analysis by X-ray crystallography and cryo-EM, Understanding mechanisms using stable isotopes, substrate analogs, or computational tools, Engineering fusion enzymes, Ancestral terpene cyclases, as well as the Sequence, structure, and function of non-canonical terpene synthases.
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Table of Contents
1. Methods for the Expression, Purification, and Structural Analysis of a Bifunctional Class I Diterpene Synthase, Fusicoccadiene Synthase2. Methods for the Expression, Purification, and Structural Analysis of a Bifunctional Class II Diterpene Synthase, Copalyl Diphosphate Synthase
3. X-ray Crystallography of Terpene Synthases
4. Isotopic Labellings for Mechanistic Studies
5. Understanding Mechanisms of Terpene Synthases Using Substrate Analogs
6. Docking Carbocations into Terpene Synthase Active Sites using Chemically Meaningful Constraints The Terdockin Approach
7. A Practical Tutorial/Guide to EnzyDock
8. Bioinformatics of Terpene Synthases
9. Ancestral Terpene Cyclases: From Fundamental Science to Applications in Biosynthesis
10. Coral Terpene Synthases
11. Deciphering Magnesium Binding Site and Structure-Function Insights in Class II Sesquiterpene Cyclase
12. Functional Characterization of the UbiA-Family of Terpene Synthases
13. Functional Analysis of Transmembrane Terpene Cyclases Involved in Fungal Meroterpenoid Biosynthesis
14. Vanadium Haloperoxidases as Noncanonical Terpene Synthases
15. Structure, Function, and Engineering of Large Terpene Synthases
16. Coral Terpene Synthases (and perhaps some genomics and genome mining)
17. Engineering Terpene Synthases via Substrate Analogs
18. Engineering Terpene Synthase Fusions Enzymes
