Research Methodology and Project Management in Biotechnology is a vital resource addressing core concepts in the dynamic field of biotechnology. This comprehensive textbook focuses on research methodology, techniques, and project management, and provides essential knowledge for students and faculty in life sciences and allied disciplines.
Key features of the book include learning objectives, self-assessments and exercises, and a simple presentation (using bullet points, tables, and figures) designed to assist comprehension and retention of key information.
Key features of the book include learning objectives, self-assessments and exercises, and a simple presentation (using bullet points, tables, and figures) designed to assist comprehension and retention of key information.
The book is split into 5 units with 12 focused chapters:
Unit I: Molecular Biology Techniques
- Covers various techniques used in molecular biology, including nucleic acid isolation, DNA fragmentation, PCR, DNA sequencing, and more.
Unit II: Scientific Communication and Literature
- Introduces the process of research writing.
Unit III: Biotechnology Entrepreneurship and Marketing
- Covers the role of funding, intellectual property rights, and regulations.
Unit IV: Genomics, Proteomics, and Bioinformatics
- Explores DNA sequencing strategies, gene expression analysis, and the role of bioinformatics in drug discovery.
Unit V: Advanced Biotechnological Techniques
- Covers topics such as antisense technology, molecular cytogenetics, pharmacogenomics, next-generation DNA sequencing, and ethical considerations in science and technology.
Unit VI: Medical Biotechnology
- Covers disease detection and diagnosis, genetic diseases, personalized medicine, nanotechnology, gene therapy, regenerative medicine, and the Human Genome Project.
Readership
Students and faculty in life sciences and allied courses.Table of Contents
- Contents
- Preface
- Learning Objectives
- Isolation and Separation of Nucleic Acid
- Restriction Mapping of Dna Fragments
- Pcr
- Blotting Techniques
- Dna Sequencing
- Gene Probe
- Exercise
- Conclusion
- References
- Learning Objectives
- The Human Genomic Project
- Human Genome
- Hap Map Project
- Encode (Encyclopedia of Dna Elements)
- Human Epigenome Project
- Recombinant Dna Technology
- Definition
- Recombinant Dna Molecule
- Objective of Producing Recombinant Dna Molecule
- Dna Technology
- Basic Tools for Dna Cloning
- Restriction Enzymes
- Nomenclature
- Recognition
- Recognition Sequence
- Cleavage Patterns
- Recombination of Dna Fragments
- Dna Ligase
- Vectors
- Cloning Vector
- Expression Vector
- Gene Cloning
- Choice of Vector
- Transformation of Host Organism
- Recombination Vectors Screening
- Clone Selection
- Dna Libraries
- Genomic Library
- Cdna Library
- Genomic Library
- Cdna Library
- Colony Hybridization for Screening of Libraries
- Searching Specific Genes or Cdna Molecules in Libraries
- Applications of Dna Technology
- Foreign Genes: Expression
- Dna Transfer into Eukaryotic Cells and Mammalian Embryos
- Transduction
- Transfection
- Electroporation
- Lipofection
- Introduction of Foreign Genes into Cells
- Transgenic Animals Can Be Made by Injecting Dna Directly
- Into Embryos
- Transgenic Mice
- Transgene Expression Can Be Targeted to Specific Tissues
- Mice With Altered Genomes Can Be Created by Injecting Esc
- Into Embryos
- Homologous Recombinations to Knock Out Genes in Mice
- Gene Therapy May Permit Manipulation of Gene Expression
- In Humans
- Analysing Genes
- Terminology
- Aims of Genomics
- Gene Mapping
- Fish
- Genetic Map
- Types of Genetic Maps
- Linkage Map
- Cytogenetic Map
- Physical Map
- Identification of Genes in Cloned Dna
- Htf/Cpg Islands
- Zoo Blots
- Exon Trapping
- Expressed Sequence Tags (Ests)
- Analysis of Human Genome Database
- Positional Cloning
- Candidate Genes
- Analysis of G Ene Expression
- Gene Expression
- Terminology
- Analysis of Transcription
- Two Important Points Concerning Measurement of Mrna Are
- Analysis of Whole Genome
- Intergenic Dna
- Ssrs
- Uses of Strs
- Evolution of Strs
- Sequence Variation
- Techniques for Studying Analysis of Products of a Single Gene
- Northern Blot
- Advantage
- Disadvantage
- Rnase Protection Assay
- Advantage
- Disadvantage
- Reverse Transcription Pcr (Rt-Pcr)
- Real-Time Rt-Pcr
- In Situ Hybridization
- Uses:
- Primer Extension Assay
- Techniques for Study of Complex Samples (Comparing
- Transcriptomes)
- Differential Screening
- Subtractive Hybridization
- Use
- Disadvantage
- Differential Display
- Array-Based Method
- Whole Genome Arrays Available
- Est Arrays
- Pcr Based Arrays
- Translational Analysis
- Western Blot
- Immunochemistry and Immunohistochemistry
- 2-D-Electrophoresis
- Isoelectric Focussing
- Alternatives to 2 De
- Mass Spectrometry (Ms)
- Two Strategies to Annotate Proteins
- Three Components of Ms
- Protein Arrays and Protein Chips
- General Protein Array
- Molecular Imprints or Protein Chips
- Molecularly Imprinted Polymers (Mips)
- Broad-Capture Agent Biochips
- Solution Arrays
- Dna Finger Printing and Forensic Analysis
- Introduction
- Dna Fingerprint
- Dna Typing
- How is Dna Typing Performed?
- Preparing a Dna Fingerprint
- Specimen Collection
- Precautions at Crime Scene
- Enemies of Evidence
- Ideal Sample
- Sources of Contamination of Dna Evidence
- Agencies That Provide Accreditation to Forensic Labs
- Rflp Analysis
- Pcr and Dna Amplification
- Dot Blot Analysis
- Str (Short Tandem Repeats) Analysis
- Mass Fatality Identification System (M-Fi Sys)
- Dna Profiling and Familial Relationships
- Debate
- Mitochondrial Dna (Mt Dna) Analysis
- Y- Chromosome Analysis
- Other Advances in Dna Profiling
- Analysis of Non-Human Dna
- Setting Up a New Lab
- Introduction to Biotechnology Lab Notes and Labels
- Chemical Handling and Storage
- Maintenance of Equipment
- Preparation and Storage of Buffers, Reagents
- Culture: Care and Maintenance
- Culture Preservation
- Precautions- Reasons
- Nucleic Acid Storage
- Lab Safety
- Lab Safety Plans
- Rules of Lab
- Good Lab Practice (Glp)
- Test Facilities
- Study
- Test Guidelines
- Test System
- Raw Data
- Specimen
- Test Substance
- Reference Substance
- Sample
- Batch
- Vehicle
- Organization of Test Facilities
- Quality Assurance (Qa)
- Facilities
- Infrastructure
- Test Systems
- Standard Operating Procedures
- Components of Test System
- Quality Management in Lab
- Quality Management
- Quality Management System (Qms)
- Accreditation
- Accreditation Body
- Standards
- Assessors
- Laboratory
- International Standards
- Three Iso Standards
- Accreditation Bodies and Standards
- Unsatisfactory Aspect: Iso
- Actions in Quality Management
- Documentation
- Documentation
- Document Control
- Control of Records and Clinical Material
- Review and Improvement
- Internal Audit
- External Audit
- Cooperative Audit
- Inputs to a Management Review
- Safety in Biomedical Research Laboratories
- Internet Resources on Lab Safety
- Training in Safety
- Biological Safety
- Biohazards in Lab
- Chemical Safety
- Regulations Related to Chemical Safety
- Msds: Material Safety Data Sheet
- Chemical Hazards
- Explosive
- Accidental
- Electrical Safety
- Fire Safety
- Physical Safety
- Sources of Physical Injury in Lab
- Radiological Safety
- Biological Hazards of Dna Technology
- Ways to Minimize Biohazards Physical Containment Methods
- Biological Containment Methods
- Student’S Activity
- Exercise
- Conclusion
- Consent of Publication
- Conflict of Interest
- Acknowledgment
Author
- Simmi Kharb
- Akshat Singh
- Abhyuday Singh
- Deepa Kharb
